Details of the Target

General Information of Target

Target ID LDTP01030
Target Name Peroxisome biogenesis factor 10 (PEX10)
Gene Name PEX10
Gene ID 5192
Synonyms
RNF69; Peroxisome biogenesis factor 10; EC 2.3.2.27; Peroxin-10; Peroxisomal biogenesis factor 10; Peroxisome assembly protein 10; RING finger protein 69
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPAAASPPEVIRAAQKDEYYRGGLRSAAGGALHSLAGARKWLEWRKEVELLSDVAYFGL
TTLAGYQTLGEEYVSIIQVDPSRIHVPSSLRRGVLVTLHAVLPYLLDKALLPLEQELQAD
PDSGRPLQGSLGPGGRGCSGARRWMRHHTATLTEQQRRALLRAVFVLRQGLACLQRLHVA
WFYIHGVFYHLAKRLTGITYLRVRSLPGEDLRARVSYRLLGVISLLHLVLSMGLQLYGFR
QRQRARKEWRLHRGLSHRRASLEERAVSRNPLCTLCLEERRHPTATPCGHLFCWECITAW
CSSKAECPLCREKFPPQKLIYLRHYR
Target Bioclass
Enzyme
Family
Pex2/pex10/pex12 family
Subcellular location
Peroxisome membrane
Function
E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX10 also regulates PEX5 recycling by acting as a E3 ubiquitin-protein ligase. When PEX5 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 during its passage through the retrotranslocation channel, leading to its degradation.
Uniprot ID
O60683
Ensemble ID
ENST00000288774.8
HGNC ID
HGNC:8851

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL51 SNV: p.A37T .
KMS27 SNV: p.G23D .
SKMEL2 SNV: p.Y20H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C173(0.81)  LDD1507  [1]
TFBX
 Probe Info  		N.A.  LDD0027  [2]
IPM
 Probe Info  		N.A.  LDD0005  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C173(0.81)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C173(0.95)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C173(0.99)  LDD1509  [1]
 LDCM0270  AC15 HEK-293T C173(0.96)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C173(0.94)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C173(0.94)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C173(0.89)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C173(0.96)  LDD1518  [1]
 LDCM0281  AC21 HEK-293T C173(1.02)  LDD1520  [1]
 LDCM0283  AC23 HEK-293T C173(0.89)  LDD1522  [1]
 LDCM0284  AC24 HEK-293T C173(0.84)  LDD1523  [1]
 LDCM0285  AC25 HEK-293T C173(0.79)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C173(1.01)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C173(0.91)  LDD1526  [1]
 LDCM0289  AC29 HEK-293T C173(0.98)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C173(0.93)  LDD1529  [1]
 LDCM0292  AC31 HEK-293T C173(0.93)  LDD1531  [1]
 LDCM0293  AC32 HEK-293T C173(0.92)  LDD1532  [1]
 LDCM0294  AC33 HEK-293T C173(0.90)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C173(0.97)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C173(1.01)  LDD1535  [1]
 LDCM0298  AC37 HEK-293T C173(0.96)  LDD1537  [1]
 LDCM0300  AC39 HEK-293T C173(1.08)  LDD1539  [1]
 LDCM0302  AC40 HEK-293T C173(0.98)  LDD1541  [1]
 LDCM0303  AC41 HEK-293T C173(0.94)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C173(0.91)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C173(1.12)  LDD1544  [1]
 LDCM0307  AC45 HEK-293T C173(1.02)  LDD1546  [1]
 LDCM0309  AC47 HEK-293T C173(0.95)  LDD1548  [1]
 LDCM0310  AC48 HEK-293T C173(0.94)  LDD1549  [1]
 LDCM0311  AC49 HEK-293T C173(0.94)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C173(1.04)  LDD1551  [1]
 LDCM0313  AC50 HEK-293T C173(1.07)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C173(0.97)  LDD1553  [1]
 LDCM0316  AC53 HEK-293T C173(0.98)  LDD1555  [1]
 LDCM0318  AC55 HEK-293T C173(0.98)  LDD1557  [1]
 LDCM0319  AC56 HEK-293T C173(0.96)  LDD1558  [1]
 LDCM0320  AC57 HEK-293T C173(0.98)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C173(0.97)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C173(1.14)  LDD1561  [1]
 LDCM0325  AC61 HEK-293T C173(1.09)  LDD1564  [1]
 LDCM0327  AC63 HEK-293T C173(1.03)  LDD1566  [1]
 LDCM0328  AC64 HEK-293T C173(0.82)  LDD1567  [1]
 LDCM0334  AC7 HEK-293T C173(0.99)  LDD1568  [1]
 LDCM0345  AC8 HEK-293T C173(0.83)  LDD1569  [1]
 LDCM0248  AKOS034007472 HEK-293T C173(1.02)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C173(0.92)  LDD1570  [1]
 LDCM0275  AKOS034007705 HEK-293T C173(0.88)  LDD1514  [1]
 LDCM0367  CL1 HEK-293T C276(1.15)  LDD1571  [1]
 LDCM0370  CL101 HEK-293T C276(0.85)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C173(0.98)  LDD1575  [1]
 LDCM0374  CL105 HEK-293T C276(1.07)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C173(0.91)  LDD1579  [1]
 LDCM0378  CL109 HEK-293T C276(1.16)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C173(1.30)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C173(0.94)  LDD1584  [1]
 LDCM0383  CL113 HEK-293T C276(0.80)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C173(1.18)  LDD1588  [1]
 LDCM0387  CL117 HEK-293T C276(0.92)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C173(1.01)  LDD1592  [1]
 LDCM0390  CL12 HEK-293T C173(1.03)  LDD1594  [1]
 LDCM0392  CL121 HEK-293T C276(0.90)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C173(0.99)  LDD1597  [1]
 LDCM0396  CL125 HEK-293T C276(1.05)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C173(1.11)  LDD1601  [1]
 LDCM0400  CL13 HEK-293T C276(0.80)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C173(1.06)  LDD1605  [1]
 LDCM0404  CL17 HEK-293T C173(1.00)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C173(1.06)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C173(1.12)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C173(1.17)  LDD1611  [1]
 LDCM0409  CL21 HEK-293T C173(1.39)  LDD1613  [1]
 LDCM0411  CL23 HEK-293T C173(1.16)  LDD1615  [1]
 LDCM0412  CL24 HEK-293T C173(1.16)  LDD1616  [1]
 LDCM0413  CL25 HEK-293T C276(1.02)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C173(0.96)  LDD1618  [1]
 LDCM0417  CL29 HEK-293T C173(0.96)  LDD1621  [1]
 LDCM0419  CL30 HEK-293T C173(1.02)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C173(1.06)  LDD1624  [1]
 LDCM0422  CL33 HEK-293T C173(1.20)  LDD1626  [1]
 LDCM0424  CL35 HEK-293T C173(1.38)  LDD1628  [1]
 LDCM0425  CL36 HEK-293T C173(1.09)  LDD1629  [1]
 LDCM0426  CL37 HEK-293T C276(0.94)  LDD1630  [1]
 LDCM0431  CL41 HEK-293T C173(0.99)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C173(1.01)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C173(1.24)  LDD1637  [1]
 LDCM0435  CL45 HEK-293T C173(1.10)  LDD1639  [1]
 LDCM0437  CL47 HEK-293T C173(1.20)  LDD1641  [1]
 LDCM0438  CL48 HEK-293T C173(0.98)  LDD1642  [1]
 LDCM0439  CL49 HEK-293T C276(0.80)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C173(0.86)  LDD1644  [1]
 LDCM0441  CL50 HEK-293T C173(1.02)  LDD1645  [1]
 LDCM0444  CL53 HEK-293T C173(1.07)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C173(1.16)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C173(1.35)  LDD1649  [1]
 LDCM0448  CL57 HEK-293T C173(1.24)  LDD1651  [1]
 LDCM0450  CL59 HEK-293T C173(1.14)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C173(1.19)  LDD1654  [1]
 LDCM0452  CL60 HEK-293T C173(1.11)  LDD1655  [1]
 LDCM0453  CL61 HEK-293T C276(0.75)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C173(1.07)  LDD1657  [1]
 LDCM0457  CL65 HEK-293T C173(0.92)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C173(1.03)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C173(1.11)  LDD1662  [1]
 LDCM0461  CL69 HEK-293T C173(1.10)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C173(1.24)  LDD1665  [1]
 LDCM0464  CL71 HEK-293T C173(1.19)  LDD1667  [1]
 LDCM0465  CL72 HEK-293T C173(1.23)  LDD1668  [1]
 LDCM0466  CL73 HEK-293T C276(0.93)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C173(1.19)  LDD1670  [1]
 LDCM0470  CL77 HEK-293T C173(0.97)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C173(1.14)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C173(1.12)  LDD1675  [1]
 LDCM0475  CL81 HEK-293T C173(1.20)  LDD1678  [1]
 LDCM0477  CL83 HEK-293T C173(1.13)  LDD1680  [1]
 LDCM0478  CL84 HEK-293T C173(1.31)  LDD1681  [1]
 LDCM0479  CL85 HEK-293T C276(0.90)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C173(1.18)  LDD1683  [1]
 LDCM0483  CL89 HEK-293T C173(0.79)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C173(1.02)  LDD1687  [1]
 LDCM0485  CL90 HEK-293T C173(1.01)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C173(1.25)  LDD1689  [1]
 LDCM0488  CL93 HEK-293T C173(0.90)  LDD1691  [1]
 LDCM0490  CL95 HEK-293T C173(1.04)  LDD1693  [1]
 LDCM0491  CL96 HEK-293T C173(0.92)  LDD1694  [1]
 LDCM0492  CL97 HEK-293T C276(0.92)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C173(1.04)  LDD1696  [1]
 LDCM0427  Fragment51 HEK-293T C173(1.15)  LDD1631  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.