Details of the Target

General Information of Target

Target ID LDTP00650
Target Name Calpain-5 (CAPN5)
Gene Name CAPN5
Gene ID 726
Synonyms
NCL3; Calpain-5; EC 3.4.22.-; Calpain htra-3; New calpain 3; nCL-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFSCVKPYEDQNYSALRRDCRRRKVLFEDPLFPATDDSLYYKGTPGPAVRWKRPKGICED
PRLFVDGISSHDLHQGQVGNCWFVAACSSLASRESLWQKVIPDWKEQEWDPEKPNAYAGI
FHFHFWRFGEWVDVVIDDRLPTVNNQLIYCHSNSRNEFWCALVEKAYAKLAGCYQALDGG
NTADALVDFTGGVSEPIDLTEGDFANDETKRNQLFERMLKVHSRGGLISASIKAVTAADM
EARLACGLVKGHAYAVTDVRKVRLGHGLLAFFKSEKLDMIRLRNPWGEREWNGPWSDTSE
EWQKVSKSEREKMGVTVQDDGEFWMTFEDVCRYFTDIIKCRVINTSHLSIHKTWEEARLH
GAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGE
NLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHT
GEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYV
IIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKAD
PDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV
Target Bioclass
Enzyme
Family
Peptidase C2 family
Function Calcium-regulated non-lysosomal thiol-protease.
Uniprot ID
O15484
Ensemble ID
ENST00000529629.5
HGNC ID
HGNC:1482

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C246(1.02)  LDD3312  [1]
BTD
 Probe Info  		C246(0.83)  LDD2127  [2]
IPM
 Probe Info  		C246(1.61); C404(1.16)  LDD1702  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C377(1.48)  LDD1523  [5]
 LDCM0293  AC32 HEK-293T C377(1.23)  LDD1532  [5]
 LDCM0302  AC40 HEK-293T C377(0.94)  LDD1541  [5]
 LDCM0310  AC48 HEK-293T C377(1.33)  LDD1549  [5]
 LDCM0319  AC56 HEK-293T C377(1.18)  LDD1558  [5]
 LDCM0328  AC64 HEK-293T C377(1.12)  LDD1567  [5]
 LDCM0345  AC8 HEK-293T C377(1.09)  LDD1569  [5]
 LDCM0275  AKOS034007705 HEK-293T C377(1.08)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C377(1.05)  LDD1571  [5]
 LDCM0369  CL100 HEK-293T C377(1.00)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C377(0.91)  LDD1574  [5]
 LDCM0372  CL103 HEK-293T C246(1.09)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C377(0.99)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C377(0.99)  LDD1578  [5]
 LDCM0376  CL107 HEK-293T C246(1.06)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C377(1.11)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C377(0.92)  LDD1582  [5]
 LDCM0381  CL111 HEK-293T C246(0.92)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C377(0.91)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C377(1.22)  LDD1587  [5]
 LDCM0385  CL115 HEK-293T C246(1.03)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C377(0.97)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C377(0.90)  LDD1591  [5]
 LDCM0389  CL119 HEK-293T C246(1.20)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C377(1.06)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C377(0.98)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C377(1.13)  LDD1596  [5]
 LDCM0394  CL123 HEK-293T C246(1.22)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C377(0.94)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C377(0.97)  LDD1600  [5]
 LDCM0398  CL127 HEK-293T C246(0.99)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C377(0.97)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C377(0.84)  LDD1604  [5]
 LDCM0402  CL15 HEK-293T C246(1.38)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C377(0.96)  LDD1607  [5]
 LDCM0412  CL24 HEK-293T C377(1.50)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C377(0.96)  LDD1617  [5]
 LDCM0415  CL27 HEK-293T C246(0.91)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C377(1.05)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C246(1.06)  LDD1622  [5]
 LDCM0425  CL36 HEK-293T C377(1.24)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C377(0.93)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C246(1.03)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C377(1.03)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C377(1.08)  LDD1634  [5]
 LDCM0438  CL48 HEK-293T C377(1.08)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C377(0.95)  LDD1643  [5]
 LDCM0443  CL52 HEK-293T C377(1.03)  LDD1646  [5]
 LDCM0452  CL60 HEK-293T C377(1.28)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C377(0.77)  LDD1656  [5]
 LDCM0455  CL63 HEK-293T C246(1.03)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C377(0.90)  LDD1659  [5]
 LDCM0465  CL72 HEK-293T C377(0.99)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C377(0.81)  LDD1669  [5]
 LDCM0469  CL76 HEK-293T C377(0.89)  LDD1672  [5]
 LDCM0478  CL84 HEK-293T C377(1.04)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C377(0.93)  LDD1682  [5]
 LDCM0481  CL87 HEK-293T C246(0.89)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C377(1.04)  LDD1685  [5]
 LDCM0491  CL96 HEK-293T C377(1.35)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C377(0.88)  LDD1695  [5]
 LDCM0494  CL99 HEK-293T C246(1.02)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C246(1.05)  LDD1698  [5]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C246(1.61); C404(1.16)  LDD1702  [2]
 LDCM0468  Fragment33 HEK-293T C246(1.11)  LDD1671  [5]
 LDCM0022  KB02 42-MG-BA C377(1.50); C58(1.73)  LDD2244  [1]
 LDCM0023  KB03 42-MG-BA C377(1.48); C58(1.40)  LDD2661  [1]
 LDCM0024  KB05 HMCB C246(1.02)  LDD3312  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C246(0.83)  LDD2127  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402