Details of the Target

General Information of Target

Target ID LDTP00369
Target Name Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)
Gene Name GAPDHS
Gene ID 26330
Synonyms
GAPD2; GAPDH2; GAPDS; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; EC 1.2.1.12; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; GAPDH-2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPP
HPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKY
DSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAAS
DHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERF
GIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKL
TGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLG
DTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
Target Bioclass
Enzyme
Family
Glyceraldehyde-3-phosphate dehydrogenase family
Subcellular location
Cytoplasm
Function May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
Uniprot ID
O14556
Ensemble ID
ENST00000222286.9
HGNC ID
HGNC:24864

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C319(1.16)  LDD3413  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
Acrolein
 Probe Info  		N.A.  LDD0217  [5]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C319(0.83)  LDD1523  [7]
 LDCM0293  AC32 HEK-293T C319(1.59)  LDD1532  [7]
 LDCM0302  AC40 HEK-293T C319(1.25)  LDD1541  [7]
 LDCM0310  AC48 HEK-293T C319(0.92)  LDD1549  [7]
 LDCM0319  AC56 HEK-293T C319(1.36)  LDD1558  [7]
 LDCM0328  AC64 HEK-293T C319(1.27)  LDD1567  [7]
 LDCM0345  AC8 HEK-293T C319(1.21)  LDD1569  [7]
 LDCM0275  AKOS034007705 HEK-293T C319(1.13)  LDD1514  [7]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [5]
 LDCM0390  CL12 HEK-293T C319(0.97)  LDD1594  [7]
 LDCM0412  CL24 HEK-293T C319(1.05)  LDD1616  [7]
 LDCM0425  CL36 HEK-293T C319(0.87)  LDD1629  [7]
 LDCM0438  CL48 HEK-293T C319(1.32)  LDD1642  [7]
 LDCM0452  CL60 HEK-293T C319(1.48)  LDD1655  [7]
 LDCM0465  CL72 HEK-293T C319(1.18)  LDD1668  [7]
 LDCM0478  CL84 HEK-293T C319(1.13)  LDD1681  [7]
 LDCM0491  CL96 HEK-293T C319(1.71)  LDD1694  [7]
 LDCM0625  F8 Ramos C319(0.31)  LDD2187  [8]
 LDCM0572  Fragment10 Ramos C319(0.28)  LDD2189  [8]
 LDCM0573  Fragment11 Ramos C319(0.07)  LDD2190  [8]
 LDCM0574  Fragment12 Ramos C319(0.88)  LDD2191  [8]
 LDCM0575  Fragment13 Ramos C319(0.34)  LDD2192  [8]
 LDCM0576  Fragment14 Ramos C319(0.69)  LDD2193  [8]
 LDCM0579  Fragment20 Ramos C319(1.00)  LDD2194  [8]
 LDCM0580  Fragment21 Ramos C319(1.50)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C319(1.12)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C319(1.01)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C319(0.29)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C319(0.37)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C319(0.53)  LDD2200  [8]
 LDCM0590  Fragment32 Ramos C319(0.17)  LDD2201  [8]
 LDCM0468  Fragment33 Ramos C319(1.16)  LDD2202  [8]
 LDCM0596  Fragment38 Ramos C319(0.79)  LDD2203  [8]
 LDCM0566  Fragment4 Ramos C319(0.39)  LDD2184  [8]
 LDCM0610  Fragment52 Ramos C319(1.06)  LDD2204  [8]
 LDCM0614  Fragment56 Ramos C319(0.41)  LDD2205  [8]
 LDCM0569  Fragment7 Ramos C319(0.97)  LDD2186  [8]
 LDCM0571  Fragment9 Ramos C319(0.32)  LDD2188  [8]
 LDCM0022  KB02 Ramos C319(0.91)  LDD2182  [8]
 LDCM0023  KB03 Ramos C319(2.47)  LDD2183  [8]
 LDCM0024  KB05 RVH-421 C319(1.16)  LDD3413  [1]
 LDCM0109  NEM HeLa N.A.  LDD0223  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger MYND domain-containing protein 19 (ZMYND19) . Q96E35

The Drug(s) Related To This Target

Approved
Click To Hide/Show 6 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157
Artenimol . DB11638
Zinc . DB01593
Zinc Acetate . DB14487
Zinc Chloride . DB14533
Zinc Sulfate Unspecified Form . DB14548
Investigative
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
(+)-rutamarin Alcohol . DB02205
2'-deoxy-2'-[(35-dimethoxybenzoyl)Amino]-n-[(1r)-1234-tetrahydro-1-naphthalenyl]Adenosine . DB04477
N-naphthalen-1-ylmethyl-2'-[35-dimethoxybenzamido]-2'-deoxy-adenosine . DB03331

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578