Details of the Target

General Information of Target

Target ID LDTP00053
Target Name tRNA wybutosine-synthesizing protein 5 (TYW5)
Gene Name TYW5
Gene ID 129450
Synonyms
C2orf60; tRNA wybutosine-synthesizing protein 5; hTYW5; tRNA yW-synthesizing protein 5; EC 1.14.11.42; tRNA(Phe; 7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGQHLPVPRLEGVSREQFMQHLYPQRKPLVLEGIDLGPCTSKWTVDYLSQVGGKKEVKI
HVAAVAQMDFISKNFVYRTLPFDQLVQRAAEEKHKEFFVSEDEKYYLRSLGEDPRKDVAD
IRKQFPLLKGDIKFPEFFKEEQFFSSVFRISSPGLQLWTHYDVMDNLLIQVTGKKRVVLF
SPRDAQYLYLKGTKSEVLNIDNPDLAKYPLFSKARRYECSLEAGDVLFIPALWFHNVISE
EFGVGVNIFWKHLPSECYDKTDTYGNKDPTAASRAAQILDRALKTLAELPEEYRDFYARR
MVLHIQDKAYSKNSE
Target Bioclass
Enzyme
Family
TYW5 family
Function
tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.
Uniprot ID
A2RUC4
Ensemble ID
ENST00000354611.9
HGNC ID
HGNC:26754

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
G415 SNV: p.G54R .
HT115 SNV: p.R216K .
HUH1 SNV: p.K267N .
KMS27 SNV: p.S145F .
LS180 Deletion: p.K175SfsTer16 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C94(1.27)  LDD3325  [1]
NAIA_5
 Probe Info  		N.A.  LDD2223  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0259  AC14 HEK-293T C257(1.06)  LDD1512  [3]
 LDCM0282  AC22 HEK-293T C257(0.99)  LDD1521  [3]
 LDCM0284  AC24 HEK-293T C257(1.01)  LDD1523  [3]
 LDCM0291  AC30 HEK-293T C257(1.12)  LDD1530  [3]
 LDCM0293  AC32 HEK-293T C257(1.06)  LDD1532  [3]
 LDCM0299  AC38 HEK-293T C257(1.07)  LDD1538  [3]
 LDCM0302  AC40 HEK-293T C257(1.08)  LDD1541  [3]
 LDCM0308  AC46 HEK-293T C257(1.09)  LDD1547  [3]
 LDCM0310  AC48 HEK-293T C257(1.09)  LDD1549  [3]
 LDCM0317  AC54 HEK-293T C257(1.20)  LDD1556  [3]
 LDCM0319  AC56 HEK-293T C257(1.03)  LDD1558  [3]
 LDCM0323  AC6 HEK-293T C257(1.09)  LDD1562  [3]
 LDCM0326  AC62 HEK-293T C257(1.09)  LDD1565  [3]
 LDCM0328  AC64 HEK-293T C257(1.00)  LDD1567  [3]
 LDCM0345  AC8 HEK-293T C257(0.98)  LDD1569  [3]
 LDCM0275  AKOS034007705 HEK-293T C257(1.11)  LDD1514  [3]
 LDCM0368  CL10 HEK-293T C257(1.21)  LDD1572  [3]
 LDCM0390  CL12 HEK-293T C257(1.03)  LDD1594  [3]
 LDCM0410  CL22 HEK-293T C257(1.20)  LDD1614  [3]
 LDCM0412  CL24 HEK-293T C257(1.13)  LDD1616  [3]
 LDCM0423  CL34 HEK-293T C257(1.17)  LDD1627  [3]
 LDCM0425  CL36 HEK-293T C257(1.09)  LDD1629  [3]
 LDCM0436  CL46 HEK-293T C257(1.13)  LDD1640  [3]
 LDCM0438  CL48 HEK-293T C257(0.98)  LDD1642  [3]
 LDCM0449  CL58 HEK-293T C257(1.28)  LDD1652  [3]
 LDCM0452  CL60 HEK-293T C257(1.01)  LDD1655  [3]
 LDCM0463  CL70 HEK-293T C257(1.26)  LDD1666  [3]
 LDCM0465  CL72 HEK-293T C257(1.04)  LDD1668  [3]
 LDCM0476  CL82 HEK-293T C257(1.13)  LDD1679  [3]
 LDCM0478  CL84 HEK-293T C257(1.08)  LDD1681  [3]
 LDCM0489  CL94 HEK-293T C257(1.13)  LDD1692  [3]
 LDCM0491  CL96 HEK-293T C257(1.03)  LDD1694  [3]
 LDCM0022  KB02 786-O C94(1.17)  LDD2247  [1]
 LDCM0023  KB03 786-O C94(1.44)  LDD2664  [1]
 LDCM0024  KB05 UACC257 C94(1.27)  LDD3325  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402