Details of the Target

General Information of Target

Target ID LDTP14286
Target Name AP-1 complex subunit mu-2 (AP1M2)
Gene Name AP1M2
Gene ID 10053
Synonyms
AP-1 complex subunit mu-2; AP-mu chain family member mu1B; Adaptor protein complex AP-1 subunit mu-2; Adaptor-related protein complex 1 subunit mu-2; Clathrin assembly protein complex 1 mu-2 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Mu-adaptin 2; Mu1B-adaptin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MCSTNPGKWVTFDDDPAVQSSQKSKNFPLENQGVCRPNGLKLNLPGLREFPSGSSSTSST
PLSSPIVDFYFSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPESSSDSPLAISGGE
SSLLPTRPTCLSHALLPSDHSCTHPTPKVGLPDEVNPQQAESLGFQSDDLPQFQYFREDC
AFSSPFWKDEGSDSHFTLDPPGSKKMFSSRNKEMPIDQKSLNKCSLNYICEKLEHLQSAE
NQDSLRSLSMHCLCAEENASSFVPHTLFRSQPKSGWSFMLRIPEKKNMMSSRQWGPIFLK
VLPGGILQMYYEQGLEKPFKEIQLDPYCRLSEPKVENFSVAGKIHTVKIEHVSYTEKRKY
HSKTEVVHEPDIEQMLKLGSTSYHDFLDFLTTVEEELMKLPAVSKPKKNYEEQEISLEIV
DNFWGKVTKEGKFVESAVITQIYCLCFVNGNLECFLTLNDLELPKRDESYYEKDSEKKGI
DILDYHFHKCVNVQEFEQSRIIKFVPLDACRFELMRFKTLYNGDNLPFSLKSVVVVQGAY
VELQAFVNMASLAQRSSYAGSLRSCDNIRIHFPVPSQWIKALWTMNLQRQKSLKAKMNRR
ACLGSLQELESEPVIQVTVGSAKYESAYQAVVWKIDRLPDKNSSLDHPHCLSYKLELGSD
QEIPSDWYPFATVQFSVPDTCASRTEVRSLGVESDVQPQKHVQQRACYNIQVEIEKKWIK
IDGEDPDKIGDCITQ
Target Bioclass
Other
Family
Adaptor complexes medium subunit family
Subcellular location
Golgi apparatus
Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Uniprot ID
Q9Y6Q5
Ensemble ID
ENST00000250244.11
HGNC ID
HGNC:558

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC70 SNV: p.N339S .
HT115 SNV: p.K227N .
IM95 SNV: p.R104W DBIA    Probe Info 
LN18 SNV: p.E93K DBIA    Probe Info 
MOLT4 SNV: p.E311D IA-alkyne    Probe Info 
PEO1 SNV: p.R251G .
SKMEL24 SNV: p.E42Ter DBIA    Probe Info 
TOV21G SNV: p.P123Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C253(2.84)  LDD3310  [1]
BTD
 Probe Info  		C241(1.59)  LDD2092  [2]
IPM
 Probe Info  		C241(1.18)  LDD1702  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C241(0.44)  LDD2095  [2]
 LDCM0025  4SU-RNA HEK-293T C241(20.00)  LDD0371  [4]
 LDCM0026  4SU-RNA+native RNA HEK-293T C241(9.74)  LDD0372  [4]
 LDCM0545  Acetamide MDA-MB-231 C241(0.63)  LDD2138  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C241(1.18)  LDD1702  [2]
 LDCM0022  KB02 22RV1 C92(1.82)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C92(3.28)  LDD2660  [1]
 LDCM0024  KB05 COLO792 C253(2.84)  LDD3310  [1]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C241(1.59)  LDD2092  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C241(1.17)  LDD2093  [2]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C241(1.89)  LDD2094  [2]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C241(0.87)  LDD2098  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C241(0.88)  LDD2101  [2]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C241(0.11)  LDD2116  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C241(0.87)  LDD2128  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C241(0.81)  LDD2133  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C241(0.27)  LDD2141  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C241(0.41)  LDD2148  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AP-2 complex subunit beta (AP2B1) Adaptor complexes large subunit family P63010

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625