Details of the Target

General Information of Target

Target ID LDTP13999
Target Name Signaling threshold-regulating transmembrane adapter 1 (SIT1)
Gene Name SIT1
Gene ID 27240
Synonyms
SIT; Signaling threshold-regulating transmembrane adapter 1; SHP2-interacting transmembrane adapter protein; Suppression-inducing transmembrane adapter 1; gp30/40
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGPQGARRQAFLAFGDVTVDFTQKEWRLLSPAQRALYREVTLENYSHLVSLGILHSKPEL
IRRLEQGEVPWGEERRRRPGPCAGIYAEHVLRPKNLGLAHQRQQQLQFSDQSFQSDTAEG
QEKEKSTKPMAFSSPPLRHAVSSRRRNSVVEIESSQGQRENPTEIDKVLKGIENSRWGAF
KCAERGQDFSRKMMVIIHKKAHSRQKLFTCRECHQGFRDESALLLHQNTHTGEKSYVCSV
CGRGFSLKANLLRHQRTHSGEKPFLCKVCGRGYTSKSYLTVHERTHTGEKPYECQECGRR
FNDKSSYNKHLKAHSGEKPFVCKECGRGYTNKSYFVVHKRIHSGEKPYRCQECGRGFSNK
SHLITHQRTHSGEKPFACRQCKQSFSVKGSLLRHQRTHSGEKPFVCKDCERSFSQKSTLV
YHQRTHSGEKPFVCRECGQGFIQKSTLVKHQITHSEEKPFVCKDCGRGFIQKSTFTLHQR
THSEEKPYGCRECGRRFRDKSSYNKHLRAHLGEKRFFCRDCGRGFTLKPNLTIHQRTHSG
EKPFMCKQCEKSFSLKANLLRHQWTHSGERPFNCKDCGRGFILKSTLLFHQKTHSGEKPF
ICSECGQGFIWKSNLVKHQLAHSGKQPFVCKECGRGFNWKGNLLTHQRTHSGEKPFVCNV
CGQGFSWKRSLTRHHWRIHSKEKPFVCQECKRGYTSKSDLTVHERIHTGERPYECQECGR
KFSNKSYYSKHLKRHLREKRFCTGSVGEASS
Target Bioclass
Other
Subcellular location
Cell membrane
Function Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.
Uniprot ID
Q9Y3P8
Ensemble ID
ENST00000259608.8
HGNC ID
HGNC:17710

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TG42
 Probe Info  		5.66  LDD0043  [1]
DBIA
 Probe Info  		C173(4.02)  LDD0209  [2]
IA-alkyne
 Probe Info  		C173(5.59)  LDD1710  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C173(1.89); C126(1.09)  LDD2187  [4]
 LDCM0573  Fragment11 Ramos C126(0.87)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C173(1.12)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C173(2.21)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C173(5.97); C126(1.16)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C173(1.74)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C173(0.95)  LDD2195  [4]
 LDCM0578  Fragment27 Ramos C173(1.56)  LDD2197  [4]
 LDCM0588  Fragment30 Ramos C173(2.46)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C173(1.64)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C173(3.19)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C173(2.28)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C173(1.00)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C173(1.42); C126(1.12)  LDD2184  [4]
 LDCM0569  Fragment7 Ramos C173(1.96); C126(0.80)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C173(1.87)  LDD2188  [4]
 LDCM0022  KB02 Ramos C126(1.04)  LDD2182  [4]
 LDCM0023  KB03 Jurkat C173(4.02)  LDD0209  [2]
 LDCM0024  KB05 T cell-activated C173(5.59)  LDD1710  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bax inhibitor 1 (TMBIM6) BI1 family P55061
Clarin-2 (CLRN2) Clarin family A0PK11
Integral membrane protein 2A (ITM2A) ITM2 family O43736
Cardiac phospholamban (PLN) Phospholamban family P26678
Tetraspanin-33 (TSPAN33) Tetraspanin (TM4SF) family Q86UF1
Uroplakin-1b (UPK1B) Tetraspanin (TM4SF) family O75841
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Apolipoprotein L2 (APOL2) Apolipoprotein L family Q9BQE5
MAL-like protein (MALL) MAL family Q13021
Bone morphogenetic protein 10 (BMP10) TGF-beta family O95393
Transmembrane protein 140 (TMEM140) . Q9NV12

References

1 Design and synthesis of tailored human caseinolytic protease P inhibitors. Chem Commun (Camb). 2018 Aug 28;54(70):9833-9836. doi: 10.1039/c8cc05265d.
Mass spectrometry data entry: PXD010277
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578