Details of the Target

General Information of Target

Target ID LDTP13899
Target Name Phospholipid-transporting ATPase IA (ATP8A1)
Gene Name ATP8A1
Gene ID 10396
Synonyms
ATPIA; Phospholipid-transporting ATPase IA; EC 7.6.2.1; ATPase class I type 8A member 1; Chromaffin granule ATPase II; P4-ATPase flippase complex alpha subunit ATP8A1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATQAHSLSYAGCNFLRQRLVLSTLSGRPVKIRKIRARDDNPGLRDFEASFIRLLDKITN
GSRIEINQTGTTLYYQPGLLYGGSVEHDCSVLRGIGYYLESLLCLAPFMKHPLKIVLRGV
TNDQVDPSVDVLKATALPLLKQFGIDGESFELKIVRRGMPPGGGGEVVFSCPVRKVLKPI
QLTDPGKIKRIRGMAYSVRVSPQMANRIVDSARSILNKFIPDIYIYTDHMKGVNSGKSPG
FGLSLVAETTSGTFLSAELASNPQGQGAAVLPEDLGRNCARLLLEEIYRGGCVDSTNQSL
ALLLMTLGQQDVSKVLLGPLSPYTIEFLRHLKSFFQIMFKIETKPCGEELKGGDKVLMTC
VGIGFSNLSKTLK
Target Bioclass
Enzyme
Family
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
Subcellular location
Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane
Function
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the cell membrane. Acts as aminophospholipid translocase at the cell membrane in neuronal cells.
Uniprot ID
Q9Y2Q0
Ensemble ID
ENST00000264449.14
HGNC ID
HGNC:13531

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAMA1 SNV: p.Q627Ter .
CCK81 SNV: p.T3A .
COLO792 SNV: p.H1133Y .
G361 Substitution: p.D763N .
HCT15 SNV: p.D665Y; p.I818V .
HT115 SNV: p.D460G; p.V855A .
IGR1 SNV: p.G966S .
MFE319 Deletion: p.S1102AfsTer2 .
NCIH146 SNV: p.L646F .
NCIH661 SNV: p.D127H .
RAMOS SNV: p.H488Q .
TE1 SNV: p.V1050A .
TE4 Deletion: p.L547WfsTer12 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1111(6.67)  LDD0277  [1]
DBIA
 Probe Info  		C416(1.75)  LDD3372  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C699(1.03); C727(1.03)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C727(0.97)  LDD1509  [4]
 LDCM0237  AC12 HEK-293T C699(1.06)  LDD1510  [4]
 LDCM0270  AC15 HEK-293T C699(1.25)  LDD1513  [4]
 LDCM0277  AC18 HEK-293T C699(1.07); C727(1.06)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C727(0.96)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C699(1.05); C727(1.05)  LDD1518  [4]
 LDCM0280  AC20 HEK-293T C699(0.94)  LDD1519  [4]
 LDCM0283  AC23 HEK-293T C699(0.91)  LDD1522  [4]
 LDCM0286  AC26 HEK-293T C699(1.03); C727(1.02)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C727(0.99)  LDD1526  [4]
 LDCM0288  AC28 HEK-293T C699(0.92)  LDD1527  [4]
 LDCM0290  AC3 HEK-293T C727(0.91)  LDD1529  [4]
 LDCM0292  AC31 HEK-293T C699(0.95)  LDD1531  [4]
 LDCM0295  AC34 HEK-293T C699(1.07); C727(1.03)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C727(0.93)  LDD1535  [4]
 LDCM0297  AC36 HEK-293T C699(0.96)  LDD1536  [4]
 LDCM0300  AC39 HEK-293T C699(1.16)  LDD1539  [4]
 LDCM0301  AC4 HEK-293T C699(1.00)  LDD1540  [4]
 LDCM0304  AC42 HEK-293T C699(1.22); C727(0.95)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C727(0.99)  LDD1544  [4]
 LDCM0306  AC44 HEK-293T C699(0.91)  LDD1545  [4]
 LDCM0309  AC47 HEK-293T C699(1.16)  LDD1548  [4]
 LDCM0313  AC50 HEK-293T C699(1.08); C727(0.85)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C727(0.99)  LDD1553  [4]
 LDCM0315  AC52 HEK-293T C699(0.93)  LDD1554  [4]
 LDCM0318  AC55 HEK-293T C699(1.11)  LDD1557  [4]
 LDCM0321  AC58 HEK-293T C699(1.09); C727(0.94)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C727(0.96)  LDD1561  [4]
 LDCM0324  AC60 HEK-293T C699(0.91)  LDD1563  [4]
 LDCM0327  AC63 HEK-293T C699(1.24)  LDD1566  [4]
 LDCM0334  AC7 HEK-293T C699(0.87)  LDD1568  [4]
 LDCM0371  CL102 HEK-293T C699(0.99)  LDD1575  [4]
 LDCM0375  CL106 HEK-293T C699(0.92)  LDD1579  [4]
 LDCM0379  CL11 HEK-293T C699(1.30)  LDD1583  [4]
 LDCM0380  CL110 HEK-293T C699(1.01)  LDD1584  [4]
 LDCM0384  CL114 HEK-293T C699(0.93)  LDD1588  [4]
 LDCM0388  CL118 HEK-293T C699(0.89)  LDD1592  [4]
 LDCM0393  CL122 HEK-293T C699(0.91)  LDD1597  [4]
 LDCM0397  CL126 HEK-293T C699(0.98)  LDD1601  [4]
 LDCM0401  CL14 HEK-293T C699(0.96)  LDD1605  [4]
 LDCM0405  CL18 HEK-293T C699(1.22); C727(1.32)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C727(1.02)  LDD1610  [4]
 LDCM0407  CL2 HEK-293T C699(0.85)  LDD1611  [4]
 LDCM0408  CL20 HEK-293T C699(1.11)  LDD1612  [4]
 LDCM0411  CL23 HEK-293T C699(1.23)  LDD1615  [4]
 LDCM0414  CL26 HEK-293T C699(0.83)  LDD1618  [4]
 LDCM0419  CL30 HEK-293T C699(1.44); C727(1.21)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C727(1.01)  LDD1624  [4]
 LDCM0421  CL32 HEK-293T C699(1.05)  LDD1625  [4]
 LDCM0424  CL35 HEK-293T C699(0.90)  LDD1628  [4]
 LDCM0432  CL42 HEK-293T C699(1.15); C727(0.96)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C727(1.10)  LDD1637  [4]
 LDCM0434  CL44 HEK-293T C699(1.05)  LDD1638  [4]
 LDCM0437  CL47 HEK-293T C699(0.76)  LDD1641  [4]
 LDCM0441  CL50 HEK-293T C699(0.91)  LDD1645  [4]
 LDCM0445  CL54 HEK-293T C699(1.25); C727(1.15)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C727(1.12)  LDD1649  [4]
 LDCM0447  CL56 HEK-293T C699(1.20)  LDD1650  [4]
 LDCM0450  CL59 HEK-293T C699(0.96)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C699(1.66); C727(1.21)  LDD1654  [4]
 LDCM0454  CL62 HEK-293T C699(0.83)  LDD1657  [4]
 LDCM0458  CL66 HEK-293T C699(1.11); C727(0.93)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C727(1.10)  LDD1662  [4]
 LDCM0460  CL68 HEK-293T C699(1.03)  LDD1663  [4]
 LDCM0462  CL7 HEK-293T C727(1.02)  LDD1665  [4]
 LDCM0464  CL71 HEK-293T C699(0.98)  LDD1667  [4]
 LDCM0467  CL74 HEK-293T C699(0.84)  LDD1670  [4]
 LDCM0471  CL78 HEK-293T C699(1.07); C727(1.17)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C727(0.92)  LDD1675  [4]
 LDCM0473  CL8 HEK-293T C699(1.02)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C699(0.97)  LDD1677  [4]
 LDCM0477  CL83 HEK-293T C699(1.08)  LDD1680  [4]
 LDCM0480  CL86 HEK-293T C699(0.91)  LDD1683  [4]
 LDCM0485  CL90 HEK-293T C699(2.23); C727(1.15)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C727(0.74)  LDD1689  [4]
 LDCM0487  CL92 HEK-293T C699(1.12)  LDD1690  [4]
 LDCM0490  CL95 HEK-293T C699(1.99)  LDD1693  [4]
 LDCM0493  CL98 HEK-293T C699(0.93)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C699(0.88)  LDD1631  [4]
 LDCM0022  KB02 HG-3 C416(1.35)  LDD2355  [2]
 LDCM0023  KB03 HG-3 C416(1.34)  LDD2772  [2]
 LDCM0024  KB05 OCI-Ly3 C416(1.75)  LDD3372  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell cycle control protein 50A (TMEM30A) CDC50/LEM3 family Q9NV96

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Phosphatidyl Serine Small molecular drug DB00144

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402