General Information of Target

Target ID LDTP13680
Target Name RNA-binding protein Nova-2 (NOVA2)
Gene Name NOVA2
Gene ID 4858
Synonyms
ANOVA; NOVA3; RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPAN
CLTAWLALLQVLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAY
IFFCNIYVSILFLCCISCDRFVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQT
EDKETCFDMLQMDSRIAGYYYARFTVGFAIPLSIIAFTNHRIFRSIKQSMGLSAAQKAKV
KHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMCGLEERLYTASVVFLCLSTVN
GVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFS
RPVHPPGSPCPAKRLIEESC
Target Bioclass
Other
Subcellular location
Nucleus
Function
Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Uniquely regulates alternative splicing events of a series of axon guidance related genes during cortical development, being essential for central nervous system development by regulating neural networks wiring. Regulates differentially alternative splicing on the same transcripts expressed in different neurons. This includes functional differences in transcripts expressed in cortical and cerebellar excitatory versus inhibitory neurons where is required for, respectively, development of laminar structure and motor coordination and synapse formation. Also the regulation the regulation of intron retention can sequester the trans-acting splicing factor PTBP2, acting as a variable cis-acting scaffolding platform for PTBP2 across various natural conditions.
Uniprot ID
Q9UNW9
Ensemble ID
ENST00000263257.6
HGNC ID
HGNC:7887

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info 
C21(0.99)  LDD1510  [1]
IPM
 Probe Info 
N.A.  LDD2156  [2]
Phosphinate-6
 Probe Info 
N.A.  LDD0018  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C21(0.99)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C21(0.95)  LDD1512  [1]
 LDCM0280  AC20 HEK-293T C21(1.00)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C21(0.99)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C21(0.97)  LDD1521  [1]
 LDCM0288  AC28 HEK-293T C21(0.95)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C21(0.93)  LDD1528  [1]
 LDCM0291  AC30 HEK-293T C21(1.06)  LDD1530  [1]
 LDCM0297  AC36 HEK-293T C21(0.95)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C21(0.96)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C21(1.13)  LDD1538  [1]
 LDCM0301  AC4 HEK-293T C21(1.01)  LDD1540  [1]
 LDCM0306  AC44 HEK-293T C21(1.02)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C21(1.04)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C21(1.28)  LDD1547  [1]
 LDCM0312  AC5 HEK-293T C21(0.90)  LDD1551  [1]
 LDCM0315  AC52 HEK-293T C21(1.01)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C21(0.97)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C21(1.05)  LDD1556  [1]
 LDCM0323  AC6 HEK-293T C21(1.08)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C21(1.03)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C21(0.97)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C21(1.14)  LDD1565  [1]
 LDCM0248  AKOS034007472 HEK-293T C21(0.98)  LDD1511  [1]
 LDCM0368  CL10 HEK-293T C21(1.43)  LDD1572  [1]
 LDCM0371  CL102 HEK-293T C21(1.13)  LDD1575  [1]
 LDCM0375  CL106 HEK-293T C21(0.99)  LDD1579  [1]
 LDCM0380  CL110 HEK-293T C21(0.96)  LDD1584  [1]
 LDCM0384  CL114 HEK-293T C21(1.06)  LDD1588  [1]
 LDCM0388  CL118 HEK-293T C21(0.98)  LDD1592  [1]
 LDCM0393  CL122 HEK-293T C21(1.06)  LDD1597  [1]
 LDCM0397  CL126 HEK-293T C21(1.01)  LDD1601  [1]
 LDCM0401  CL14 HEK-293T C21(0.98)  LDD1605  [1]
 LDCM0407  CL2 HEK-293T C21(1.07)  LDD1611  [1]
 LDCM0408  CL20 HEK-293T C21(1.11)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C21(1.18)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C21(1.37)  LDD1614  [1]
 LDCM0414  CL26 HEK-293T C21(0.94)  LDD1618  [1]
 LDCM0421  CL32 HEK-293T C21(1.11)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C21(1.19)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C21(1.31)  LDD1627  [1]
 LDCM0434  CL44 HEK-293T C21(0.93)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C21(1.29)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C21(1.35)  LDD1640  [1]
 LDCM0441  CL50 HEK-293T C21(1.05)  LDD1645  [1]
 LDCM0447  CL56 HEK-293T C21(1.01)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C21(1.20)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C21(1.33)  LDD1652  [1]
 LDCM0454  CL62 HEK-293T C21(0.96)  LDD1657  [1]
 LDCM0460  CL68 HEK-293T C21(0.94)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C21(1.09)  LDD1664  [1]
 LDCM0463  CL70 HEK-293T C21(1.16)  LDD1666  [1]
 LDCM0467  CL74 HEK-293T C21(0.99)  LDD1670  [1]
 LDCM0473  CL8 HEK-293T C21(1.33)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C21(1.07)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C21(1.06)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C21(1.21)  LDD1679  [1]
 LDCM0480  CL86 HEK-293T C21(1.12)  LDD1683  [1]
 LDCM0484  CL9 HEK-293T C21(1.26)  LDD1687  [1]
 LDCM0487  CL92 HEK-293T C21(1.11)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C21(1.01)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C21(1.31)  LDD1692  [1]
 LDCM0493  CL98 HEK-293T C21(1.00)  LDD1696  [1]
 LDCM0427  Fragment51 HEK-293T C21(0.98)  LDD1631  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004