Details of the Target

General Information of Target

Target ID LDTP13433
Target Name Ubiquitin carboxyl-terminal hydrolase 21 (USP21)
Gene Name USP21
Gene ID 27005
Synonyms
USP23; Ubiquitin carboxyl-terminal hydrolase 21; EC 3.4.19.12; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTTTTTFKGVDPNSRNSSRVLRPPGGGSNFSLGFDEPTEQPVRKNKMASNIFGTPEENQA
SWAKSAGAKSSGGREDLESSGLQRRNSSEASSGDFLDLKGEGDIHENVDTDLPGSLGQSE
EKPVPAAPVPSPVAPAPVPSRRNPPGGKSSLVLG
Target Bioclass
Enzyme
Family
Peptidase C19 family, USP21 subfamily
Subcellular location
Cytoplasm
Function
Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Deubiquitinates BAZ2A/TIP5 leading to its stabilization. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Also acts as a negative regulator of the ribosome quality control (RQC) by mediating deubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS20/uS10, thereby antagonizing ZNF598-mediated 40S ubiquitination.
Uniprot ID
Q9UK80
Ensemble ID
ENST00000289865.12
HGNC ID
HGNC:12620
ChEMBL ID
CHEMBL2157852

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 Deletion: p.G412AfsTer18 .
JURKAT SNV: p.R319H .
MCAS SNV: p.P21T; p.R486Ter .
MOLT4 SNV: p.E427Ter .
NCIH1793 SNV: p.R234P .
SKMEL24 SNV: p.V396A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C419(0.88)  LDD1492  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C419(1.21)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C419(1.03)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C419(1.06)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C419(1.18)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C419(0.83); C384(1.25)  LDD1512  [1]
 LDCM0276  AC17 HEK-293T C419(1.10)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C419(1.01)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C419(1.04)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C419(0.88)  LDD1518  [1]
 LDCM0280  AC20 HEK-293T C419(1.08)  LDD1519  [1]
 LDCM0282  AC22 HEK-293T C419(1.24); C384(1.01)  LDD1521  [1]
 LDCM0285  AC25 HEK-293T C419(1.30)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C419(1.19)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C419(0.97)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C419(1.04)  LDD1527  [1]
 LDCM0290  AC3 HEK-293T C419(1.13)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C419(1.01); C384(0.79)  LDD1530  [1]
 LDCM0294  AC33 HEK-293T C419(1.09)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C419(1.11)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C419(1.26)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C419(1.05)  LDD1536  [1]
 LDCM0299  AC38 HEK-293T C419(1.01); C384(1.13)  LDD1538  [1]
 LDCM0301  AC4 HEK-293T C419(1.28)  LDD1540  [1]
 LDCM0303  AC41 HEK-293T C419(0.91)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C419(1.25)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C419(1.09)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C419(1.18)  LDD1545  [1]
 LDCM0308  AC46 HEK-293T C419(1.30); C384(0.95)  LDD1547  [1]
 LDCM0311  AC49 HEK-293T C419(0.98)  LDD1550  [1]
 LDCM0313  AC50 HEK-293T C419(1.19)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C419(1.23)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C419(1.02)  LDD1554  [1]
 LDCM0317  AC54 HEK-293T C419(0.99); C384(1.07)  LDD1556  [1]
 LDCM0320  AC57 HEK-293T C419(1.11)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C419(0.88)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C419(1.07)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C419(1.14); C384(0.87)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C419(0.88)  LDD1563  [1]
 LDCM0326  AC62 HEK-293T C419(1.00); C384(1.02)  LDD1565  [1]
 LDCM0356  AKOS034007680 HEK-293T C419(1.15)  LDD1570  [1]
 LDCM0367  CL1 HEK-293T C384(0.54)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C419(0.81); C384(1.76)  LDD1572  [1]
 LDCM0370  CL101 HEK-293T C384(0.48)  LDD1574  [1]
 LDCM0374  CL105 HEK-293T C384(0.62)  LDD1578  [1]
 LDCM0378  CL109 HEK-293T C384(0.44)  LDD1582  [1]
 LDCM0383  CL113 HEK-293T C384(0.63)  LDD1587  [1]
 LDCM0387  CL117 HEK-293T C384(0.44)  LDD1591  [1]
 LDCM0392  CL121 HEK-293T C384(0.53)  LDD1596  [1]
 LDCM0396  CL125 HEK-293T C384(0.50)  LDD1600  [1]
 LDCM0400  CL13 HEK-293T C384(0.55)  LDD1604  [1]
 LDCM0404  CL17 HEK-293T C419(0.83)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C419(0.93)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C419(1.08)  LDD1610  [1]
 LDCM0408  CL20 HEK-293T C419(1.24)  LDD1612  [1]
 LDCM0410  CL22 HEK-293T C419(0.79); C384(3.07)  LDD1614  [1]
 LDCM0413  CL25 HEK-293T C384(0.45)  LDD1617  [1]
 LDCM0417  CL29 HEK-293T C419(1.11)  LDD1621  [1]
 LDCM0419  CL30 HEK-293T C419(1.04)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C419(0.77)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C419(0.93)  LDD1625  [1]
 LDCM0423  CL34 HEK-293T C419(0.96); C384(2.30)  LDD1627  [1]
 LDCM0426  CL37 HEK-293T C384(0.42)  LDD1630  [1]
 LDCM0431  CL41 HEK-293T C419(0.95)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C419(0.87)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C419(0.81)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C419(0.66)  LDD1638  [1]
 LDCM0436  CL46 HEK-293T C419(0.86); C384(1.70)  LDD1640  [1]
 LDCM0439  CL49 HEK-293T C384(0.50)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C419(0.87)  LDD1644  [1]
 LDCM0444  CL53 HEK-293T C419(0.81)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C419(0.76)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C419(0.96)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C419(0.84)  LDD1650  [1]
 LDCM0449  CL58 HEK-293T C419(1.21); C384(2.31)  LDD1652  [1]
 LDCM0451  CL6 HEK-293T C419(0.95)  LDD1654  [1]
 LDCM0453  CL61 HEK-293T C384(0.52)  LDD1656  [1]
 LDCM0457  CL65 HEK-293T C419(1.01)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C419(1.12)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C419(0.86)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C419(0.86)  LDD1663  [1]
 LDCM0462  CL7 HEK-293T C419(0.75)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C419(1.04); C384(2.75)  LDD1666  [1]
 LDCM0466  CL73 HEK-293T C384(0.52)  LDD1669  [1]
 LDCM0470  CL77 HEK-293T C419(0.53)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C419(1.14)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C419(0.91)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C419(0.77)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C419(1.10)  LDD1677  [1]
 LDCM0476  CL82 HEK-293T C419(1.21); C384(1.94)  LDD1679  [1]
 LDCM0479  CL85 HEK-293T C384(0.39)  LDD1682  [1]
 LDCM0483  CL89 HEK-293T C419(1.20)  LDD1686  [1]
 LDCM0485  CL90 HEK-293T C419(0.92)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C419(0.93)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C419(0.84)  LDD1690  [1]
 LDCM0489  CL94 HEK-293T C419(0.98); C384(1.97)  LDD1692  [1]
 LDCM0492  CL97 HEK-293T C384(0.79)  LDD1695  [1]
 LDCM0022  KB02 HEK-293T C419(0.88)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C419(1.01)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C419(1.04)  LDD1502  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) Peptidase C12 family P09936
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-C10 (HOXC10) Abd-B homeobox family Q9NYD6
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) Fibulin family O95967
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
BTB/POZ domain-containing protein KCTD9 (KCTD9) . Q7L273

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402