Details of the Target

General Information of Target

Target ID LDTP13312
Target Name Adhesion G protein-coupled receptor E2 (ADGRE2)
Gene Name ADGRE2
Gene ID 30817
Synonyms
EMR2; Adhesion G protein-coupled receptor E2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; CD antigen CD312
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLT
PEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQ
RQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVP
EAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDD
DIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVG
KAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTL
AQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKE
EEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLE
GEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGR
KVVAEVYDQERFDNSDLSA
Target Bioclass
GPCR
Family
G-protein coupled receptor 2 family, Adhesion G-protein coupled receptor (ADGR) subfamily
Subcellular location
Cell membrane
Function
Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation.
Uniprot ID
Q9UHX3
Ensemble ID
ENST00000315576.8
HGNC ID
HGNC:3337

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL78 Substitution: p.L160A .
ETK1 SNV: p.L553M .
HCT116 SNV: p.D224G .
HEC1B SNV: p.G398C IA-alkyne    Probe Info 
IGROV1 SNV: p.H607R .
JURKAT SNV: p.S509N .
MCC13 SNV: p.P655L .
MFE319 SNV: p.G398C .
SNU1 SNV: p.N206S .
SSP25 SNV: p.L553M .
TFK1 SNV: p.C161G .
WM1552C SNV: p.V659L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info  		10.00  LDD0448  [1]
DBIA
 Probe Info  		C328(1.22)  LDD3397  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 BT-549 C328(1.42)  LDD2279  [2]
 LDCM0023  KB03 DEL C328(4.57)  LDD2732  [2]
 LDCM0024  KB05 PF-382 C328(1.22)  LDD3397  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Very long chain fatty acid elongase 4 (ELOVL4) ELO family Q9GZR5
Very long chain fatty acid elongase 7 (ELOVL7) ELO family A1L3X0
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
GTP-binding protein SAR1a (SAR1A) SAR1 family Q9NR31
TLC domain-containing protein 4 (TLCD4) TLCD4 family Q96MV1
E3 ubiquitin-protein ligase MARCHF6 (MARCHF6) . O60337
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Solute carrier family 7 member 14 (SLC7A14) Cationic amino acid transporter (CAT) (TC 2.A.3.3) family Q8TBB6
Molybdate-anion transporter (MFSD5) Major facilitator superfamily Q6N075
ER membrane protein complex subunit 5 (MMGT1) Membrane magnesium transporter (TC 1.A.67) family Q8N4V1
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Killer cell immunoglobulin-like receptor 2DL3 (KIR2DL3) Immunoglobulin superfamily P43628
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Transcription termination factor 3, mitochondrial (MTERF3) MTERF family Q96E29
Bcl-2-interacting killer (BIK) . Q13323
Coiled-coil domain-containing protein 107 (CCDC107) . Q8WV48
Complement factor H-related protein 1 (CFHR1) . Q03591
Transmembrane protein 80 (TMEM80) . Q96HE8

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060