Details of the Target

General Information of Target

Target ID LDTP13238
Target Name Tryptophan--tRNA ligase, mitochondrial (WARS2)
Gene Name WARS2
Gene ID 10352
Synonyms
Tryptophan--tRNA ligase, mitochondrial; EC 6.1.1.2; (Mt)TrpRS; Tryptophanyl-tRNA synthetase; TrpRS
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGLLLPLALCILVLCCGAMSPPQLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGY
VLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAI
FYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYNN
ENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGS
LTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGP
RGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPK
EKARTAECPGPAQNASPLVLPP
Target Bioclass
Enzyme
Family
Class-I aminoacyl-tRNA synthetase family
Subcellular location
Mitochondrion matrix
Function Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of tRNA(Trp).
Uniprot ID
Q9UGM6
Ensemble ID
ENST00000235521.5
HGNC ID
HGNC:12730

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MOLT4 SNV: p.A277V .
SCC25 SNV: p.A144G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K143(6.67); K199(6.25); K226(1.09)  LDD0277  [1]
Probe 1
 Probe Info  		Y264(9.80)  LDD3495  [2]
DBIA
 Probe Info  		C102(2.94)  LDD3319  [3]
BTD
 Probe Info  		C350(1.25)  LDD2117  [4]
Acrolein
 Probe Info  		N.A.  LDD0224  [5]
ATP probe
 Probe Info  		K229(0.00); K253(0.00); K226(0.00)  LDD0199  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C278
 Probe Info  		81.57  LDD1948  [8]
VE-P
 Probe Info  		N.A.  LDD0396  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C350(1.25)  LDD2117  [4]
 LDCM0022  KB02 22RV1 C102(1.17)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C102(1.82)  LDD2660  [3]
 LDCM0024  KB05 MEWO C102(2.94)  LDD3319  [3]
 LDCM0109  NEM HeLa N.A.  LDD0224  [5]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Tryptophan Small molecular drug DB00150

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.