Details of the Target

General Information of Target

Target ID LDTP13067
Target Name Cingulin (CGN)
Gene Name CGN
Gene ID 57530
Synonyms
KIAA1319; Cingulin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTDGKLSTSTNGVAFMGILDGRPGNPLQNLQHVNLKAPRLLSAPEYGPKLKLRALEDRHS
LQSVDSGIPTLEIGNPEPVPCSAVHVRRKQSDSDLIPERAFQSACALPSCAPPAPSSTER
EQSVRKSSTFPRTGYDSVKLYSPTSKALTRSDDVSVCSVSSLGTELSTTLSVSNEDILDL
VVTSSSSAIVTLENDDDPQFTNVTLSSIKETRGLHQQDCVHEAEEGSKLKILGPFSNFFA
RNLLARKQSARLDKHNDLGWKLFGKAPLRENAQKDSKRIQKEYEDKAGRPSKPPSPKQNV
RKNLDFEPLSTTALILEDRPANLPAKPAEEAQKHRQQYEEMVVQAKKRELKEAQRRKKQL
EERCRVEESIGNAVLTWNNEILPNWETMWCSRKVRDLWWQGIPPSVRGKVWSLAIGNELN
ITHELFDICLARAKERWRSLSTGGSEVENEDAGFSAADREASLELIKLDISRTFPNLCIF
QQGGPYHDMLHSILGAYTCYRPDVGYVQGMSFIAAVLILNLDTADAFIAFSNLLNKPCQM
AFFRVDHGLMLTYFAAFEVFFEENLPKLFAHFKKNNLTPDIYLIDWIFTLYSKSLPLDLA
CRIWDVFCRDGEEFLFRTALGILKLFEDILTKMDFIHMAQFLTRLPEDLPAEELFASIAT
IQMQSRNKKWAQVLTALQKDSREMEKGSPSLRH
Target Bioclass
Other
Family
Cingulin family
Subcellular location
Cell junction, tight junction
Function Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Uniprot ID
Q9P2M7
Ensemble ID
ENST00000271636.12
HGNC ID
HGNC:17429

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 Substitution: p.S1022F .
HCC15 SNV: p.Q716H .
HCT15 SNV: p.L465S .
HEC1 SNV: p.Q645Ter .
HEC1B SNV: p.Q645Ter .
IGROV1 SNV: p.Q63Ter .
LS180 SNV: p.V18F DBIA    Probe Info 
LU65 SNV: p.M550T .
MCC13 SNV: p.D852N .
MEWO SNV: p.P220S .
RCC10RGB SNV: p.L819P .
TOV21G SNV: p.S238N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y105(11.97)  LDD3495  [1]
DBIA
 Probe Info  		C691(1.22)  LDD3312  [2]
IA-alkyne
 Probe Info  		C697(0.00); C1018(0.00); C403(0.00)  LDD0162  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C691(1.02)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C691(1.25)  LDD1509  [6]
 LDCM0277  AC18 HEK-293T C691(0.96)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C691(1.10)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C691(0.95)  LDD1518  [6]
 LDCM0284  AC24 HEK-293T C691(0.79)  LDD1523  [6]
 LDCM0286  AC26 HEK-293T C691(0.96)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C691(1.17)  LDD1526  [6]
 LDCM0290  AC3 HEK-293T C691(1.18)  LDD1529  [6]
 LDCM0293  AC32 HEK-293T C691(0.72)  LDD1532  [6]
 LDCM0295  AC34 HEK-293T C691(0.94)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C691(1.35)  LDD1535  [6]
 LDCM0302  AC40 HEK-293T C691(0.94)  LDD1541  [6]
 LDCM0304  AC42 HEK-293T C691(0.89)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C691(1.13)  LDD1544  [6]
 LDCM0310  AC48 HEK-293T C691(1.07)  LDD1549  [6]
 LDCM0313  AC50 HEK-293T C691(0.91)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C691(1.13)  LDD1553  [6]
 LDCM0319  AC56 HEK-293T C691(0.95)  LDD1558  [6]
 LDCM0321  AC58 HEK-293T C691(0.92)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C691(1.10)  LDD1561  [6]
 LDCM0328  AC64 HEK-293T C691(1.12)  LDD1567  [6]
 LDCM0345  AC8 HEK-293T C691(1.06)  LDD1569  [6]
 LDCM0275  AKOS034007705 HEK-293T C691(0.98)  LDD1514  [6]
 LDCM0372  CL103 HEK-293T C691(1.08)  LDD1576  [6]
 LDCM0376  CL107 HEK-293T C691(1.07)  LDD1580  [6]
 LDCM0381  CL111 HEK-293T C691(1.11)  LDD1585  [6]
 LDCM0385  CL115 HEK-293T C691(1.13)  LDD1589  [6]
 LDCM0389  CL119 HEK-293T C691(1.08)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C691(0.74)  LDD1594  [6]
 LDCM0394  CL123 HEK-293T C691(1.03)  LDD1598  [6]
 LDCM0398  CL127 HEK-293T C691(1.09)  LDD1602  [6]
 LDCM0402  CL15 HEK-293T C691(1.23)  LDD1606  [6]
 LDCM0405  CL18 HEK-293T C691(1.11)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C691(1.18)  LDD1610  [6]
 LDCM0412  CL24 HEK-293T C691(1.27)  LDD1616  [6]
 LDCM0415  CL27 HEK-293T C691(1.06)  LDD1619  [6]
 LDCM0418  CL3 HEK-293T C691(1.07)  LDD1622  [6]
 LDCM0419  CL30 HEK-293T C691(1.06)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C691(1.47)  LDD1624  [6]
 LDCM0425  CL36 HEK-293T C691(0.92)  LDD1629  [6]
 LDCM0428  CL39 HEK-293T C691(1.04)  LDD1632  [6]
 LDCM0432  CL42 HEK-293T C691(0.90)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C691(1.32)  LDD1637  [6]
 LDCM0438  CL48 HEK-293T C691(1.15)  LDD1642  [6]
 LDCM0445  CL54 HEK-293T C691(1.05)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C691(1.31)  LDD1649  [6]
 LDCM0451  CL6 HEK-293T C691(1.08)  LDD1654  [6]
 LDCM0452  CL60 HEK-293T C691(0.83)  LDD1655  [6]
 LDCM0455  CL63 HEK-293T C691(1.03)  LDD1658  [6]
 LDCM0458  CL66 HEK-293T C691(1.04)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C691(1.20)  LDD1662  [6]
 LDCM0462  CL7 HEK-293T C691(1.05)  LDD1665  [6]
 LDCM0465  CL72 HEK-293T C691(1.03)  LDD1668  [6]
 LDCM0471  CL78 HEK-293T C691(0.97)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C691(1.27)  LDD1675  [6]
 LDCM0478  CL84 HEK-293T C691(0.80)  LDD1681  [6]
 LDCM0481  CL87 HEK-293T C691(1.09)  LDD1684  [6]
 LDCM0485  CL90 HEK-293T C691(0.99)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C691(1.31)  LDD1689  [6]
 LDCM0491  CL96 HEK-293T C691(1.07)  LDD1694  [6]
 LDCM0494  CL99 HEK-293T C691(1.09)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C691(1.04)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C691(1.12)  LDD1671  [6]
 LDCM0022  KB02 HEK-293T C691(0.87)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C691(0.98)  LDD1497  [6]
 LDCM0024  KB05 HMCB C691(1.22)  LDD3312  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402