Details of the Target

General Information of Target

Target ID LDTP13011
Target Name 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (PLCE1)
Gene Name PLCE1
Gene ID 51196
Synonyms
KIAA1516; PLCE; PPLC; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; EC 3.1.4.11; Pancreas-enriched phospholipase C; Phosphoinositide phospholipase C-epsilon-1; Phospholipase C-epsilon-1; PLC-epsilon-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARAGPRLVLSEEAVRAKSGLGPHRDLAELQSLSIPGTYQEKITHLGHSLMSLTGLKSLD
LSRNSLVSLEGIQYLTALESLNLYYNCISSLAEVFRLHALTELVDVDFRLNPVVKVEPDY
RLFVVHLLPKLQQLDDRPVRASERKASRLHFASEDSLDSKESVPASLKEGRPHHPRAKCT
EALAKQSLVMDADDEAVLNLIAECEWDLGRPPGSTSFSQKGREADSRGSQESRHLLSPQL
VQYQCGDSGKQGRETRRSSCRGCCLEKMPWSQLCGELPPLYGAEPEASRAPRPHTYFTPH
PDSMDTEDSASSQKLDLSGEMVPGPLPAPGKCRKRRMPVGRFQTFSDQEGLGCPERTHGS
SVPKESLSRQDSSESRNGRTLSQPEASETEEQRSRGVTDTREPSPGSHSALPGKKTALQA
ALLETLLDLVDRSWGGCRSLHSNEAFLAQARHILSSVEEFTAAQDSSAMVGEDVGSLALE
SKSLQSRLAEQQQQHAREMSEVTAELHHTHKELDDLRQHLDKSLEENSRLKSLLLSMKKE
VKSADTAATLNLQIAGLQTSVKRLCGEIVELKQHLEHYDKIQELTQMLQESHSSLVSTNE
HLLQELSQVRAQHRAEVEQMHWSYQELKKTMALFPHSSASHGGCQAC
Target Bioclass
Enzyme
Subcellular location
Cytoplasm, cytosol
Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. In podocytes, is involved in the regulation of lamellipodia formation. Acts downstream of AVIL to allow ARP2/3 complex assembly.
Uniprot ID
Q9P212
Ensemble ID
ENST00000371375.2
HGNC ID
HGNC:17175

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8305C SNV: p.Y1401H .
A375 SNV: p.K2110E .
CAL51 SNV: p.T692I .
CCK81 SNV: p.E498Ter .
HEC1 SNV: p.Q587R; p.R729W .
KMCH1 SNV: p.S1477P; p.V2018M .
KYSE180 SNV: p.N1944S .
L428 SNV: p.E1368Q .
MEWO SNV: p.S9F; p.L559I; p.E1657K .
MOLT4 Deletion: p.T2111PfsTer6
SNV: p.G176D		 .
NCIH1155 SNV: p.V385M .
OVK18 SNV: p.R729W .
PC14 SNV: p.E1354Ter .
RCC10RGB SNV: p.R1907L .
REH SNV: p.Q2043Ter .
RL952 SNV: p.Q2140R .
SUIT2 SNV: p.R1093K .
SUPT1 SNV: p.L136F .
TF1 SNV: p.G2249S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C441(5.19)  LDD3373  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [2]
WYneN
 Probe Info  		N.A.  LDD0021  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0068  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C441(1.04)  LDD1507  [5]
 LDCM0259  AC14 HEK-293T C441(1.15)  LDD1512  [5]
 LDCM0276  AC17 HEK-293T C441(0.95)  LDD1515  [5]
 LDCM0281  AC21 HEK-293T C441(0.99)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C441(0.98)  LDD1521  [5]
 LDCM0285  AC25 HEK-293T C441(0.97)  LDD1524  [5]
 LDCM0289  AC29 HEK-293T C441(1.03)  LDD1528  [5]
 LDCM0291  AC30 HEK-293T C441(0.90)  LDD1530  [5]
 LDCM0294  AC33 HEK-293T C441(1.03)  LDD1533  [5]
 LDCM0298  AC37 HEK-293T C441(1.09)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C441(1.14)  LDD1538  [5]
 LDCM0303  AC41 HEK-293T C441(1.04)  LDD1542  [5]
 LDCM0307  AC45 HEK-293T C441(1.09)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C441(1.02)  LDD1547  [5]
 LDCM0311  AC49 HEK-293T C441(1.00)  LDD1550  [5]
 LDCM0312  AC5 HEK-293T C441(1.03)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C441(0.99)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C441(1.09)  LDD1556  [5]
 LDCM0320  AC57 HEK-293T C441(0.98)  LDD1559  [5]
 LDCM0323  AC6 HEK-293T C441(1.15)  LDD1562  [5]
 LDCM0325  AC61 HEK-293T C441(1.04)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C441(1.06)  LDD1565  [5]
 LDCM0248  AKOS034007472 HEK-293T C441(1.02)  LDD1511  [5]
 LDCM0356  AKOS034007680 HEK-293T C441(0.98)  LDD1570  [5]
 LDCM0367  CL1 HEK-293T C441(1.08)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C441(1.45)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C441(1.09)  LDD1574  [5]
 LDCM0374  CL105 HEK-293T C441(1.07)  LDD1578  [5]
 LDCM0378  CL109 HEK-293T C441(1.34)  LDD1582  [5]
 LDCM0383  CL113 HEK-293T C441(1.04)  LDD1587  [5]
 LDCM0387  CL117 HEK-293T C441(1.26)  LDD1591  [5]
 LDCM0392  CL121 HEK-293T C441(1.27)  LDD1596  [5]
 LDCM0396  CL125 HEK-293T C441(1.02)  LDD1600  [5]
 LDCM0400  CL13 HEK-293T C441(1.23)  LDD1604  [5]
 LDCM0404  CL17 HEK-293T C441(1.30)  LDD1608  [5]
 LDCM0409  CL21 HEK-293T C441(1.26)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C441(1.02)  LDD1614  [5]
 LDCM0413  CL25 HEK-293T C441(1.20)  LDD1617  [5]
 LDCM0417  CL29 HEK-293T C441(0.91)  LDD1621  [5]
 LDCM0422  CL33 HEK-293T C441(1.49)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C441(1.22)  LDD1627  [5]
 LDCM0426  CL37 HEK-293T C441(1.04)  LDD1630  [5]
 LDCM0431  CL41 HEK-293T C441(1.00)  LDD1635  [5]
 LDCM0435  CL45 HEK-293T C441(1.18)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C441(1.14)  LDD1640  [5]
 LDCM0439  CL49 HEK-293T C441(1.20)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C441(1.09)  LDD1644  [5]
 LDCM0444  CL53 HEK-293T C441(1.02)  LDD1647  [5]
 LDCM0448  CL57 HEK-293T C441(1.20)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C441(1.20)  LDD1652  [5]
 LDCM0453  CL61 HEK-293T C441(1.05)  LDD1656  [5]
 LDCM0457  CL65 HEK-293T C441(1.05)  LDD1660  [5]
 LDCM0461  CL69 HEK-293T C441(1.07)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C441(1.16)  LDD1666  [5]
 LDCM0466  CL73 HEK-293T C441(1.23)  LDD1669  [5]
 LDCM0470  CL77 HEK-293T C441(1.00)  LDD1673  [5]
 LDCM0475  CL81 HEK-293T C441(1.04)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C441(1.14)  LDD1679  [5]
 LDCM0479  CL85 HEK-293T C441(1.21)  LDD1682  [5]
 LDCM0483  CL89 HEK-293T C441(1.11)  LDD1686  [5]
 LDCM0484  CL9 HEK-293T C441(1.05)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C441(1.10)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C441(1.25)  LDD1692  [5]
 LDCM0492  CL97 HEK-293T C441(1.14)  LDD1695  [5]
 LDCM0022  KB02 BRX211 C441(2.88)  LDD2268  [1]
 LDCM0023  KB03 BRX211 C441(4.80)  LDD2685  [1]
 LDCM0024  KB05 OCUG-1 C441(5.19)  LDD3373  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
4 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402