Details of the Target

General Information of Target

Target ID LDTP12910
Target Name Diphosphoinositol polyphosphate phosphohydrolase 2 (NUDT4)
Gene Name NUDT4
Gene ID 11163
Synonyms
DIPP2; KIAA0487; Diphosphoinositol polyphosphate phosphohydrolase 2; DIPP-2; EC 3.6.1.52; Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2; Nucleoside diphosphate-linked moiety X motif 4; Nudix motif 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGASDPEVAPWARGGAAGMAGAGAGAGARGGAAAGVEARARDPPPAHRAHPRHPRPAAQP
SARRMDGGSGGLGSGDNAPTTEALFVALGAGVTALSHPLLYVKLLIQVGHEPMPPTLGTN
VLGRKVLYLPSFFTYAKYIVQVDGKIGLFRGLSPRLMSNALSTVTRGSMKKVFPPDEIEQ
VSNKDDMKTSLKKVVKETSYEMMMQCVSRMLAHPLHVISMRCMVQFVGREAKYSGVLSSI
GKIFKEEGLLGFFVGLIPHLLGDVVFLWGCNLLAHFINAYLVDDSVSDTPGGLGNDQNPG
SQFSQALAIRSYTKFVMGIAVSMLTYPFLLVGDLMAVNNCGLQAGLPPYSPVFKSWIHCW
KYLSVQGQLFRGSSLLFRRVSSGSCFALE
Target Bioclass
Enzyme
Family
Nudix hydrolase family, DIPP subfamily
Subcellular location
Cytoplasm
Function
Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Can also catalyze the hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) but not diadenosine 5',5'''-P1,P5-pentaphosphate (Ap5A) and the major reaction products are ADP and p4a from Ap6A. Also able to hydrolyze 5-phosphoribose 1-diphosphate. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
Uniprot ID
Q9NZJ9
Ensemble ID
ENST00000337179.9
HGNC ID
HGNC:8051
ChEMBL ID
CHEMBL4295967

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HUH7 SNV: p.S40I .
MDAMB453 SNV: p.E15K .
OVK18 SNV: p.A63V .
SW1116 SNV: p.D13E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		11.85  LDD0403  [1]
AHL-Pu-1
 Probe Info  		C131(4.91)  LDD0168  [2]
DBIA
 Probe Info  		C147(52.49)  LDD0209  [3]
HHS-475
 Probe Info  		Y68(0.77)  LDD0264  [4]
IA-alkyne
 Probe Info  		C131(0.00); C147(0.00)  LDD0162  [5]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [6]
NAIA_5
 Probe Info  		C148(0.00); C132(0.00)  LDD2224  [7]
IPM
 Probe Info  		C131(0.00); C147(0.00)  LDD0147  [6]
AOyne
 Probe Info  		15.00  LDD0443  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C131(4.91)  LDD0168  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C131(8.65)  LDD0169  [2]
 LDCM0156  Aniline NCI-H1299 11.85  LDD0403  [1]
 LDCM0625  F8 Ramos C131(4.89); C147(0.92)  LDD2187  [9]
 LDCM0572  Fragment10 Ramos C131(3.11); C147(1.01)  LDD2189  [9]
 LDCM0573  Fragment11 Ramos C131(3.32); C147(0.17)  LDD2190  [9]
 LDCM0574  Fragment12 Ramos C131(1.07); C147(0.68)  LDD2191  [9]
 LDCM0575  Fragment13 Ramos C131(0.54); C147(0.97)  LDD2192  [9]
 LDCM0576  Fragment14 Ramos C131(0.73); C147(0.64)  LDD2193  [9]
 LDCM0579  Fragment20 Ramos C131(0.97); C147(0.58)  LDD2194  [9]
 LDCM0580  Fragment21 Ramos C131(0.48); C147(0.90)  LDD2195  [9]
 LDCM0582  Fragment23 Ramos C131(1.30); C147(1.12)  LDD2196  [9]
 LDCM0578  Fragment27 Ramos C131(1.32); C147(0.90)  LDD2197  [9]
 LDCM0586  Fragment28 Ramos C131(0.73)  LDD2198  [9]
 LDCM0588  Fragment30 Ramos C131(0.99); C147(0.77)  LDD2199  [9]
 LDCM0589  Fragment31 Ramos C131(0.78); C147(0.97)  LDD2200  [9]
 LDCM0590  Fragment32 Ramos C131(2.46); C147(0.92)  LDD2201  [9]
 LDCM0468  Fragment33 Ramos C131(0.67); C147(1.00)  LDD2202  [9]
 LDCM0596  Fragment38 Ramos C131(1.25); C147(0.81)  LDD2203  [9]
 LDCM0566  Fragment4 Ramos C131(0.71); C147(0.80)  LDD2184  [9]
 LDCM0610  Fragment52 Ramos C147(0.56)  LDD2204  [9]
 LDCM0614  Fragment56 Ramos C131(0.57); C147(0.82)  LDD2205  [9]
 LDCM0569  Fragment7 Ramos C131(0.81); C147(0.58)  LDD2186  [9]
 LDCM0571  Fragment9 Ramos C147(0.79)  LDD2188  [9]
 LDCM0116  HHS-0101 DM93 Y68(0.77)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y68(0.82)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y68(0.74)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y68(1.07)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y68(0.90)  LDD0268  [4]
 LDCM0022  KB02 Ramos C131(1.01); C147(0.71)  LDD2182  [9]
 LDCM0023  KB03 Jurkat C147(52.49)  LDD0209  [3]
 LDCM0024  KB05 Ramos C131(0.76); C147(0.66)  LDD2185  [9]
 LDCM0627  NUDT7-COV-1 HEK-293T C131(0.91)  LDD2206  [10]
 LDCM0628  OTUB2-COV-1 HEK-293T C131(0.72)  LDD2207  [10]
 LDCM0131  RA190 MM1.R C147(1.24)  LDD0304  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kelch-like ECH-associated protein 1 (KEAP1) KEAP1 family Q14145
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
10 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
11 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.