Details of the Target

General Information of Target

Target ID LDTP12136
Target Name E3 ubiquitin-protein ligase pellino homolog 2 (PELI2)
Gene Name PELI2
Gene ID 57161
Synonyms
E3 ubiquitin-protein ligase pellino homolog 2; Pellino-2; EC 2.3.2.27; RING-type E3 ubiquitin transferase pellino homolog 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGFGTPGATVTVT
LRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQQTLEKINFTLRVHDVLFGDV
WLCSGQSNMQMTVLQIFNATRELSNTAAYQSVRILSVSPIQAEQELEDLVAVDLQWSKPT
SENLGHGYFKYMSAVCWLFGRHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQ
GSIPYDSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDW
RETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYVPNPKMPNTFM
AVAMDLCDRDSPFGSIHPRDKQTVAYRLHLGARALAYGEKNLTFEGPLPEKIELLAHKGL
LNLTYYQQIQVQKKDNKIFEISCCSDHRCKWLPASMNTVSTQSLTLAIDSCHGTVVALRY
AWTTWPCEYKQCPLYHPSSALPAPPFIAFITDQGPGHQSNVAK
Target Bioclass
Enzyme
Family
Pellino family
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.
Uniprot ID
Q9HAT8
Ensemble ID
ENST00000267460.9
HGNC ID
HGNC:8828

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K47(10.00)  LDD0277  [1]
DBIA
 Probe Info  		C284(32.45)  LDD0209  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C214(0.00); C145(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]
Compound 11
 Probe Info  		N.A.  LDD2213  [4]
IPM
 Probe Info  		N.A.  LDD2156  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C249(0.95)  LDD1510  [6]
 LDCM0280  AC20 HEK-293T C249(1.00)  LDD1519  [6]
 LDCM0288  AC28 HEK-293T C249(1.08)  LDD1527  [6]
 LDCM0297  AC36 HEK-293T C249(0.96)  LDD1536  [6]
 LDCM0301  AC4 HEK-293T C249(1.03)  LDD1540  [6]
 LDCM0306  AC44 HEK-293T C249(0.96)  LDD1545  [6]
 LDCM0315  AC52 HEK-293T C249(0.97)  LDD1554  [6]
 LDCM0324  AC60 HEK-293T C249(0.91)  LDD1563  [6]
 LDCM0367  CL1 HEK-293T C284(1.06)  LDD1571  [6]
 LDCM0369  CL100 HEK-293T C249(0.99)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C284(1.06)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C249(0.95)  LDD1575  [6]
 LDCM0373  CL104 HEK-293T C249(1.08)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C284(1.12)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C249(0.93)  LDD1579  [6]
 LDCM0377  CL108 HEK-293T C249(0.99)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C284(0.96)  LDD1582  [6]
 LDCM0380  CL110 HEK-293T C249(0.97)  LDD1584  [6]
 LDCM0382  CL112 HEK-293T C249(0.90)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C284(1.06)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C249(0.94)  LDD1588  [6]
 LDCM0386  CL116 HEK-293T C249(1.16)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C284(1.03)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C249(1.02)  LDD1592  [6]
 LDCM0391  CL120 HEK-293T C249(0.99)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C284(1.03)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C249(0.98)  LDD1597  [6]
 LDCM0395  CL124 HEK-293T C249(0.96)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C284(1.17)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C249(1.14)  LDD1601  [6]
 LDCM0399  CL128 HEK-293T C249(0.95)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C284(0.99)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C249(1.17)  LDD1605  [6]
 LDCM0403  CL16 HEK-293T C249(1.04)  LDD1607  [6]
 LDCM0407  CL2 HEK-293T C249(1.08)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C249(1.07)  LDD1612  [6]
 LDCM0413  CL25 HEK-293T C284(1.11)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C249(1.03)  LDD1618  [6]
 LDCM0416  CL28 HEK-293T C249(1.01)  LDD1620  [6]
 LDCM0421  CL32 HEK-293T C249(1.01)  LDD1625  [6]
 LDCM0426  CL37 HEK-293T C284(1.07)  LDD1630  [6]
 LDCM0429  CL4 HEK-293T C249(0.95)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C249(1.01)  LDD1634  [6]
 LDCM0434  CL44 HEK-293T C249(1.03)  LDD1638  [6]
 LDCM0439  CL49 HEK-293T C284(1.11)  LDD1643  [6]
 LDCM0441  CL50 HEK-293T C249(0.97)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C249(1.10)  LDD1646  [6]
 LDCM0447  CL56 HEK-293T C249(0.98)  LDD1650  [6]
 LDCM0453  CL61 HEK-293T C284(1.10)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C249(0.97)  LDD1657  [6]
 LDCM0456  CL64 HEK-293T C249(1.09)  LDD1659  [6]
 LDCM0460  CL68 HEK-293T C249(0.89)  LDD1663  [6]
 LDCM0466  CL73 HEK-293T C284(1.04)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C249(1.06)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C249(0.87)  LDD1672  [6]
 LDCM0473  CL8 HEK-293T C249(0.88)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C249(0.97)  LDD1677  [6]
 LDCM0479  CL85 HEK-293T C284(1.19)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C249(1.06)  LDD1683  [6]
 LDCM0482  CL88 HEK-293T C249(1.03)  LDD1685  [6]
 LDCM0487  CL92 HEK-293T C249(0.93)  LDD1690  [6]
 LDCM0492  CL97 HEK-293T C284(1.15)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C249(0.99)  LDD1696  [6]
 LDCM0427  Fragment51 HEK-293T C249(0.98)  LDD1631  [6]
 LDCM0023  KB03 Jurkat C284(32.45)  LDD0209  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hyaluronidase-2 (HYAL2) Glycosyl hydrolase 56 family Q12891
Interleukin-1 receptor-associated kinase 1 (IRAK1) TKL Ser/Thr protein kinase family P51617
Interleukin-1 receptor-associated kinase 4 (IRAK4) TKL Ser/Thr protein kinase family Q9NWZ3
Neuronal-specific septin-3 (SEPTIN3) Septin GTPase family Q9UH03
E3 ubiquitin-protein ligase TRIM32 (TRIM32) TRIM/RBCC family Q13049
Polycomb group RING finger protein 2 (PCGF2) . P35227
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MyoD family inhibitor (MDFI) MDFI family Q99750
Nuclear pore glycoprotein p62 (NUP62) Nucleoporin NSP1/NUP62 family P37198
Transmembrane protein 79 (TMEM79) . Q9BSE2
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leucine zipper transcription factor-like protein 1 (LZTFL1) LZTFL1 family Q9NQ48
Tumor protein 63 (TP63) P53 family Q9H3D4
Transcription factor Sp4 (SP4) Sp1 C2H2-type zinc-finger protein family Q02446
Homeobox protein MOX-1 (MEOX1) . P50221
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Olfactory receptor 7D2 (OR7D2) G-protein coupled receptor 1 family Q96RA2
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Rab GTPase-binding effector protein 1 (RABEP1) Rabaptin family Q15276
Regulator of microtubule dynamics protein 3 (RMDN3) RMDN family Q96TC7
C-type lectin domain family 17, member A (CLEC17A) . Q6ZS10

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402