Details of the Target

General Information of Target

Target ID LDTP12036
Target Name N-alpha-acetyltransferase 60 (NAA60)
Gene Name NAA60
Gene ID 79903
Synonyms
HAT4; NAT15; N-alpha-acetyltransferase 60; hNaa60; EC 2.3.1.259; Histone acetyltransferase type B protein 4; HAT4; EC 2.3.1.48; N-acetyltransferase 15; N-alpha-acetyltransferase F; NatF
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDGIIEQKSMLVHSKISDAGKRNGLINTRNLMAESRDGLVSVYPAPQYQSHRVGASTVPA
SLDSSRSEPMQQLLDPNTLQQSVESRYRPNIILYSEGVLRSWGDGVAADCCETTFIEDRS
PTKDSLEYPDGKFIDLSADDIKIHTLSYDVEEEEEFQELESDYSSDTESEDNFLMMPPRD
HLGLSVFSMLCCFWPLGIAAFYLSHETNKAVAKGDLHQASTSSRRALFLAVLSITIGTGV
YVGVAVALIAYLSKNNHL
Target Bioclass
Enzyme
Family
Acetyltransferase family, NAA60 subfamily
Subcellular location
Golgi apparatus membrane
Function
N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.
Uniprot ID
Q9H7X0
Ensemble ID
ENST00000360862.9
HGNC ID
HGNC:25875
ChEMBL ID
CHEMBL4630856

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.D173Y .
MELJUSO SNV: p.I194V DBIA    Probe Info 
RKO SNV: p.M66T .
SAOS2 SNV: p.V5A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C19(2.11)  LDD3312  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C132(0.59)  LDD1510  [2]
 LDCM0280  AC20 HEK-293T C132(0.56)  LDD1519  [2]
 LDCM0288  AC28 HEK-293T C132(0.77)  LDD1527  [2]
 LDCM0297  AC36 HEK-293T C132(0.51)  LDD1536  [2]
 LDCM0301  AC4 HEK-293T C132(0.66)  LDD1540  [2]
 LDCM0306  AC44 HEK-293T C132(0.50)  LDD1545  [2]
 LDCM0315  AC52 HEK-293T C132(0.81)  LDD1554  [2]
 LDCM0324  AC60 HEK-293T C132(0.52)  LDD1563  [2]
 LDCM0369  CL100 HEK-293T C30(1.22)  LDD1573  [2]
 LDCM0373  CL104 HEK-293T C30(0.93)  LDD1577  [2]
 LDCM0377  CL108 HEK-293T C30(1.07)  LDD1581  [2]
 LDCM0382  CL112 HEK-293T C30(0.80)  LDD1586  [2]
 LDCM0386  CL116 HEK-293T C30(0.94)  LDD1590  [2]
 LDCM0391  CL120 HEK-293T C30(1.31)  LDD1595  [2]
 LDCM0395  CL124 HEK-293T C30(1.12)  LDD1599  [2]
 LDCM0399  CL128 HEK-293T C30(0.89)  LDD1603  [2]
 LDCM0403  CL16 HEK-293T C30(1.22)  LDD1607  [2]
 LDCM0408  CL20 HEK-293T C132(0.68)  LDD1612  [2]
 LDCM0416  CL28 HEK-293T C30(0.94)  LDD1620  [2]
 LDCM0421  CL32 HEK-293T C132(0.01)  LDD1625  [2]
 LDCM0429  CL4 HEK-293T C30(1.09)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C30(1.10)  LDD1634  [2]
 LDCM0434  CL44 HEK-293T C132(0.63)  LDD1638  [2]
 LDCM0443  CL52 HEK-293T C30(1.03)  LDD1646  [2]
 LDCM0447  CL56 HEK-293T C132(0.00)  LDD1650  [2]
 LDCM0456  CL64 HEK-293T C30(0.83)  LDD1659  [2]
 LDCM0460  CL68 HEK-293T C132(0.39)  LDD1663  [2]
 LDCM0469  CL76 HEK-293T C30(1.16)  LDD1672  [2]
 LDCM0473  CL8 HEK-293T C132(0.56)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C132(0.62)  LDD1677  [2]
 LDCM0482  CL88 HEK-293T C30(1.06)  LDD1685  [2]
 LDCM0487  CL92 HEK-293T C132(0.52)  LDD1690  [2]
 LDCM0022  KB02 769-P C19(1.46)  LDD2246  [1]
 LDCM0023  KB03 769-P C19(2.39)  LDD2663  [1]
 LDCM0024  KB05 HMCB C19(2.11)  LDD3312  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402