Details of the Target

General Information of Target

Target ID LDTP11514
Target Name Dedicator of cytokinesis protein 9 (DOCK9)
Gene Name DOCK9
Gene ID 23348
Synonyms
KIAA1058; ZIZ1; Dedicator of cytokinesis protein 9; Cdc42 guanine nucleotide exchange factor zizimin-1; Zizimin-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRRLGPFHPRVHWAAPPSLSSGLHRLLFLRGTRIPSSTTLSPPRHDSLSLDGGTVNPPRV
REPTGREAFGPSPASSDWLPARWRNGRGGRPRARLCSGWTAAEEARRNPTLGGLLGRQRL
LLRMGGGRLGAPMERHGRASATSVSSAGEQAAGDPEGRRQEPLRRRASSASVPAVGASAE
GTRRDRLGSYSGPTSVSRQRVESLRKKRPLFPWFGLDIGGTLVKLVYFEPKDITAEEEEE
EVESLKSIRKYLTSNVAYGSTGIRDVHLELKDLTLCGRKGNLHFIRFPTHDMPAFIQMGR
DKNFSSLHTVFCATGGGAYKFEQDFLTIGDLQLCKLDELDCLIKGILYIDSVGFNGRSQC
YYFENPADSEKCQKLPFDLKNPYPLLLVNIGSGVSILAVYSKDNYKRVTGTSLGGGTFFG
LCCLLTGCTTFEEALEMASRGDSTKVDKLVRDIYGGDYERFGLPGWAVASSFGNMMSKEK
REAVSKEDLARATLITITNNIGSIARMCALNENINQVVFVGNFLRINTIAMRLLAYALDY
WSKGQLKALFSEHEGYFGAVGALLELLKIP
Target Bioclass
Other
Family
DOCK family
Subcellular location
Endomembrane system
Function Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.
Uniprot ID
Q9BZ29
Ensemble ID
ENST00000376460.5
HGNC ID
HGNC:14132

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K506(10.00); K61(5.14); K62(6.67)  LDD0277  [1]
DBIA
 Probe Info  		C667(2.04); C468(1.70); C628(2.06)  LDD3312  [2]
AHL-Pu-1
 Probe Info  		C628(2.33)  LDD0171  [3]
HHS-475
 Probe Info  		Y78(1.88)  LDD0264  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
NAIA_5
 Probe Info  		N.A.  LDD2224  [6]
IPM
 Probe Info  		C69(0.00); C613(0.00)  LDD0147  [7]
TFBX
 Probe Info  		C69(0.00); C1553(0.00)  LDD0148  [7]
AOyne
 Probe Info  		11.50  LDD0443  [8]
HHS-465
 Probe Info  		N.A.  LDD2240  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C628(2.33)  LDD0171  [3]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C1682(0.34)  LDD1702  [10]
 LDCM0625  F8 Ramos C1292(1.13)  LDD2187  [11]
 LDCM0572  Fragment10 Ramos C1292(0.96)  LDD2189  [11]
 LDCM0574  Fragment12 Ramos C1292(0.56)  LDD2191  [11]
 LDCM0575  Fragment13 Ramos C1292(0.67)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C1292(0.84)  LDD2193  [11]
 LDCM0579  Fragment20 Ramos C1292(0.46)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C1292(2.11)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C1292(1.52)  LDD2196  [11]
 LDCM0586  Fragment28 Ramos C1292(0.21)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C1292(0.77)  LDD2199  [11]
 LDCM0596  Fragment38 Ramos C1292(0.81)  LDD2203  [11]
 LDCM0566  Fragment4 Ramos C1292(0.78)  LDD2184  [11]
 LDCM0610  Fragment52 Ramos C1292(0.74)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C1292(0.92)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C1292(0.99)  LDD2186  [11]
 LDCM0571  Fragment9 Ramos C1292(0.85)  LDD2188  [11]
 LDCM0116  HHS-0101 DM93 Y78(1.88)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y78(2.39)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y78(1.53)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y78(1.89)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y78(1.24)  LDD0268  [4]
 LDCM0022  KB02 HEK-293T C490(1.06); C94(0.95); C628(0.98)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C490(1.02); C94(1.10); C628(1.06)  LDD1497  [12]
 LDCM0024  KB05 HMCB C667(2.04); C468(1.70); C628(2.06)  LDD3312  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 2 (SMAD2) Dwarfin/SMAD family Q15796
Mothers against decapentaplegic homolog 3 (SMAD3) Dwarfin/SMAD family P84022

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
10 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402