Details of the Target

General Information of Target

Target ID LDTP11278
Target Name Protein adenylyltransferase FICD (FICD)
Gene Name FICD
Gene ID 11153
Synonyms
HIP13; HYPE; Protein adenylyltransferase FICD; EC 2.7.7.108; AMPylator FICD; De-AMPylase FICD; EC 3.1.4.-; FIC domain-containing protein; Huntingtin yeast partner E; Huntingtin-interacting protein 13; HIP-13; Huntingtin-interacting protein E
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA
Target Bioclass
Enzyme
Family
Fic family
Subcellular location
Endoplasmic reticulum membrane
Function
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
Uniprot ID
Q9BVA6
Ensemble ID
ENST00000552695.6
HGNC ID
HGNC:18416

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C75(1.19)  LDD1507  [1]
NAIA_4
 Probe Info  		N.A.  LDD2226  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C75(1.19)  LDD1507  [1]
 LDCM0237  AC12 HEK-293T C75(0.98)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C75(1.20)  LDD1512  [1]
 LDCM0276  AC17 HEK-293T C75(0.96)  LDD1515  [1]
 LDCM0280  AC20 HEK-293T C75(1.01)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C75(0.73)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C75(0.76)  LDD1521  [1]
 LDCM0285  AC25 HEK-293T C75(1.05)  LDD1524  [1]
 LDCM0288  AC28 HEK-293T C75(1.25)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C75(0.77)  LDD1528  [1]
 LDCM0291  AC30 HEK-293T C75(1.19)  LDD1530  [1]
 LDCM0294  AC33 HEK-293T C75(1.10)  LDD1533  [1]
 LDCM0297  AC36 HEK-293T C75(1.06)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C75(0.83)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C75(0.80)  LDD1538  [1]
 LDCM0301  AC4 HEK-293T C75(1.14)  LDD1540  [1]
 LDCM0303  AC41 HEK-293T C75(1.07)  LDD1542  [1]
 LDCM0306  AC44 HEK-293T C75(1.08)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C75(1.04)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C75(0.98)  LDD1547  [1]
 LDCM0311  AC49 HEK-293T C75(1.34)  LDD1550  [1]
 LDCM0312  AC5 HEK-293T C75(0.76)  LDD1551  [1]
 LDCM0315  AC52 HEK-293T C75(1.03)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C75(0.90)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C75(1.47)  LDD1556  [1]
 LDCM0320  AC57 HEK-293T C75(1.42)  LDD1559  [1]
 LDCM0323  AC6 HEK-293T C75(1.27)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C75(0.97)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C75(0.87)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C75(1.03)  LDD1565  [1]
 LDCM0248  AKOS034007472 HEK-293T C75(0.86)  LDD1511  [1]
 LDCM0356  AKOS034007680 HEK-293T C75(1.52)  LDD1570  [1]
 LDCM0367  CL1 HEK-293T C75(1.38)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C75(1.42)  LDD1572  [1]
 LDCM0370  CL101 HEK-293T C75(1.04)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C75(1.26)  LDD1575  [1]
 LDCM0374  CL105 HEK-293T C75(1.05)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C75(0.94)  LDD1579  [1]
 LDCM0378  CL109 HEK-293T C75(1.02)  LDD1582  [1]
 LDCM0380  CL110 HEK-293T C75(1.01)  LDD1584  [1]
 LDCM0383  CL113 HEK-293T C75(1.22)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C75(1.47)  LDD1588  [1]
 LDCM0387  CL117 HEK-293T C75(1.30)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C75(1.34)  LDD1592  [1]
 LDCM0392  CL121 HEK-293T C75(1.43)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C75(1.00)  LDD1597  [1]
 LDCM0396  CL125 HEK-293T C75(0.88)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C75(0.80)  LDD1601  [1]
 LDCM0400  CL13 HEK-293T C75(1.33)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C75(0.97)  LDD1605  [1]
 LDCM0404  CL17 HEK-293T C75(1.08)  LDD1608  [1]
 LDCM0407  CL2 HEK-293T C75(0.98)  LDD1611  [1]
 LDCM0408  CL20 HEK-293T C75(1.62)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C75(0.72)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C75(1.40)  LDD1614  [1]
 LDCM0413  CL25 HEK-293T C75(1.37)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C75(1.01)  LDD1618  [1]
 LDCM0417  CL29 HEK-293T C75(1.21)  LDD1621  [1]
 LDCM0421  CL32 HEK-293T C75(1.17)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C75(0.55)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C75(1.61)  LDD1627  [1]
 LDCM0426  CL37 HEK-293T C75(1.20)  LDD1630  [1]
 LDCM0431  CL41 HEK-293T C75(1.27)  LDD1635  [1]
 LDCM0434  CL44 HEK-293T C75(1.59)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C75(0.68)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C75(1.42)  LDD1640  [1]
 LDCM0439  CL49 HEK-293T C75(1.20)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C75(1.13)  LDD1644  [1]
 LDCM0441  CL50 HEK-293T C75(1.31)  LDD1645  [1]
 LDCM0444  CL53 HEK-293T C75(1.41)  LDD1647  [1]
 LDCM0447  CL56 HEK-293T C75(1.04)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C75(0.83)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C75(1.08)  LDD1652  [1]
 LDCM0453  CL61 HEK-293T C75(1.73)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C75(1.22)  LDD1657  [1]
 LDCM0457  CL65 HEK-293T C75(1.11)  LDD1660  [1]
 LDCM0460  CL68 HEK-293T C75(1.33)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C75(0.75)  LDD1664  [1]
 LDCM0463  CL70 HEK-293T C75(1.27)  LDD1666  [1]
 LDCM0466  CL73 HEK-293T C75(1.07)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C75(0.86)  LDD1670  [1]
 LDCM0470  CL77 HEK-293T C75(1.78)  LDD1673  [1]
 LDCM0473  CL8 HEK-293T C75(0.88)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C75(0.85)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C75(0.75)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C75(1.25)  LDD1679  [1]
 LDCM0479  CL85 HEK-293T C75(1.04)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C75(0.99)  LDD1683  [1]
 LDCM0483  CL89 HEK-293T C75(1.17)  LDD1686  [1]
 LDCM0484  CL9 HEK-293T C75(0.86)  LDD1687  [1]
 LDCM0487  CL92 HEK-293T C75(1.31)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C75(0.62)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C75(1.14)  LDD1692  [1]
 LDCM0492  CL97 HEK-293T C75(1.32)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C75(1.08)  LDD1696  [1]
 LDCM0427  Fragment51 HEK-293T C75(1.22)  LDD1631  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein adenylyltransferase FICD (FICD) Fic family Q9BVA6
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264