Details of the Target

General Information of Target

Target ID LDTP10300
Target Name E3 ubiquitin-protein ligase pellino homolog 1 (PELI1)
Gene Name PELI1
Gene ID 57162
Synonyms
PRISM; E3 ubiquitin-protein ligase pellino homolog 1; Pellino-1; EC 2.3.2.27; Pellino-related intracellular-signaling molecule; RING-type E3 ubiquitin transferase pellino homolog 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATDWLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDIT
ELKILEIPGPGDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPKR
TDVKSQDVAVSPQQQQCSKSYVDRHMESLSQSKSFRRRHNSWSSSSRHPNQATPKKSGLK
NGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPNER
PTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLT
LERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANH
DVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLR
DQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIG
IPQQVFQEVGINYHSPFGCKFVIPLHSA
Target Bioclass
Enzyme
Family
Pellino family
Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
Uniprot ID
Q96FA3
Ensemble ID
ENST00000358912.5
HGNC ID
HGNC:8827

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C61(1.12)  LDD2182  [1]
DBIA
 Probe Info  		C282(0.86)  LDD1508  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C282(0.86)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C282(1.00)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C282(1.09)  LDD1510  [2]
 LDCM0277  AC18 HEK-293T C282(0.77)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C282(1.03)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C282(0.81)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C282(1.03)  LDD1519  [2]
 LDCM0286  AC26 HEK-293T C282(0.92)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C282(0.86)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C282(1.22)  LDD1527  [2]
 LDCM0290  AC3 HEK-293T C282(0.95)  LDD1529  [2]
 LDCM0295  AC34 HEK-293T C282(0.92)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C282(1.05)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C282(0.86)  LDD1536  [2]
 LDCM0301  AC4 HEK-293T C282(1.16)  LDD1540  [2]
 LDCM0304  AC42 HEK-293T C282(1.04)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C282(1.16)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C282(1.08)  LDD1545  [2]
 LDCM0313  AC50 HEK-293T C282(1.17)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C282(0.91)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C282(0.95)  LDD1554  [2]
 LDCM0321  AC58 HEK-293T C282(1.10)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C282(0.99)  LDD1561  [2]
 LDCM0324  AC60 HEK-293T C282(1.33)  LDD1563  [2]
 LDCM0405  CL18 HEK-293T C282(1.05)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C282(1.37)  LDD1610  [2]
 LDCM0408  CL20 HEK-293T C282(0.71)  LDD1612  [2]
 LDCM0419  CL30 HEK-293T C282(0.92)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C282(1.33)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C282(0.62)  LDD1625  [2]
 LDCM0432  CL42 HEK-293T C282(0.95)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C282(1.05)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C282(0.68)  LDD1638  [2]
 LDCM0445  CL54 HEK-293T C282(0.71)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C282(1.17)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C282(0.78)  LDD1650  [2]
 LDCM0451  CL6 HEK-293T C282(0.68)  LDD1654  [2]
 LDCM0458  CL66 HEK-293T C282(0.95)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C282(1.01)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C282(0.80)  LDD1663  [2]
 LDCM0462  CL7 HEK-293T C282(1.20)  LDD1665  [2]
 LDCM0471  CL78 HEK-293T C282(0.96)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C282(1.00)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C282(1.31)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C282(0.79)  LDD1677  [2]
 LDCM0485  CL90 HEK-293T C282(1.16)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C282(1.01)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C282(0.92)  LDD1690  [2]
 LDCM0625  F8 Ramos C61(1.57)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C61(0.89)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C61(6.76)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C61(0.60)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C61(0.80)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C61(0.84)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C61(0.45)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C61(0.85)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C61(0.84)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C61(1.01)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C61(0.65)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C61(0.90)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C61(0.72)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C61(1.11)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C61(0.89)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C61(0.99)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C61(0.97)  LDD2184  [1]
 LDCM0610  Fragment52 Ramos C61(0.93)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C61(0.99)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C61(0.80)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C61(0.62)  LDD2188  [1]
 LDCM0022  KB02 Ramos C61(1.12)  LDD2182  [1]
 LDCM0023  KB03 Ramos C61(0.97)  LDD2183  [1]
 LDCM0024  KB05 Ramos C61(0.86)  LDD2185  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pachytene checkpoint protein 2 homolog (TRIP13) AAA ATPase family Q15645
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A (INPP5J) Inositol 1,4,5-trisphosphate 5-phosphatase type II family Q15735
Interleukin-1 receptor-associated kinase 1 (IRAK1) TKL Ser/Thr protein kinase family P51617
Interleukin-1 receptor-associated kinase 4 (IRAK4) TKL Ser/Thr protein kinase family Q9NWZ3
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
WASH complex subunit 3 (WASHC3) CCDC53 family Q9Y3C0
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tumor protein 63 (TP63) P53 family Q9H3D4
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Neutrophil gelatinase-associated lipocalin (LCN2) Lipocalin family P80188
Golgi SNAP receptor complex member 2 (GOSR2) GOSR2 family O14653
Prelamin-A/C (LMNA) Intermediate filament family P02545
28 kDa heat- and acid-stable phosphoprotein (PDAP1) PDAP1 family Q13442
Protein VAC14 homolog (VAC14) VAC14 family Q08AM6
C-type lectin domain family 17, member A (CLEC17A) . Q6ZS10
Centrobin (CNTROB) . Q8N137
Regulating synaptic membrane exocytosis protein 4 (RIMS4) . Q9H426

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019