General Information of Target

Target ID LDTP10212
Target Name U8 snoRNA-decapping enzyme (NUDT16)
Gene Name NUDT16
Gene ID 131870
Synonyms
U8 snoRNA-decapping enzyme; EC 3.6.1.62; IDP phosphatase; IDPase; EC 3.6.1.64; Inosine diphosphate phosphatase; Nucleoside diphosphate-linked moiety X motif 16; Nudix motif 16; Nudix hydrolase 16; U8 snoRNA-binding protein H29K; m7GpppN-mRNA hydrolase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSY
LESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLK
VFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQI
TALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
Target Bioclass
Enzyme
Family
Nudix hydrolase family, NUDT16 subfamily
Subcellular location
Nucleus
Function
RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
Uniprot ID
Q96DE0
Ensemble ID
ENST00000502852.1
HGNC ID
HGNC:26442

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.G101V .
LS180 SNV: p.H194Q .
MCC13 Substitution: p.S162F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HHS-475
 Probe Info 
Y43(1.12)  LDD0264  [1]
DBIA
 Probe Info 
C23(0.94)  LDD1507  [2]
ATP probe
 Probe Info 
N.A.  LDD0199  [3]
NAIA_5
 Probe Info 
N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C23(0.94)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C23(1.04)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C23(1.05)  LDD1509  [2]
 LDCM0259  AC14 HEK-293T C23(1.03)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C23(0.92)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C23(0.98)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C23(0.91)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C23(0.95)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C23(1.02)  LDD1518  [2]
 LDCM0282  AC22 HEK-293T C23(0.73)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C23(0.80)  LDD1522  [2]
 LDCM0285  AC25 HEK-293T C23(0.95)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C23(0.97)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C23(1.09)  LDD1526  [2]
 LDCM0290  AC3 HEK-293T C23(0.99)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C23(1.23)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C23(0.90)  LDD1531  [2]
 LDCM0294  AC33 HEK-293T C23(1.01)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C23(1.05)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C23(0.99)  LDD1535  [2]
 LDCM0299  AC38 HEK-293T C23(1.03)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C23(0.85)  LDD1539  [2]
 LDCM0303  AC41 HEK-293T C23(1.03)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C23(0.98)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C23(1.07)  LDD1544  [2]
 LDCM0308  AC46 HEK-293T C23(1.07)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C23(0.91)  LDD1548  [2]
 LDCM0311  AC49 HEK-293T C23(1.02)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C23(1.01)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C23(1.09)  LDD1553  [2]
 LDCM0317  AC54 HEK-293T C23(1.26)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C23(1.12)  LDD1557  [2]
 LDCM0320  AC57 HEK-293T C23(0.98)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C23(1.21)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C23(1.15)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C23(1.17)  LDD1562  [2]
 LDCM0326  AC62 HEK-293T C23(1.28)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C23(0.97)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C23(1.16)  LDD1568  [2]
 LDCM0356  AKOS034007680 HEK-293T C23(1.14)  LDD1570  [2]
 LDCM0368  CL10 HEK-293T C23(0.76)  LDD1572  [2]
 LDCM0372  CL103 HEK-293T C23(0.95)  LDD1576  [2]
 LDCM0376  CL107 HEK-293T C23(0.93)  LDD1580  [2]
 LDCM0379  CL11 HEK-293T C23(0.95)  LDD1583  [2]
 LDCM0381  CL111 HEK-293T C23(0.99)  LDD1585  [2]
 LDCM0385  CL115 HEK-293T C23(0.90)  LDD1589  [2]
 LDCM0389  CL119 HEK-293T C23(1.14)  LDD1593  [2]
 LDCM0394  CL123 HEK-293T C23(0.95)  LDD1598  [2]
 LDCM0398  CL127 HEK-293T C23(0.91)  LDD1602  [2]
 LDCM0402  CL15 HEK-293T C23(1.04)  LDD1606  [2]
 LDCM0404  CL17 HEK-293T C23(0.92)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C23(1.02)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C23(1.05)  LDD1610  [2]
 LDCM0410  CL22 HEK-293T C23(0.82)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C23(0.91)  LDD1615  [2]
 LDCM0415  CL27 HEK-293T C23(0.97)  LDD1619  [2]
 LDCM0417  CL29 HEK-293T C23(0.96)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C23(0.90)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C23(1.18)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C23(1.07)  LDD1624  [2]
 LDCM0423  CL34 HEK-293T C23(0.76)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C23(1.06)  LDD1628  [2]
 LDCM0428  CL39 HEK-293T C23(0.98)  LDD1632  [2]
 LDCM0431  CL41 HEK-293T C23(1.11)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C23(1.00)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C23(1.05)  LDD1637  [2]
 LDCM0436  CL46 HEK-293T C23(0.72)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C23(1.25)  LDD1641  [2]
 LDCM0440  CL5 HEK-293T C23(1.09)  LDD1644  [2]
 LDCM0444  CL53 HEK-293T C23(1.14)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C23(1.15)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C23(1.09)  LDD1649  [2]
 LDCM0449  CL58 HEK-293T C23(0.79)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C23(1.02)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C23(0.83)  LDD1654  [2]
 LDCM0455  CL63 HEK-293T C23(1.12)  LDD1658  [2]
 LDCM0457  CL65 HEK-293T C23(1.06)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C23(0.94)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C23(1.09)  LDD1662  [2]
 LDCM0462  CL7 HEK-293T C23(1.00)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C23(0.88)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C23(1.11)  LDD1667  [2]
 LDCM0470  CL77 HEK-293T C23(0.98)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C23(1.02)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C23(1.12)  LDD1675  [2]
 LDCM0476  CL82 HEK-293T C23(0.83)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C23(0.89)  LDD1680  [2]
 LDCM0481  CL87 HEK-293T C23(1.03)  LDD1684  [2]
 LDCM0483  CL89 HEK-293T C23(1.01)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C23(1.02)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C23(1.15)  LDD1689  [2]
 LDCM0489  CL94 HEK-293T C23(1.00)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C23(0.62)  LDD1693  [2]
 LDCM0494  CL99 HEK-293T C23(0.99)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C23(0.99)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C23(1.05)  LDD1671  [2]
 LDCM0116  HHS-0101 DM93 Y43(1.12)  LDD0264  [1]
 LDCM0117  HHS-0201 DM93 Y43(0.27)  LDD0265  [1]
 LDCM0118  HHS-0301 DM93 Y43(0.53)  LDD0266  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
U8 snoRNA-decapping enzyme (NUDT16) Nudix hydrolase family Q96DE0

References

1 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264