Details of the Target

General Information of Target

Target ID LDTP10146
Target Name Dedicator of cytokinesis protein 10 (DOCK10)
Gene Name DOCK10
Gene ID 55619
Synonyms
KIAA0694; ZIZ3; Dedicator of cytokinesis protein 10; Zizimin-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTLRLLEDWCRGMDMNPRKALLIAGISQSCSVAEIEEALQAGLAPLGEYRLLGRMFRRDE
NRKVALVGLTAETSHALVPKEIPGKGGIWRVIFKPPDPDNTFLSRLNEFLAGEGMTVGEL
SRALGHENGSLDPEQGMIPEMWAPMLAQALEALQPALQCLKYKKLRVFSGRESPEPGEEE
FGRWMFHTTQMIKAWQVPDVEKRRRLLESLRGPALDVIRVLKINNPLITVDECLQALEEV
FGVTDNPRELQVKYLTTYQKDEEKLSAYVLRLEPLLQKLVQRGAIERDAVNQARLDQVIA
GAVHKTIRRELNLPEDGPAPGFLQLLVLIKDYEAAEEEEALLQAILEGNFT
Target Bioclass
Other
Family
DOCK family
Subcellular location
Nucleus
Function
Guanine nucleotide-exchange factor (GEF) that activates CDC42 and RAC1 by exchanging bound GDP for free GTP. Essential for dendritic spine morphogenesis in Purkinje cells and in hippocampal neurons, via a CDC42-mediated pathway. Sustains B-cell lymphopoiesis in secondary lymphoid tissues and regulates FCER2/CD23 expression.
Uniprot ID
Q96BY6
Ensemble ID
ENST00000258390.12
HGNC ID
HGNC:23479

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.R1116G .
AN3CA SNV: p.Y93C; p.K692R .
CAL148 SNV: p.E2035D .
DU145 SNV: p.Q1249R .
ETK1 SNV: p.V1229I .
HCC827 SNV: p.V845L DBIA    Probe Info 
HCT116 SNV: p.N691S .
HCT15 SNV: p.E893K; p.E2051G .
HEC1 SNV: p.L455F .
HEC1B SNV: p.L455F .
HT SNV: p.D1068G .
IGR1 SNV: p.E1958G .
IGROV1 SNV: p.I134S .
JHH7 SNV: p.A2071D .
KO52 SNV: p.S2169R DBIA    Probe Info 
LNCaP clone FGC SNV: p.R46M .
LUDLU1 SNV: p.W1740Ter .
MCC13 SNV: p.P808S .
MEWO SNV: p.R1879C DBIA    Probe Info 
MINO SNV: p.D1137Y .
NCIH1155 SNV: p.A1711T DBIA    Probe Info 
NCIH1993 SNV: p.Q1457H .
NCIH2172 SNV: p.L92S .
OZ SNV: p.G3S .
PC9 SNV: p.C238F .
RKO SNV: p.V1076I .
RL952 SNV: p.G309C .
SKOV3 SNV: p.P364Q .
SNU1 Insertion: p.V425CfsTer7
SNV: p.R1660H		 DBIA    Probe Info 
SSP25 SNV: p.V1229I .
SUDHL6 SNV: p.L1916F .
SW756 SNV: p.S151F .
TOV21G SNV: p.K1959N .
ZR751 SNV: p.E1679K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.91  LDD0402  [1]
DBIA
 Probe Info  		C2164(0.99); C1508(0.96); C1510(0.96)  LDD0078  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C1063(0.00); C2164(0.00); C1722(0.00); C2108(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		C1375(0.00); C936(0.00); C1722(0.00); C1063(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		C1063(0.00); C936(0.00); C2108(0.00); C867(0.00)  LDD0037  [3]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0276  AC17 PaTu 8988t C2164(1.30)  LDD1155  [2]
 LDCM0277  AC18 PaTu 8988t C2164(1.96)  LDD1156  [2]
 LDCM0278  AC19 PaTu 8988t C2164(0.85)  LDD1157  [2]
 LDCM0280  AC20 PaTu 8988t C2164(0.96)  LDD1159  [2]
 LDCM0281  AC21 PaTu 8988t C2164(1.35)  LDD1160  [2]
 LDCM0282  AC22 PaTu 8988t C2164(1.11)  LDD1161  [2]
 LDCM0283  AC23 PaTu 8988t C2164(1.41)  LDD1162  [2]
 LDCM0284  AC24 PaTu 8988t C2164(1.24)  LDD1163  [2]
 LDCM0156  Aniline NCI-H1299 9.47  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C2164(0.99); C1508(0.96); C1510(0.96)  LDD0078  [2]
 LDCM0374  CL105 PaTu 8988t C2164(1.07)  LDD1253  [2]
 LDCM0375  CL106 PaTu 8988t C2164(1.28)  LDD1254  [2]
 LDCM0376  CL107 PaTu 8988t C2164(0.89)  LDD1255  [2]
 LDCM0377  CL108 PaTu 8988t C2164(1.43)  LDD1256  [2]
 LDCM0378  CL109 PaTu 8988t C2164(1.81)  LDD1257  [2]
 LDCM0380  CL110 PaTu 8988t C2164(1.70)  LDD1259  [2]
 LDCM0381  CL111 PaTu 8988t C2164(1.46)  LDD1260  [2]
 LDCM0436  CL46 PaTu 8988t C2164(0.89)  LDD1315  [2]
 LDCM0437  CL47 PaTu 8988t C2164(0.99)  LDD1316  [2]
 LDCM0438  CL48 PaTu 8988t C2164(0.95)  LDD1317  [2]
 LDCM0439  CL49 PaTu 8988t C2164(0.93)  LDD1318  [2]
 LDCM0441  CL50 PaTu 8988t C2164(1.27)  LDD1320  [2]
 LDCM0442  CL51 PaTu 8988t C2164(1.16)  LDD1321  [2]
 LDCM0443  CL52 PaTu 8988t C2164(1.29)  LDD1322  [2]
 LDCM0444  CL53 PaTu 8988t C2164(2.56)  LDD1323  [2]
 LDCM0445  CL54 PaTu 8988t C2164(1.28)  LDD1324  [2]
 LDCM0446  CL55 PaTu 8988t C2164(1.04)  LDD1325  [2]
 LDCM0447  CL56 PaTu 8988t C2164(0.50)  LDD1326  [2]
 LDCM0448  CL57 PaTu 8988t C2164(2.23)  LDD1327  [2]
 LDCM0449  CL58 PaTu 8988t C2164(1.40)  LDD1328  [2]
 LDCM0450  CL59 PaTu 8988t C2164(1.68)  LDD1329  [2]
 LDCM0452  CL60 PaTu 8988t C2164(1.62)  LDD1331  [2]
 LDCM0453  CL61 PaTu 8988t C2164(0.91)  LDD1332  [2]
 LDCM0454  CL62 PaTu 8988t C2164(3.17)  LDD1333  [2]
 LDCM0455  CL63 PaTu 8988t C2164(1.67)  LDD1334  [2]
 LDCM0456  CL64 PaTu 8988t C2164(1.67)  LDD1335  [2]
 LDCM0457  CL65 PaTu 8988t C2164(1.29)  LDD1336  [2]
 LDCM0458  CL66 PaTu 8988t C2164(2.17)  LDD1337  [2]
 LDCM0459  CL67 PaTu 8988t C2164(1.41)  LDD1338  [2]
 LDCM0460  CL68 PaTu 8988t C2164(1.75)  LDD1339  [2]
 LDCM0461  CL69 PaTu 8988t C2164(2.55)  LDD1340  [2]
 LDCM0463  CL70 PaTu 8988t C2164(1.67)  LDD1342  [2]
 LDCM0464  CL71 PaTu 8988t C2164(1.36)  LDD1343  [2]
 LDCM0465  CL72 PaTu 8988t C2164(1.91)  LDD1344  [2]
 LDCM0466  CL73 PaTu 8988t C2164(2.16)  LDD1345  [2]
 LDCM0467  CL74 PaTu 8988t C2164(1.78)  LDD1346  [2]
 LDCM0469  CL76 PaTu 8988t C2164(0.76)  LDD1348  [2]
 LDCM0470  CL77 PaTu 8988t C2164(0.72)  LDD1349  [2]
 LDCM0471  CL78 PaTu 8988t C2164(0.88)  LDD1350  [2]
 LDCM0472  CL79 PaTu 8988t C2164(0.70)  LDD1351  [2]
 LDCM0474  CL80 PaTu 8988t C2164(0.64)  LDD1353  [2]
 LDCM0475  CL81 PaTu 8988t C2164(0.81)  LDD1354  [2]
 LDCM0476  CL82 PaTu 8988t C2164(1.25)  LDD1355  [2]
 LDCM0477  CL83 PaTu 8988t C2164(1.26)  LDD1356  [2]
 LDCM0478  CL84 PaTu 8988t C2164(0.70)  LDD1357  [2]
 LDCM0479  CL85 PaTu 8988t C2164(0.74)  LDD1358  [2]
 LDCM0480  CL86 PaTu 8988t C2164(0.92)  LDD1359  [2]
 LDCM0481  CL87 PaTu 8988t C2164(0.68)  LDD1360  [2]
 LDCM0482  CL88 PaTu 8988t C2164(2.65)  LDD1361  [2]
 LDCM0483  CL89 PaTu 8988t C2164(0.73)  LDD1362  [2]
 LDCM0485  CL90 PaTu 8988t C2164(1.74)  LDD1364  [2]
 LDCM0468  Fragment33 PaTu 8988t C2164(2.62)  LDD1347  [2]
 LDCM0022  KB02 697 C1092(1.96)  LDD2245  [6]
 LDCM0023  KB03 Jurkat C2164(80.41); C1063(11.58)  LDD0209  [7]
 LDCM0024  KB05 COLO792 C2164(3.42); C699(1.66)  LDD3310  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endophilin-B1 (SH3GLB1) Endophilin family Q9Y371
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Coiled-coil domain-containing protein 102B (CCDC102B) . Q68D86
Melanoma-associated antigen 11 (MAGEA11) . P43364

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
7 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.