Details of the Target

General Information of Target

Target ID LDTP09566
Target Name TBC1 domain family member 22A (TBC1D22A)
Gene Name TBC1D22A
Gene ID 25771
Synonyms
C22orf4; TBC1 domain family member 22A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASDGARKQFWKRSNSKLPGSIQHVYGAQHPPFDPLLHGTLLRSTAKMPTTPVKAKRVST
FQEFESNTSDAWDAGEDDDELLAMAAESLNSEVVMETANRVLRNHSQRQGRPTLQEGPGL
QQKPRPEAEPPSPPSGDLRLVKSVSESHTSCPAESASDAAPLQRSQSLPHSATVTLGGTS
DPSTLSSSALSEREASRLDKFKQLLAGPNTDLEELRRLSWSGIPKPVRPMTWKLLSGYLP
ANVDRRPATLQRKQKEYFAFIEHYYDSRNDEVHQDTYRQIHIDIPRMSPEALILQPKVTE
IFERILFIWAIRHPASGYVQGINDLVTPFFVVFICEYIEAEEVDTVDVSGVPAEVLCNIE
ADTYWCMSKLLDGIQDNYTFAQPGIQMKVKMLEELVSRIDEQVHRHLDQHEVRYLQFAFR
WMNNLLMREVPLRCTIRLWDTYQSEPDGFSHFHLYVCAAFLVRWRKEILEEKDFQELLLF
LQNLPTAHWDDEDISLLLAEAYRLKFAFADAPNHYKK
Target Bioclass
Other
Function May act as a GTPase-activating protein for Rab family protein(s).
Uniprot ID
Q8WUA7
Ensemble ID
ENST00000337137.9
HGNC ID
HGNC:1309

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
GAMG SNV: p.T149A .
HCT15 SNV: p.A459D .
Ishikawa (Heraklio) 02 ER Substitution: p.Y364_W365delinsTer .
KMCH1 SNV: p.E360D .
MELJUSO SNV: p.L204R .
NCIH1299 SNV: p.A83S m-APA    Probe Info 
NCIH1703 SNV: p.P52T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K202(5.00)  LDD0277  [1]
DBIA
 Probe Info  		C151(1.84)  LDD3378  [2]
AHL-Pu-1
 Probe Info  		C151(2.24)  LDD0169  [3]
Acrolein
 Probe Info  		N.A.  LDD0222  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
Compound 11
 Probe Info  		N.A.  LDD2213  [7]
IPM
 Probe Info  		N.A.  LDD0005  [8]
VSF
 Probe Info  		N.A.  LDD0007  [8]
Methacrolein
 Probe Info  		C151(0.00); H148(0.00)  LDD0218  [4]
m-APA
 Probe Info  		N.A.  LDD0404  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C151(2.24)  LDD0169  [3]
 LDCM0156  Aniline NCI-H1299 N.A.  LDD0404  [9]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0625  F8 Ramos C151(1.27)  LDD2187  [10]
 LDCM0572  Fragment10 Ramos C151(1.63)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C151(14.25)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C151(1.00)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C151(0.78)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C151(0.47)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C151(0.59)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C151(0.79)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C151(1.43)  LDD2196  [10]
 LDCM0578  Fragment27 Ramos C151(0.86)  LDD2197  [10]
 LDCM0586  Fragment28 Ramos C151(0.83)  LDD2198  [10]
 LDCM0588  Fragment30 Ramos C151(1.67)  LDD2199  [10]
 LDCM0589  Fragment31 Ramos C151(1.13)  LDD2200  [10]
 LDCM0590  Fragment32 Ramos C151(1.86)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C151(1.24)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C151(0.91)  LDD2203  [10]
 LDCM0566  Fragment4 Ramos C151(1.13)  LDD2184  [10]
 LDCM0610  Fragment52 Ramos C151(1.31)  LDD2204  [10]
 LDCM0614  Fragment56 Ramos C151(1.20)  LDD2205  [10]
 LDCM0569  Fragment7 Ramos C151(1.18)  LDD2186  [10]
 LDCM0571  Fragment9 Ramos C151(1.01)  LDD2188  [10]
 LDCM0022  KB02 Ramos C151(1.86)  LDD2182  [10]
 LDCM0023  KB03 Ramos C151(1.00)  LDD2183  [10]
 LDCM0024  KB05 OS-RC-2 C151(1.84)  LDD3378  [2]
 LDCM0131  RA190 MM1.R C151(1.09)  LDD0304  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisomal ATPase PEX1 (PEX1) AAA ATPase family O43933
NADH dehydrogenase flavoprotein 1, mitochondrial (NDUFV1) Complex I 51 kDa subunit family P49821
Transthyretin (TTR) Transthyretin family P02766
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgi resident protein GCP60 (ACBD3) . Q9H3P7
Wolframin (WFS1) . O76024

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
11 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.