Details of the Target

General Information of Target

Target ID LDTP09493
Target Name E3 ubiquitin-protein ligase SH3RF2 (SH3RF2)
Gene Name SH3RF2
Gene ID 153769
Synonyms
POSH3; POSHER; PPP1R39; RNF158; E3 ubiquitin-protein ligase SH3RF2; EC 2.3.2.27; Heart protein phosphatase 1-binding protein; HEPP1; POSH-eliminating RING protein; Protein phosphatase 1 regulatory subunit 39; RING finger protein 158; RING-type E3 ubiquitin transferase SH3RF2; SH3 domain-containing RING finger protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRVFKAHKELRCPECRTPVFSNI
EALPANLLLVRLLDGVRSGQSSGRGGSFRRPGTMTLQDGRKSRTNPRRLQASPFRLVPNV
RIHMDGVPRAKALCNYRGQNPGDLRFNKGDIILLRRQLDENWYQGEINGISGNFPASSVE
VIKQLPQPPPLCRALYNFDLRGKDKSENQDCLTFLKDDIITVISRVDENWAEGKLGDKVG
IFPILFVEPNLTARHLLEKNKGRQSSRTKNLSLVSSSSRGNTSTLRRGPGSRRKVPGQFS
ITTALNTLNRMVHSPSGRHMVEISTPVLISSSNPSVITQPMEKADVPSSCVGQVSTYHPA
PVSPGHSTAVVSLPGSQQHLSANMFVALHSYSAHGPDELDLQKGEGVRVLGKCQDGWLRG
VSLVTGRVGIFPNNYVIPIFRKTSSFPDSRSPGLYTTWTLSTSSVSSQGSISEGDPRQSR
PFKSVFVPTAIVNPVRSTAGPGTLGQGSLRKGRSSMRKNGSLQRPLQSGIPTLVVGSLRR
SPTMVLRPQQFQFYQPQGIPSSPSAVVVEMGSKPALTGEPALTCISRGSEAWIHSAASSL
IMEDKEIPIKSEPLPKPPASAPPSILVKPENSRNGIEKQVKTVRFQNYSPPPTKHYTSHP
TSGKPEQPATLKASQPEAASLGPEMTVLFAHRSGCHSGQQTDLRRKSALGKATTLVSTAS
GTQTVFPSK
Target Bioclass
Enzyme
Family
SH3RF family
Subcellular location
Nucleus
Function
Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity.
Uniprot ID
Q8TEC5
Ensemble ID
ENST00000359120.9
HGNC ID
HGNC:26299

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO679 SNV: p.P50L .
CORL23 SNV: p.P660S .
HCT15 SNV: p.V534A .
HT SNV: p.N260T .
Ishikawa (Heraklio) 02 ER SNV: p.L19P .
LOXIMVI SNV: p.S348Y .
LS180 SNV: p.T543N .
MCC13 SNV: p.P651S .
MEWO SNV: p.Q527Ter .
MOLT4 SNV: p.G85R .
NCIH2286 SNV: p.P91T; p.R524I .
OAW28 SNV: p.S620P .
OVK18 SNV: p.R419M .
SKMEL3 SNV: p.G529E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C15(2.02)  LDD1700  [1]
CY4
 Probe Info  		N.A.  LDD0247  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0520  AKOS000195272 MDA-MB-231 C15(1.10)  LDD2113  [1]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C15(1.04)  LDD2093  [1]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C15(0.98)  LDD2101  [1]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C15(0.87)  LDD2111  [1]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C15(0.42)  LDD2115  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C15(0.89)  LDD2125  [1]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C15(1.14)  LDD2129  [1]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C15(0.95)  LDD2133  [1]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C15(0.64)  LDD2134  [1]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C15(1.01)  LDD2135  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C15(0.94)  LDD2136  [1]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C15(0.63)  LDD2137  [1]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C15(2.02)  LDD1700  [1]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C15(0.52)  LDD2148  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) PPP phosphatase family P62136
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
A-kinase anchor protein 8-like (AKAP8L) AKAP95 family Q9ULX6
Angiomotin-like protein 2 (AMOTL2) Angiomotin family Q9Y2J4
Protein BANP (BANP) BANP/SMAR1 family Q8N9N5
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Vacuolar protein sorting-associated protein 37C (VPS37C) VPS37 family A5D8V6
BAG family molecular chaperone regulator 4 (BAG4) . O95429
Coiled-coil domain-containing protein 102B (CCDC102B) . Q68D86

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.