Details of the Target

General Information of Target

Target ID LDTP09467
Target Name Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 (ASAP3)
Gene Name ASAP3
Gene ID 55616
Synonyms
DDEFL1; UPLC1; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3; Development and differentiation-enhancing factor-like 1; Protein up-regulated in liver cancer 1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVR
AIHSSGLGHVENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNL
NNIVSFPLDSLMKGQLRDGRQDSKKQLEKAWKDYEAKMAKLEKERDRARVTGGIPGEVAQ
DMQRERRIFQLHMCEYLLKAGESQMKQGPDFLQSLIKFFHAQHNFFQDGWKAAQSLFPFI
EKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEHLSRKNSGCGYSIHQHQGNKQ
FGTEKVGFLYKKSDGIRRVWQKRKCGVKYGCLTISHSTINRPPVKLTLLTCQVRPNPEEK
KCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGE
PHDLTKLLIAEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQS
LTLDLLGPSELLLALNMGNTSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRF
ARRCTPEPQRLWTAICNRDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQA
SLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALD
IARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAH
WASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVS
SLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPS
EMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQL
LQD
Target Type
Literature-reported
Target Bioclass
Other
Subcellular location
Cytoplasm
Function Promotes cell proliferation.
TTD ID
T56207
Uniprot ID
Q8TDY4
DrugMap ID
TTB4ZEN
Ensemble ID
ENST00000336689.8
HGNC ID
HGNC:14987

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.A407T .
CHL1 SNV: p.S411T .
HEC1B SNV: p.G515D .
HUH7 SNV: p.A26S .
JURKAT SNV: p.R527H .
KMS27 SNV: p.L900H .
KYM1 SNV: p.C464Ter .
MCC13 SNV: p.P488S DBIA    Probe Info 
MEWO SNV: p.E359K .
MOLT4 SNV: p.R187H .
OZ SNV: p.E521D .
RCC10RGB SNV: p.R435S .
SKMEL3 SNV: p.A8S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K311(0.66)  LDD0277  [1]
DBIA
 Probe Info  		C556(0.91)  LDD0531  [2]
W1
 Probe Info  		N.A.  LDD0236  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C556(0.91)  LDD0531  [2]
 LDCM0226  AC11 HEK-293T C768(1.01)  LDD1509  [5]
 LDCM0276  AC17 HEK-293T C768(0.87)  LDD1515  [5]
 LDCM0278  AC19 HEK-293T C768(1.29)  LDD1517  [5]
 LDCM0279  AC2 HCT 116 C556(0.94)  LDD0596  [2]
 LDCM0285  AC25 HCT 116 C556(0.87)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C556(1.03)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C556(1.08)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C556(0.91)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C556(0.89)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C556(0.94)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C556(0.91)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C556(1.02)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C556(1.10)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C556(1.15)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C556(0.99)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C556(0.97)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C556(1.21)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C556(1.19)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C556(1.10)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C556(1.08)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C556(1.02)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C556(1.45)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C556(1.06)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C556(1.14)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C556(1.12)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C556(1.14)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C556(1.36)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C556(1.00)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C556(0.86)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C556(0.95)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C556(1.14)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C556(0.94)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C556(1.12)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C556(0.94)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C556(1.05)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C556(0.93)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C556(1.08)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C556(1.04)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C556(1.37)  LDD0636  [2]
 LDCM0320  AC57 HEK-293T C768(1.04)  LDD1559  [5]
 LDCM0322  AC59 HEK-293T C768(1.02)  LDD1561  [5]
 LDCM0356  AKOS034007680 HEK-293T C768(1.02)  LDD1570  [5]
 LDCM0632  CL-Sc Hep-G2 C331(0.79)  LDD2227  [4]
 LDCM0367  CL1 HEK-293T C768(0.99)  LDD1571  [5]
 LDCM0369  CL100 HCT 116 C556(0.85)  LDD0686  [2]
 LDCM0370  CL101 HEK-293T C768(1.05)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C768(1.13)  LDD1575  [5]
 LDCM0374  CL105 HEK-293T C768(0.98)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C768(1.04)  LDD1579  [5]
 LDCM0378  CL109 HEK-293T C768(0.91)  LDD1582  [5]
 LDCM0380  CL110 HEK-293T C768(1.08)  LDD1584  [5]
 LDCM0382  CL112 HCT 116 C556(0.89)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C556(0.98)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C556(0.97)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C556(1.02)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C556(0.87)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C556(1.41)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C556(1.08)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C556(1.33)  LDD0706  [2]
 LDCM0391  CL120 HCT 116 C556(1.02)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C556(0.87)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C556(1.02)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C556(1.06)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C556(1.14)  LDD0712  [2]
 LDCM0396  CL125 HEK-293T C768(0.97)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C768(1.12)  LDD1601  [5]
 LDCM0400  CL13 HEK-293T C768(1.00)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C768(1.12)  LDD1605  [5]
 LDCM0404  CL17 HEK-293T C768(0.82)  LDD1608  [5]
 LDCM0406  CL19 HEK-293T C768(0.99)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C768(1.11)  LDD1611  [5]
 LDCM0413  CL25 HEK-293T C768(0.78)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C768(1.04)  LDD1618  [5]
 LDCM0417  CL29 HEK-293T C768(0.79)  LDD1621  [5]
 LDCM0420  CL31 HEK-293T C768(1.15)  LDD1624  [5]
 LDCM0426  CL37 HEK-293T C768(0.91)  LDD1630  [5]
 LDCM0431  CL41 HEK-293T C768(0.92)  LDD1635  [5]
 LDCM0433  CL43 HEK-293T C768(1.09)  LDD1637  [5]
 LDCM0439  CL49 HEK-293T C768(1.04)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C768(0.90)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C768(1.00)  LDD1645  [5]
 LDCM0444  CL53 HEK-293T C768(0.81)  LDD1647  [5]
 LDCM0446  CL55 HEK-293T C768(1.09)  LDD1649  [5]
 LDCM0453  CL61 HEK-293T C768(1.05)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C768(1.02)  LDD1657  [5]
 LDCM0457  CL65 HEK-293T C768(0.99)  LDD1660  [5]
 LDCM0459  CL67 HEK-293T C768(1.00)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C768(0.98)  LDD1665  [5]
 LDCM0466  CL73 HEK-293T C768(1.10)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C768(1.07)  LDD1670  [5]
 LDCM0470  CL77 HEK-293T C768(0.97)  LDD1673  [5]
 LDCM0472  CL79 HEK-293T C768(0.96)  LDD1675  [5]
 LDCM0479  CL85 HEK-293T C768(1.08)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C768(1.18)  LDD1683  [5]
 LDCM0483  CL89 HEK-293T C768(1.02)  LDD1686  [5]
 LDCM0486  CL91 HCT 116 C556(0.82)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C556(0.83)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C556(0.93)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C556(0.87)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C556(0.79)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C556(0.81)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C556(0.78)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C556(0.81)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C556(1.07)  LDD0811  [2]
 LDCM0427  Fragment51 HEK-293T C768(1.08)  LDD1631  [5]
 LDCM0022  KB02 AN3-CA C60(2.34)  LDD2264  [6]
 LDCM0023  KB03 AN3-CA C60(2.89)  LDD2681  [6]
 LDCM0024  KB05 OAW-28 C768(1.36)  LDD3368  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AP-4 complex accessory subunit Tepsin (TEPSIN) . Q96N21
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-globin transcription factor CP2 (TFCP2) Grh/CP2 family Q12800
Proto-oncogene c-Rel (REL) . Q04864
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Adapter molecule crk (CRK) CRK family P46108
Na(+)/H(+) exchange regulatory cofactor NHE-RF4 (NHERF4) . Q86UT5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840