Details of the Target

General Information of Target

Target ID LDTP09291
Target Name Josephin-2 (JOSD2)
Gene Name JOSD2
Gene ID 126119
Synonyms
Josephin-2; EC 3.4.19.12; Josephin domain-containing protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSQAPGAQPSPPTVYHERQRLELCAVHALNNVLQQQLFSQEAADEICKRLAPDSRLNPHR
SLLGTGNYDVNVIMAALQGLGLAAVWWDRRRPLSQLALPQVLGLILNLPSPVSLGLLSLP
LRRRHWVALRQVDGVYYNLDSKLRAPEALGDEDGVRAFLAAALAQGLCEVLLVVTKEVEE
KGSWLRTD
Target Bioclass
Enzyme
Subcellular location
Cytoplasm, cytosol
Function Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.
Uniprot ID
Q8TAC2
Ensemble ID
ENST00000595669.5
HGNC ID
HGNC:28853
ChEMBL ID
CHEMBL4630845

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.Y15H .
HT115 SNV: p.A75T .
KYSE510 SNV: p.V101L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y137(14.26)  LDD3495  [1]
DBIA
 Probe Info  		C24(0.98); C47(0.98)  LDD1142  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0149  [3]
NAIA_5
 Probe Info  		C47(0.00); C24(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0263  AC143 PaTu 8988t C24(0.98); C47(0.98)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C24(1.00); C47(1.00)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C24(0.90); C47(0.90)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C24(0.90); C47(0.90)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C24(0.96); C47(0.96)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C24(0.94); C47(0.94)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C24(1.06); C47(1.06)  LDD1148  [2]
 LDCM0271  AC150 PaTu 8988t C24(1.02); C47(1.02)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C24(1.28); C47(1.28)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C24(1.36); C47(1.36)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C24(1.35); C47(1.35)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C24(1.31); C47(1.31)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C24(1.21); C47(1.21)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C24(1.09); C47(1.09)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C24(1.20); C47(1.20)  LDD2169  [2]
 LDCM0320  AC57 PaTu 8988t C24(1.04); C47(1.04)  LDD1199  [2]
 LDCM0321  AC58 PaTu 8988t C24(0.92); C47(0.92)  LDD1200  [2]
 LDCM0322  AC59 PaTu 8988t C24(1.23); C47(1.23)  LDD1201  [2]
 LDCM0324  AC60 PaTu 8988t C24(0.66); C47(0.66)  LDD1203  [2]
 LDCM0325  AC61 PaTu 8988t C24(0.87); C47(0.87)  LDD1204  [2]
 LDCM0326  AC62 PaTu 8988t C24(0.80); C47(0.80)  LDD1205  [2]
 LDCM0327  AC63 PaTu 8988t C24(0.76); C47(0.76)  LDD1206  [2]
 LDCM0328  AC64 PaTu 8988t C24(0.90); C47(0.90)  LDD1207  [2]
 LDCM0329  AC65 PaTu 8988t C24(1.04); C47(1.04)  LDD1208  [2]
 LDCM0330  AC66 PaTu 8988t C24(0.75); C47(0.75)  LDD1209  [2]
 LDCM0331  AC67 PaTu 8988t C24(0.69); C47(0.69)  LDD1210  [2]
 LDCM0332  AC68 PaTu 8988t C24(0.81); C47(0.81)  LDD1211  [2]
 LDCM0333  AC69 PaTu 8988t C24(0.81); C47(0.81)  LDD1212  [2]
 LDCM0335  AC70 PaTu 8988t C24(0.78); C47(0.78)  LDD1214  [2]
 LDCM0336  AC71 PaTu 8988t C24(0.94); C47(0.94)  LDD1215  [2]
 LDCM0337  AC72 PaTu 8988t C24(0.95); C47(0.95)  LDD1216  [2]
 LDCM0338  AC73 PaTu 8988t C24(0.81); C47(0.81)  LDD1217  [2]
 LDCM0339  AC74 PaTu 8988t C24(1.27); C47(1.27)  LDD1218  [2]
 LDCM0340  AC75 PaTu 8988t C24(1.07); C47(1.07)  LDD1219  [2]
 LDCM0341  AC76 PaTu 8988t C24(0.83); C47(0.83)  LDD1220  [2]
 LDCM0342  AC77 PaTu 8988t C24(0.78); C47(0.78)  LDD1221  [2]
 LDCM0343  AC78 PaTu 8988t C24(1.15); C47(1.15)  LDD1222  [2]
 LDCM0344  AC79 PaTu 8988t C24(0.64); C47(0.64)  LDD1223  [2]
 LDCM0346  AC80 PaTu 8988t C24(0.76); C47(0.76)  LDD1225  [2]
 LDCM0347  AC81 PaTu 8988t C24(0.93); C47(0.93)  LDD1226  [2]
 LDCM0348  AC82 PaTu 8988t C24(1.23); C47(1.23)  LDD1227  [2]
 LDCM0396  CL125 PaTu 8988t C24(0.90); C47(0.90)  LDD1275  [2]
 LDCM0397  CL126 PaTu 8988t C24(1.49); C47(1.49)  LDD1276  [2]
 LDCM0398  CL127 PaTu 8988t C24(0.92); C47(0.92)  LDD1277  [2]
 LDCM0399  CL128 PaTu 8988t C24(1.05); C47(1.05)  LDD1278  [2]
 LDCM0403  CL16 PaTu 8988t C24(1.35); C47(1.35)  LDD1282  [2]
 LDCM0404  CL17 PaTu 8988t C24(1.47); C47(1.47)  LDD1283  [2]
 LDCM0405  CL18 PaTu 8988t C24(1.41); C47(1.41)  LDD1284  [2]
 LDCM0406  CL19 PaTu 8988t C24(1.61); C47(1.61)  LDD1285  [2]
 LDCM0408  CL20 PaTu 8988t C24(2.72); C47(2.72)  LDD1287  [2]
 LDCM0409  CL21 PaTu 8988t C24(1.05); C47(1.05)  LDD1288  [2]
 LDCM0410  CL22 PaTu 8988t C24(0.94); C47(0.94)  LDD1289  [2]
 LDCM0411  CL23 PaTu 8988t C24(1.60); C47(1.60)  LDD1290  [2]
 LDCM0412  CL24 PaTu 8988t C24(1.05); C47(1.05)  LDD1291  [2]
 LDCM0413  CL25 PaTu 8988t C24(0.85); C47(0.85)  LDD1292  [2]
 LDCM0414  CL26 PaTu 8988t C24(1.03); C47(1.03)  LDD1293  [2]
 LDCM0415  CL27 PaTu 8988t C24(1.22); C47(1.22)  LDD1294  [2]
 LDCM0416  CL28 PaTu 8988t C24(1.31); C47(1.31)  LDD1295  [2]
 LDCM0417  CL29 PaTu 8988t C24(1.88); C47(1.88)  LDD1296  [2]
 LDCM0419  CL30 PaTu 8988t C24(1.23); C47(1.23)  LDD1298  [2]
 LDCM0453  CL61 PaTu 8988t C24(1.15); C47(1.15)  LDD1332  [2]
 LDCM0454  CL62 PaTu 8988t C24(1.23); C47(1.23)  LDD1333  [2]
 LDCM0455  CL63 PaTu 8988t C24(1.37); C47(1.37)  LDD1334  [2]
 LDCM0456  CL64 PaTu 8988t C24(1.90); C47(1.90)  LDD1335  [2]
 LDCM0457  CL65 PaTu 8988t C24(1.44); C47(1.44)  LDD1336  [2]
 LDCM0458  CL66 PaTu 8988t C24(0.98); C47(0.98)  LDD1337  [2]
 LDCM0459  CL67 PaTu 8988t C24(1.13); C47(1.13)  LDD1338  [2]
 LDCM0460  CL68 PaTu 8988t C24(1.55); C47(1.55)  LDD1339  [2]
 LDCM0461  CL69 PaTu 8988t C24(1.54); C47(1.54)  LDD1340  [2]
 LDCM0463  CL70 PaTu 8988t C24(1.06); C47(1.06)  LDD1342  [2]
 LDCM0464  CL71 PaTu 8988t C24(1.11); C47(1.11)  LDD1343  [2]
 LDCM0465  CL72 PaTu 8988t C24(1.27); C47(1.27)  LDD1344  [2]
 LDCM0466  CL73 PaTu 8988t C24(1.12); C47(1.12)  LDD1345  [2]
 LDCM0467  CL74 PaTu 8988t C24(1.14); C47(1.14)  LDD1346  [2]
 LDCM0468  Fragment33 PaTu 8988t C24(1.35); C47(1.35)  LDD1347  [2]
 LDCM0022  KB02 BRX142 C168(0.81)  LDD2267  [5]
 LDCM0023  KB03 BRX142 C168(2.34)  LDD2684  [5]
 LDCM0024  KB05 BRX142 C168(1.44)  LDD3101  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) Adenosylhomocysteinase family O43865
Atypical kinase COQ8A, mitochondrial (COQ8A) ADCK protein kinase family Q8NI60
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) . Q9Y680
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Synaptophysin (SYP) Synaptophysin/synaptobrevin family P08247
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Midkine (MDK) Pleiotrophin family P21741
Protein YIF1A (YIF1A) YIF1 family O95070
Alpha-tocopherol transfer protein (TTPA) . P49638

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840