Details of the Target

General Information of Target

Target ID LDTP09010
Target Name Protein SCAI (SCAI)
Gene Name SCAI
Gene ID 286205
Synonyms
C9orf126; Protein SCAI; Suppressor of cancer cell invasion protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVRGARQPQQPRSRLAPRLTGTVEKPPRKRRSRTEFALKEIMSSGGAEDDIPQGERKTVT
DFCYLLDKSKQLFNGLRDLPQYGQKQWQSYFGRTFDVYTKLWKFQQQHRQVLDNRYGLKR
WQIGEIASKIGQLYYHYYLRTSETSYLNEAFSFYSAIRQRSYYSQVNKEDRPELVVKKLR
YYARFIVVCLLLNKMDVVKDLVKELSDEIEDYTHRFNTEDQVEWNLVLQEVAAFIEADPV
MVLNDDNTIVITSNRLAETGAPLLEQGMIVGQLSLADALIIGNCNNQVKFSELTVDMFRM
LQALEREPMNLASQMNKPGMQESADKPTRRENPHKYLLYKPTFSQLYTFLAASFKELPAN
SVLLIYLSATGVFPTGRSDSEGPYDFGGVLTNSNRDIINGDAIHKRNQSHKEMHCLHPGD
LYPFTRKPLFIIVDSSNSVAYKNFTNLFGQPLVCLLSPTAYPKALQDQSQRGSLFTLFLN
NPLMAFLFVSGLSSMRRGLWEKCQEYLRKINRDIAQLLTHSRSIDQAFLQFFGDEFLRLL
LTRFIFCSATMRMHKIFRETRNYPESYPQLPRDETVENPHLQKHILELASILDVRNVFFE
NTIDDY
Target Bioclass
Other
Family
SCAI family
Subcellular location
Membrane
Function
Tumor suppressor which functions to suppress MRTFA-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
Uniprot ID
Q8N9R8
Ensemble ID
ENST00000336505.11
HGNC ID
HGNC:26709

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
GB1 SNV: p.T328I .
HEC1 SNV: p.D211E DBIA    Probe Info 
IPC298 SNV: p.S152P DBIA    Probe Info 
LNCaP clone FGC SNV: p.L456I DBIA    Probe Info 
SNGM SNV: p.R471Ter DBIA    Probe Info 
SW756 SNV: p.E565K DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C477(1.70)  LDD3332  [1]
BTD
 Probe Info  		C189(0.66)  LDD2150  [2]
Acrolein
 Probe Info  		N.A.  LDD0222  [3]
ATP probe
 Probe Info  		K68(0.00); K70(0.00)  LDD0199  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C63(0.00); C454(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C454(0.00); C284(0.00); C63(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C454(0.00); C63(0.00)  LDD0037  [5]
IPM
 Probe Info  		C189(0.00); C63(0.00)  LDD2156  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C63(0.99)  LDD1507  [9]
 LDCM0215  AC10 HEK-293T C63(0.98)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C63(0.97); C454(0.95)  LDD1509  [9]
 LDCM0259  AC14 HEK-293T C63(1.01)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C63(1.02)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C63(0.95)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C63(0.93)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C63(0.89); C454(0.92)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C63(1.02)  LDD1518  [9]
 LDCM0281  AC21 HEK-293T C63(1.02)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C63(1.01)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C63(1.00)  LDD1522  [9]
 LDCM0285  AC25 HEK-293T C63(0.98)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C63(1.00)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C63(1.03); C454(0.93)  LDD1526  [9]
 LDCM0289  AC29 HEK-293T C63(0.95)  LDD1528  [9]
 LDCM0290  AC3 HEK-293T C63(1.00); C454(1.05)  LDD1529  [9]
 LDCM0291  AC30 HEK-293T C63(0.96)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C63(1.14)  LDD1531  [9]
 LDCM0294  AC33 HEK-293T C63(1.03)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C63(1.01)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C63(1.10); C454(1.11)  LDD1535  [9]
 LDCM0298  AC37 HEK-293T C63(0.96)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C63(0.99)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C63(0.98)  LDD1539  [9]
 LDCM0303  AC41 HEK-293T C63(1.02)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C63(0.99)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C63(0.98); C454(1.02)  LDD1544  [9]
 LDCM0307  AC45 HEK-293T C63(1.00)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C63(1.03)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C63(1.09)  LDD1548  [9]
 LDCM0311  AC49 HEK-293T C63(1.00)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C63(1.09)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C63(0.96)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C63(0.97); C454(1.07)  LDD1553  [9]
 LDCM0316  AC53 HEK-293T C63(1.04)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C63(1.02)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C63(0.91)  LDD1557  [9]
 LDCM0320  AC57 HEK-293T C63(1.05)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C63(1.00)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C63(1.02); C454(1.08)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C63(1.05)  LDD1562  [9]
 LDCM0325  AC61 HEK-293T C63(0.98)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C63(0.98)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C63(1.00)  LDD1566  [9]
 LDCM0334  AC7 HEK-293T C63(1.00)  LDD1568  [9]
 LDCM0248  AKOS034007472 HEK-293T C63(1.05)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C63(1.04)  LDD1570  [9]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [3]
 LDCM0367  CL1 HEK-293T C454(1.01)  LDD1571  [9]
 LDCM0368  CL10 HEK-293T C63(1.37)  LDD1572  [9]
 LDCM0370  CL101 HEK-293T C454(1.03)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C63(1.18)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C454(0.92)  LDD1576  [9]
 LDCM0374  CL105 HEK-293T C454(1.08)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C63(1.09)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C454(1.03)  LDD1580  [9]
 LDCM0378  CL109 HEK-293T C454(1.05)  LDD1582  [9]
 LDCM0379  CL11 HEK-293T C63(0.99)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C63(1.17)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C454(1.00)  LDD1585  [9]
 LDCM0383  CL113 HEK-293T C454(1.12)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C63(1.16)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C454(1.05)  LDD1589  [9]
 LDCM0387  CL117 HEK-293T C454(1.07)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C63(0.97)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C454(1.12)  LDD1593  [9]
 LDCM0392  CL121 HEK-293T C454(1.11)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C63(1.08)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C454(0.96)  LDD1598  [9]
 LDCM0396  CL125 HEK-293T C454(1.13)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C63(1.17)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C454(0.95)  LDD1602  [9]
 LDCM0400  CL13 HEK-293T C454(1.07)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C63(1.04)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C454(1.23)  LDD1606  [9]
 LDCM0404  CL17 HEK-293T C63(1.59)  LDD1608  [9]
 LDCM0405  CL18 HEK-293T C63(0.91)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C63(1.03); C454(1.07)  LDD1610  [9]
 LDCM0407  CL2 HEK-293T C63(1.05)  LDD1611  [9]
 LDCM0409  CL21 HEK-293T C63(1.71)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C63(1.08)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C63(1.26)  LDD1615  [9]
 LDCM0413  CL25 HEK-293T C454(1.08)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C63(1.01)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C454(1.04)  LDD1619  [9]
 LDCM0417  CL29 HEK-293T C63(1.06)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C454(1.03)  LDD1622  [9]
 LDCM0419  CL30 HEK-293T C63(0.96)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C63(1.00); C454(1.20)  LDD1624  [9]
 LDCM0422  CL33 HEK-293T C63(1.39)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C63(1.01)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C63(1.12)  LDD1628  [9]
 LDCM0426  CL37 HEK-293T C454(0.97)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C454(0.91)  LDD1632  [9]
 LDCM0431  CL41 HEK-293T C63(1.03)  LDD1635  [9]
 LDCM0432  CL42 HEK-293T C63(1.00)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C63(0.95); C454(0.99)  LDD1637  [9]
 LDCM0435  CL45 HEK-293T C63(1.23)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C63(0.99)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C63(1.14)  LDD1641  [9]
 LDCM0439  CL49 HEK-293T C454(1.16)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C63(1.05)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C63(1.06)  LDD1645  [9]
 LDCM0444  CL53 HEK-293T C63(1.44)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C63(1.23)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C63(1.00); C454(0.92)  LDD1649  [9]
 LDCM0448  CL57 HEK-293T C63(1.29)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C63(1.07)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C63(0.95)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C63(1.17)  LDD1654  [9]
 LDCM0453  CL61 HEK-293T C454(1.08)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C63(0.97)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C454(0.92)  LDD1658  [9]
 LDCM0457  CL65 HEK-293T C63(1.07)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C63(1.10)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C63(0.97); C454(1.18)  LDD1662  [9]
 LDCM0461  CL69 HEK-293T C63(1.02)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C63(1.11); C454(0.97)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C63(1.10)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C63(1.06)  LDD1667  [9]
 LDCM0466  CL73 HEK-293T C454(0.98)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C63(1.01)  LDD1670  [9]
 LDCM0470  CL77 HEK-293T C63(1.19)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C63(0.99)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C63(0.96); C454(0.93)  LDD1675  [9]
 LDCM0475  CL81 HEK-293T C63(1.02)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C63(0.99)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C63(0.93)  LDD1680  [9]
 LDCM0479  CL85 HEK-293T C454(1.17)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C63(1.02)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C454(1.02)  LDD1684  [9]
 LDCM0483  CL89 HEK-293T C63(1.03)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C63(0.97)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C63(1.57)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C63(0.93); C454(1.24)  LDD1689  [9]
 LDCM0488  CL93 HEK-293T C63(1.18)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C63(1.03)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C63(1.30)  LDD1693  [9]
 LDCM0492  CL97 HEK-293T C454(0.98)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C63(1.03)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C454(1.09)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C454(1.08)  LDD1698  [9]
 LDCM0468  Fragment33 HEK-293T C454(0.93)  LDD1671  [9]
 LDCM0427  Fragment51 HEK-293T C63(0.98)  LDD1631  [9]
 LDCM0022  KB02 HEK-293T C454(0.66); C63(0.85)  LDD1492  [9]
 LDCM0023  KB03 HEK-293T C454(0.91); C63(1.05)  LDD1497  [9]
 LDCM0024  KB05 MKN45 C477(1.70)  LDD3332  [1]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C189(0.66)  LDD2150  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT) CDP-alcohol phosphatidyltransferase class-I family O14735
Inositol-trisphosphate 3-kinase B (ITPKB) Inositol phosphokinase (IPK) family P27987
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor SOX-7 (SOX7) . Q9BT81

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402