Details of the Target

General Information of Target

Target ID LDTP08887
Target Name Serine/threonine-protein kinase DCLK2 (DCLK2)
Gene Name DCLK2
Gene ID 166614
Synonyms
DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; EC 2.7.11.1; CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSSSGPKGNGLIPSPAHSAHCSFYRTR
TLQALSSEKKAKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGV
RTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKNINPNWSVNIKGGTSRALAAA
SSVKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKKTAHSFEQVLTDITEAIKLDSG
VVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDDFVLDHSECRVLKSSYSRSSA
VKYSGSKSPGPSRRSKSPASVNGTPSSQLSTPKSTKSSSSSPTSPGSFRGLKQISAHGRS
SSNVNGGPELDRCISPEGVNGNRCSESSTLLEKYKIGKVIGDGNFAVVKECIDRSTGKEF
ALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETATELFLVMELVKGGDLFDA
ITSSTKYTERDGSAMVYNLANALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGL
ATVVEGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRSENNLQED
LFDQILAGKLEFPAPYWDNITDSAKELISQMLQVNVEARCTAGQILSHPWVSDDASQENN
MQAEVTGKLKQHFNNALPKQNSTTTGVSVIMNTALDKEGQIFCSKHCQDSGRPGMEPISP
VPPSVEEIPVPGEAVPAPTPPESPTPHPPPAAPGGERAGTWRRHRD
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Subcellular location
Cytoplasm, cytoskeleton
Function
Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm.
TTD ID
T63298
Uniprot ID
Q8N568
DrugMap ID
TTHK3MO
Ensemble ID
ENST00000296550.12
HGNC ID
HGNC:19002
ChEMBL ID
CHEMBL5519

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA Deletion: p.H747TfsTer153 .
CCSW1 SNV: p.S31P .
G415 SNV: p.S385P .
HCT15 SNV: p.K85E .
HT SNV: p.V523G .
HT115 SNV: p.K243N .
IPC298 SNV: p.G381E .
MCC13 SNV: p.P736S
Substitution: p.G755N		 .
MDAMB453 SNV: p.N113K .
MEWO SNV: p.S334F .
MFE319 Deletion: p.K219RfsTer10 .
NALM6 Deletion: p.H747TfsTer153 .
NCIH1793 SNV: p.H435Q .
PC14 SNV: p.A737G .
PC9 SNV: p.A737G .
SW756 SNV: p.D600Y .
ZR751 SNV: p.R414K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
DBIA
 Probe Info  		C720(2.74)  LDD3417  [2]
IPM
 Probe Info  		N.A.  LDD0147  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C246(1.02)  LDD1509  [4]
 LDCM0237  AC12 HEK-293T C384(0.99)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C384(0.93)  LDD1512  [4]
 LDCM0278  AC19 HEK-293T C246(1.07)  LDD1517  [4]
 LDCM0280  AC20 HEK-293T C384(1.19)  LDD1519  [4]
 LDCM0282  AC22 HEK-293T C384(0.96)  LDD1521  [4]
 LDCM0287  AC27 HEK-293T C246(1.02)  LDD1526  [4]
 LDCM0288  AC28 HEK-293T C384(1.20)  LDD1527  [4]
 LDCM0290  AC3 HEK-293T C246(1.05)  LDD1529  [4]
 LDCM0291  AC30 HEK-293T C384(1.12)  LDD1530  [4]
 LDCM0296  AC35 HEK-293T C246(1.00)  LDD1535  [4]
 LDCM0297  AC36 HEK-293T C384(0.97)  LDD1536  [4]
 LDCM0299  AC38 HEK-293T C384(1.04)  LDD1538  [4]
 LDCM0301  AC4 HEK-293T C384(1.05)  LDD1540  [4]
 LDCM0305  AC43 HEK-293T C246(1.03)  LDD1544  [4]
 LDCM0306  AC44 HEK-293T C384(1.34)  LDD1545  [4]
 LDCM0308  AC46 HEK-293T C384(1.02)  LDD1547  [4]
 LDCM0314  AC51 HEK-293T C246(0.97)  LDD1553  [4]
 LDCM0315  AC52 HEK-293T C384(1.16)  LDD1554  [4]
 LDCM0317  AC54 HEK-293T C384(0.89)  LDD1556  [4]
 LDCM0322  AC59 HEK-293T C246(0.95)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C384(1.00)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C384(1.16)  LDD1563  [4]
 LDCM0326  AC62 HEK-293T C384(0.96)  LDD1565  [4]
 LDCM0156  Aniline NCI-H1299 13.65  LDD0403  [1]
 LDCM0368  CL10 HEK-293T C384(0.83)  LDD1572  [4]
 LDCM0406  CL19 HEK-293T C246(1.33)  LDD1610  [4]
 LDCM0408  CL20 HEK-293T C384(1.11)  LDD1612  [4]
 LDCM0410  CL22 HEK-293T C384(0.83)  LDD1614  [4]
 LDCM0420  CL31 HEK-293T C246(1.05)  LDD1624  [4]
 LDCM0421  CL32 HEK-293T C384(0.93)  LDD1625  [4]
 LDCM0423  CL34 HEK-293T C384(0.91)  LDD1627  [4]
 LDCM0433  CL43 HEK-293T C246(1.10)  LDD1637  [4]
 LDCM0434  CL44 HEK-293T C384(1.01)  LDD1638  [4]
 LDCM0436  CL46 HEK-293T C384(0.82)  LDD1640  [4]
 LDCM0446  CL55 HEK-293T C246(1.14)  LDD1649  [4]
 LDCM0447  CL56 HEK-293T C384(1.22)  LDD1650  [4]
 LDCM0449  CL58 HEK-293T C384(1.22)  LDD1652  [4]
 LDCM0459  CL67 HEK-293T C246(1.11)  LDD1662  [4]
 LDCM0460  CL68 HEK-293T C384(1.11)  LDD1663  [4]
 LDCM0462  CL7 HEK-293T C246(1.17)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C384(1.08)  LDD1666  [4]
 LDCM0472  CL79 HEK-293T C246(1.18)  LDD1675  [4]
 LDCM0473  CL8 HEK-293T C384(1.12)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C384(1.47)  LDD1677  [4]
 LDCM0476  CL82 HEK-293T C384(0.90)  LDD1679  [4]
 LDCM0486  CL91 HEK-293T C246(1.07)  LDD1689  [4]
 LDCM0487  CL92 HEK-293T C384(1.12)  LDD1690  [4]
 LDCM0489  CL94 HEK-293T C384(0.85)  LDD1692  [4]
 LDCM0022  KB02 HEK-293T C288(0.96); C246(0.96)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C288(0.94); C246(0.91)  LDD1497  [4]
 LDCM0024  KB05 SF-295 C720(2.74)  LDD3417  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kelch-like protein 15 (KLHL15) . Q96M94

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Fostamatinib Small molecular drug DB12010
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Xmd8-92 Small molecular drug D0M0QQ

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402