Details of the Target

General Information of Target

Target ID LDTP08741
Target Name Mesoderm induction early response protein 1 (MIER1)
Gene Name MIER1
Gene ID 57708
Synonyms
KIAA1610; Mesoderm induction early response protein 1; Early response 1; Er1; Mi-er1; hMi-er1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAR
EGDMPIHELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKD
SSGQEDETQSSNDDPSQSVASQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKK
EIMVGSMFQAEIPVGICRYKENEKVYENDDQLLWDPEYLPEDKVIIFLKDASRRTGDEKG
VEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKAAREELSVWTEEECRNFEQGL
KAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKYNLHPGVTDYM
DRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGEILNKEEVK
VEGLHINGPTGGNKKPLHADMDTNGYETDNLTTDPKLAHMTARNENDFDEKSERPAKRRR
VNSNGKESPGSSEFFQEAVSHGKFEELENTDD
Target Bioclass
Transcription factor
Subcellular location
Cytoplasm; Nucleus
Function
Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.
Uniprot ID
Q8N108
Ensemble ID
ENST00000355356.3
HGNC ID
HGNC:29657

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y218(12.71)  LDD3495  [1]
DBIA
 Probe Info  		C316(1.52)  LDD3346  [2]
SAHA-CA-4PAP
 Probe Info  		11.30  LDD0269  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
ATP probe
 Probe Info  		K305(0.00); K349(0.00); K331(0.00); K332(0.00)  LDD0199  [5]
m-APA
 Probe Info  		H67(0.00); H249(0.00); H425(0.00)  LDD2231  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		C263(0.00); C293(0.00)  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		C263(0.00); C293(0.00); C197(0.00)  LDD0037  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
SAHA-CA-8PAP
 Probe Info  		N.A.  LDD0362  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [7]
 LDCM0022  KB02 CDX: HA1; Afatinib resistant C250(1.04)  LDD2346  [2]
 LDCM0023  KB03 DU145 C316(2.67)  LDD2735  [2]
 LDCM0024  KB05 NCI-H1666 C316(1.52)  LDD3346  [2]
 LDCM0096  SAHA K562 11.30  LDD0269  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone deacetylase 1 (HDAC1) Histone deacetylase family Q13547
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) Peptidase M16 family P31930
Serine/threonine-protein kinase N1 (PKN1) AGC Ser/Thr protein kinase family Q16512
DNA-directed RNA polymerase II subunit RPB1 (POLR2A) RNA polymerase beta' chain family P24928
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Advanced glycosylation end product-specific receptor (AGER) . Q15109
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ezrin (EZR) . P15311
TAR DNA-binding protein 43 (TARDBP) . Q13148
Transmembrane protein 171 (TMEM171) . Q8WVE6

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264