Details of the Target

General Information of Target

Target ID LDTP08483
Target Name DENN domain-containing protein 1C (DENND1C)
Gene Name DENND1C
Gene ID 79958
Synonyms
FAM31C; DENN domain-containing protein 1C; Connecdenn 3; Protein FAM31C
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MESRAEGGSPAVFDWFFEAACPASLQEDPPILRQFPPDFRDQEAMQMVPKFCFPFDVERE
PPSPAVQHFTFALTDLAGNRRFGFCRLRAGTQSCLCILSHLPWFEVFYKLLNTVGDLLAQ
DQVTEAEELLQNLFQQSLSGPQASVGLELGSGVTVSSGQGIPPPTRGNSKPLSCFVAPDS
GRLPSIPENRNLTELVVAVTDENIVGLFAALLAERRVLLTASKLSTLTSCVHASCALLYP
MRWEHVLIPTLPPHLLDYCCAPMPYLIGVHASLAERVREKALEDVVVLNVDANTLETTFN
DVQALPPDVVSLLRLRLRKVALAPGEGVSRLFLKAQALLFGGYRDALVCSPGQPVTFSEE
VFLAQKPGAPLQAFHRRAVHLQLFKQFIEARLEKLNKGEGFSDQFEQEITGCGASSGALR
SYQLWADNLKKGGGALLHSVKAKTQPAVKNMYRSAKSGLKGVQSLLMYKDGDSVLQRGGS
LRAPALPSRSDRLQQRLPITQHFGKNRPLRPSRRRQLEEGTSEPPGAGTPPLSPEDEGCP
WAEEALDSSFLGSGEELDLLSEILDSLSMGAKSAGSLRPSQSLDCCHRGDLDSCFSLPNI
PRWQPDDKKLPEPEPQPLSLPSLQNASSLDATSSSKDSRSQLIPSESDQEVTSPSQSSTA
SADPSIWGDPKPSPLTEPLILHLTPSHKAAEDSTAQENPTPWLSTAPTEPSPPESPQILA
PTKPNFDIAWTSQPLDPSSDPSSLEDPRARPPKALLAERAHLQPREEPGALNSPATPTSN
CQKSQPSSRPRVADLKKCFEG
Target Bioclass
Other
Subcellular location
Cytoplasm, cytosol
Function Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
Uniprot ID
Q8IV53
Ensemble ID
ENST00000381480.7
HGNC ID
HGNC:26225

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HEC1 SNV: p.E399D .
HUH7 SNV: p.D56E .
MOLT4 SNV: p.R242C .
NALM6 SNV: p.R453H DBIA    Probe Info 
NCIH196 SNV: p.E202A .
NCIH716 SNV: p.R344H; p.Q445H .
SKN SNV: p.A545T .
TYKNU SNV: p.E523G .
WM2664 Insertion: p.A760GfsTer35 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C412(102.22)  LDD0209  [1]
IA-alkyne
 Probe Info  		C21(0.00); C52(0.00); C349(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C52(0.00); C349(0.00)  LDD0037  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C781(1.92)  LDD2187  [3]
 LDCM0572  Fragment10 Ramos C174(4.96)  LDD1466  [4]
 LDCM0573  Fragment11 Ramos C781(20.00)  LDD2190  [3]
 LDCM0574  Fragment12 Ramos C781(1.60)  LDD2191  [3]
 LDCM0575  Fragment13 Ramos C781(1.00)  LDD2192  [3]
 LDCM0576  Fragment14 Ramos C781(0.81)  LDD2193  [3]
 LDCM0579  Fragment20 Ramos C781(1.23)  LDD2194  [3]
 LDCM0580  Fragment21 Ramos C781(0.85)  LDD2195  [3]
 LDCM0582  Fragment23 Ramos C781(0.94)  LDD2196  [3]
 LDCM0578  Fragment27 Ramos C781(1.21)  LDD2197  [3]
 LDCM0586  Fragment28 Ramos C781(0.58)  LDD2198  [3]
 LDCM0588  Fragment30 Ramos C781(1.64)  LDD2199  [3]
 LDCM0589  Fragment31 Ramos C781(0.98)  LDD2200  [3]
 LDCM0590  Fragment32 Ramos C781(2.55)  LDD2201  [3]
 LDCM0468  Fragment33 Ramos C781(1.40)  LDD2202  [3]
 LDCM0596  Fragment38 Ramos C781(0.71)  LDD2203  [3]
 LDCM0566  Fragment4 Ramos C781(1.62)  LDD2184  [3]
 LDCM0610  Fragment52 Ramos C781(1.83)  LDD2204  [3]
 LDCM0614  Fragment56 Ramos C781(1.37)  LDD2205  [3]
 LDCM0569  Fragment7 Ramos C781(1.55)  LDD2186  [3]
 LDCM0571  Fragment9 Ramos C781(2.26)  LDD2188  [3]
 LDCM0022  KB02 T cell C174(20.00)  LDD1703  [5]
 LDCM0023  KB03 Jurkat C412(102.22)  LDD0209  [1]
 LDCM0024  KB05 MOLM-13 C8(4.68)  LDD3333  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SNARE-associated protein Snapin (SNAPIN) SNAPIN family O95295

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
4 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840