Details of the Target

General Information of Target

Target ID LDTP08098
Target Name Protein sidekick-1 (SDK1)
Gene Name SDK1
Gene ID 221935
Synonyms
Protein sidekick-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARGARPSAAGGGGGGAEPPERAGPGRPRGSPPGRARPSLAPRPGPEPSRPRAAPETSGG
DTAGAGRCGGRRAAKLGPGRRGWWALLALQLHLLRALAQDDVAPYFKTEPGLPQIHLEGN
RLVLTCLAEGSWPLEFKWMRDDSELTTYSSEYKYIIPSLQKLDAGFYRCVVRNRMGALLQ
RKSEVQVAYMGSFMDTDQRKTVSQGRAAILNLLPITSYPRPQVTWFREGHKIIPSNRIAI
TLENQLVILATTTSDAGAYYVQAVNEKNGENKTSPFIHLSIARDVGTPETMAPTIVVPPG
NRSVVAGSSETTLECIASARPVEDLSVTWKRNGVRITSGLHSFGRRLTISNPTSADTGPY
VCEAALPGSAFEPARATAFLFIIEPPYFTAEPESRISAEVEETVDIGCQAMGVPLPTLQW
YKDAISISRLQNPRYKVLASGGLRIQKLRPEDSGIFQCFASNEGGEIQTHTYLDVTNIAP
VFTQRPVDTTVTDGMTAILRCEVSGAPKPAITWKRENHILASGSVRIPRFMLLESGGLQI
APVFIQDAGNYTCYAANTEGSLNASATLTVWNRTSIVHPPEDHVVIKGTTATLHCGATHD
PRVSLRYVWKKDNVALTPSSTSRIVVEKDGSLLISQTWSGDIGDYSCEIVSEGGNDSRMA
RLEVIELPHSPQNLLVSPNSSHSHAVVLSWVRPFDGNSPILYYIVELSENNSPWKVHLSN
VGPEMTGVTVSGLTPARTYQFRVCAVNEVGRGQYSAETSRLMLPEEPPSAPPKNIVASGR
TNQSIMVQWQPPPETEHNGVLRGYILRYRLAGLPGEYQQRNITSPEVNYCLVTDLIIWTQ
YEIQVAAYNGAGLGVFSRAVTEYTLQGVPTAPPQNVQTEAVNSTTIQFLWNPPPQQFING
INQGYKLLAWPADAPEAVTVVTIAPDFHGVHHGHITNLKKFTAYFTSVLCFTTPGDGPPS
TPQLVWTQEDKPGAVGHLSFTEILDTSLKVSWQEPLEKNGIITGYQISWEVYGRNDSRLT
HTLNSTTHEYKIQGLSSLTTYTIDVAAVTAVGTGLVTSSTISSGVPPDLPGAPSNLVISN
ISPRSATLQFRPGYDGKTSISRWIVEGQVGAIGDEEEWVTLYEEENEPDAQMLEIPNLTP
YTHYRFRMKQVNIVGPSPYSPSSRVIQTLQAPPDVAPTSVTVRTASETSLRLRWVPLPDS
QYNGNPESVGYRIKYWRSDLQSSAVAQVVSDRLEREFTIEELEEWMEYELQMQAFNAVGA
GPWSEVVRGRTRESVPSAAPENVSAEAVSSTQILLTWTSVPEQDQNGLILGYKILFRAKD
LDPEPRSHIVRGNHTQSALLAGLRKFVLYELQVLAFTRIGNGVPSTPLILERTKDDAPGP
PVRLVFPEVRLTSVRIVWQPPEEPNGIILGYQIAYRLASSSPHTFTTVEVGATVRQFTAT
DLAPESAYIFRLSAKTRQGWGEPLEATVITTEKRERPAPPRELLVPQAEVTARSLRLQWV
PGSDGASPIRYFTMQVRELPRGEWQTYSSSISHEATACVVDRLRPFTSYKLRLKATNDIG
DSDFSSETEAVTTLQDVPGEPPGSVSATPHTTSSVLIQWQPPRDESLNGLLQGYRIYYRE
LEYEAGSGTEAKTLKNPIALHAELTAQSSFKTVNSSSTSTMCELTHLKKYRRYEVIMTAY
NIIGESPASAPVEVFVGEAAPAMAPQNVQVTPLTASQLEVTWDPPPPESQNGNIQGYKIY
YWEADSQNETEKMKVLFLPEPVVRLKNLTSHTKYLVSISAFNAAGDGPKSDPQQGRTHQA
APGAPSFLAFSEITSTTLNVSWGEPAAANGILQGYRVVYEPLAPVQGVSKVVTVEVRGNW
QRWLKVRDLTKGVTYFFRVQARTITYGPELQANITAGPAEGSPGSPRDVLVTKSASELTL
QWTEGHSGDTPTTGYVIEARPSDEGLWDMFVKDIPRSATSYTLSLDKLRQGVTYEFRVVA
VNEAGYGEPSNPSTAVSAQVEAPFYEEWWFLLVMALSSLIVILLVVFALVLHGQNKKYKN
CSTGKGISTMEESVTLDNGGFAALELSSRHLNVKSTFSKKNGTRSPPRPSPGGLHYSDED
ICNKYNGAVLTESVSLKEKSADASESEATDSDYEDALPKHSFVNHYMSDPTYYNSWKRRA
QGRAPAPHRYEAVAGSEAGAQLHPVITTQSAGGVYTPAGPGARTPLTGFSSFV
Target Bioclass
Immunoglobulin
Family
Sidekick family
Subcellular location
Cell membrane
Function
Adhesion molecule that promotes lamina-specific synaptic connections in the retina. Expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions.
Uniprot ID
Q7Z5N4
Ensemble ID
ENST00000404826.7
HGNC ID
HGNC:19307

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.E709K .
A2780 SNV: p.S2155Ter .
BXPC3 SNV: p.A96E .
CAL148 SNV: p.S689F .
CAL62 SNV: p.T1022N .
CAL72 SNV: p.E826A .
CASKI SNV: p.T1919K .
CCK81 SNV: p.A564T .
CHL1 SNV: p.G779E .
EGI1 SNV: p.R2088L .
HCT116 SNV: p.Y472N .
HCT15 SNV: p.A1047D .
HLF SNV: p.R331K .
HT115 SNV: p.K200N; p.R573Q; p.T1039I; p.F1876L .
HT29 SNV: p.P1070H .
HUH1 SNV: p.Q539R .
JHH7 SNV: p.L91R .
KMS11 SNV: p.A1260D .
KYSE30 SNV: p.D695V .
LN18 SNV: p.G286D .
LNCaP clone FGC SNV: p.E648Ter DBIA    Probe Info 
LOXIMVI SNV: p.K2157R .
LS123 SNV: p.I1531T .
LS180 SNV: p.A1843T .
MDAMB468 SNV: p.G2208S .
MEC1 SNV: p.R346L .
MFE296 Deletion: p.P299RfsTer18
SNV: p.R1358H;p.G1613E		 .
MINO SNV: p.R1147Q .
MKN1 SNV: p.R227G .
MKN45 SNV: p.A177T .
NCIN87 SNV: p.K1789M .
NUGC3 SNV: p.A777T .
OVK18 Deletion: p.N1636TfsTer3 .
RAMOS SNV: p.T2204I .
REC1 Insertion: p.E2134dup .
RVH421 SNV: p.D970N .
SKMEL3 SNV: p.R429K .
SNGM SNV: p.Y2153C .
SNU1 SNV: p.V1385A .
SUPT1 Insertion: p.Y1094LfsTer2 .
SW620 SNV: p.L418R .
SW837 SNV: p.K231N .
TOV21G SNV: p.E1402D .
WM115 Substitution: p.V2173M .
WM2664 Substitution: p.V2173M .
YSCCC SNV: p.E724K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C744(1.27)  LDD3316  [1]
5E-2FA
 Probe Info  		N.A.  LDD2235  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [2]
NAIA_5
 Probe Info  		N.A.  LDD2225  [3]
YN-1
 Probe Info  		N.A.  LDD0447  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C744(0.91)  LDD1507  [5]
 LDCM0226  AC11 HEK-293T C744(1.05)  LDD1509  [5]
 LDCM0270  AC15 HEK-293T C744(1.00)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C744(0.98)  LDD1515  [5]
 LDCM0278  AC19 HEK-293T C744(0.87)  LDD1517  [5]
 LDCM0283  AC23 HEK-293T C744(1.01)  LDD1522  [5]
 LDCM0285  AC25 HEK-293T C744(0.88)  LDD1524  [5]
 LDCM0287  AC27 HEK-293T C744(1.00)  LDD1526  [5]
 LDCM0290  AC3 HEK-293T C744(1.04)  LDD1529  [5]
 LDCM0292  AC31 HEK-293T C744(1.07)  LDD1531  [5]
 LDCM0294  AC33 HEK-293T C744(0.77)  LDD1533  [5]
 LDCM0296  AC35 HEK-293T C744(1.05)  LDD1535  [5]
 LDCM0300  AC39 HEK-293T C744(1.05)  LDD1539  [5]
 LDCM0303  AC41 HEK-293T C744(0.96)  LDD1542  [5]
 LDCM0305  AC43 HEK-293T C744(1.06)  LDD1544  [5]
 LDCM0309  AC47 HEK-293T C744(1.13)  LDD1548  [5]
 LDCM0311  AC49 HEK-293T C744(0.90)  LDD1550  [5]
 LDCM0314  AC51 HEK-293T C744(1.04)  LDD1553  [5]
 LDCM0318  AC55 HEK-293T C744(1.05)  LDD1557  [5]
 LDCM0320  AC57 HEK-293T C744(0.97)  LDD1559  [5]
 LDCM0322  AC59 HEK-293T C744(1.05)  LDD1561  [5]
 LDCM0327  AC63 HEK-293T C744(1.09)  LDD1566  [5]
 LDCM0334  AC7 HEK-293T C744(1.08)  LDD1568  [5]
 LDCM0356  AKOS034007680 HEK-293T C744(0.97)  LDD1570  [5]
 LDCM0369  CL100 HEK-293T C744(1.04)  LDD1573  [5]
 LDCM0373  CL104 HEK-293T C744(1.03)  LDD1577  [5]
 LDCM0377  CL108 HEK-293T C744(0.92)  LDD1581  [5]
 LDCM0379  CL11 HEK-293T C744(1.21)  LDD1583  [5]
 LDCM0382  CL112 HEK-293T C744(0.96)  LDD1586  [5]
 LDCM0386  CL116 HEK-293T C744(0.99)  LDD1590  [5]
 LDCM0391  CL120 HEK-293T C744(0.97)  LDD1595  [5]
 LDCM0395  CL124 HEK-293T C744(0.87)  LDD1599  [5]
 LDCM0399  CL128 HEK-293T C744(1.07)  LDD1603  [5]
 LDCM0403  CL16 HEK-293T C744(1.14)  LDD1607  [5]
 LDCM0404  CL17 HEK-293T C744(0.94)  LDD1608  [5]
 LDCM0406  CL19 HEK-293T C744(1.26)  LDD1610  [5]
 LDCM0411  CL23 HEK-293T C744(1.25)  LDD1615  [5]
 LDCM0416  CL28 HEK-293T C744(0.97)  LDD1620  [5]
 LDCM0417  CL29 HEK-293T C744(1.05)  LDD1621  [5]
 LDCM0420  CL31 HEK-293T C744(1.06)  LDD1624  [5]
 LDCM0424  CL35 HEK-293T C744(1.02)  LDD1628  [5]
 LDCM0429  CL4 HEK-293T C744(0.92)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C744(1.11)  LDD1634  [5]
 LDCM0431  CL41 HEK-293T C744(0.99)  LDD1635  [5]
 LDCM0433  CL43 HEK-293T C744(1.11)  LDD1637  [5]
 LDCM0437  CL47 HEK-293T C744(1.14)  LDD1641  [5]
 LDCM0440  CL5 HEK-293T C744(1.11)  LDD1644  [5]
 LDCM0443  CL52 HEK-293T C744(1.02)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C744(0.95)  LDD1647  [5]
 LDCM0446  CL55 HEK-293T C744(1.16)  LDD1649  [5]
 LDCM0450  CL59 HEK-293T C744(1.30)  LDD1653  [5]
 LDCM0456  CL64 HEK-293T C744(1.07)  LDD1659  [5]
 LDCM0457  CL65 HEK-293T C744(0.89)  LDD1660  [5]
 LDCM0459  CL67 HEK-293T C744(1.10)  LDD1662  [5]
 LDCM0462  CL7 HEK-293T C744(1.23)  LDD1665  [5]
 LDCM0464  CL71 HEK-293T C744(1.06)  LDD1667  [5]
 LDCM0469  CL76 HEK-293T C744(1.13)  LDD1672  [5]
 LDCM0470  CL77 HEK-293T C744(1.10)  LDD1673  [5]
 LDCM0472  CL79 HEK-293T C744(1.14)  LDD1675  [5]
 LDCM0477  CL83 HEK-293T C744(1.10)  LDD1680  [5]
 LDCM0482  CL88 HEK-293T C744(0.97)  LDD1685  [5]
 LDCM0483  CL89 HEK-293T C744(1.13)  LDD1686  [5]
 LDCM0486  CL91 HEK-293T C744(1.09)  LDD1689  [5]
 LDCM0490  CL95 HEK-293T C744(0.96)  LDD1693  [5]
 LDCM0022  KB02 A-172 C744(1.64)  LDD2251  [1]
 LDCM0023  KB03 A498 C744(2.12)  LDD2676  [1]
 LDCM0024  KB05 MEL167 C744(1.27)  LDD3316  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402