Details of the Target

General Information of Target

Target ID LDTP07992
Target Name STE20-related kinase adapter protein alpha (STRADA)
Gene Name STRADA
Gene ID 92335
Synonyms
LYK5; STRAD; STE20-related kinase adapter protein alpha; STRAD alpha; STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKTNDASSESIASFSKQEVMS
SFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELH
VSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVL
KALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKV
LPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLL
DTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCL
QRNPDARPSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGLVTN
LEELEVDDWEF
Target Bioclass
Enzyme
Family
Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Subcellular location
Nucleus
Function
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Uniprot ID
Q7RTN6
Ensemble ID
ENST00000336174.12
HGNC ID
HGNC:30172
ChEMBL ID
CHEMBL1795198

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA Deletion: p.S335PfsTer40 .
HEC1 SNV: p.R215H .
HEC1B SNV: p.R215H .
HT115 SNV: p.D26E .
MFE319 SNV: p.S371N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C298(1.30)  LDD3376  [1]
ATP probe
 Probe Info  		N.A.  LDD0199  [2]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C298(1.33)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C298(1.08)  LDD1509  [4]
 LDCM0270  AC15 HEK-293T C298(1.16)  LDD1513  [4]
 LDCM0277  AC18 HEK-293T C298(0.85)  LDD1516  [4]
 LDCM0278  AC19 HEK-293T C298(1.04)  LDD1517  [4]
 LDCM0279  AC2 HEK-293T C298(1.36)  LDD1518  [4]
 LDCM0283  AC23 HEK-293T C298(1.42)  LDD1522  [4]
 LDCM0286  AC26 HEK-293T C298(1.29)  LDD1525  [4]
 LDCM0287  AC27 HEK-293T C298(1.05)  LDD1526  [4]
 LDCM0290  AC3 HEK-293T C298(1.25)  LDD1529  [4]
 LDCM0292  AC31 HEK-293T C298(0.95)  LDD1531  [4]
 LDCM0295  AC34 HEK-293T C298(1.08)  LDD1534  [4]
 LDCM0296  AC35 HEK-293T C298(1.07)  LDD1535  [4]
 LDCM0300  AC39 HEK-293T C298(0.90)  LDD1539  [4]
 LDCM0304  AC42 HEK-293T C298(1.10)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C298(1.15)  LDD1544  [4]
 LDCM0309  AC47 HEK-293T C298(1.19)  LDD1548  [4]
 LDCM0313  AC50 HEK-293T C298(1.12)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C298(1.17)  LDD1553  [4]
 LDCM0318  AC55 HEK-293T C298(1.38)  LDD1557  [4]
 LDCM0321  AC58 HEK-293T C298(1.08)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C298(1.08)  LDD1561  [4]
 LDCM0327  AC63 HEK-293T C298(1.33)  LDD1566  [4]
 LDCM0334  AC7 HEK-293T C298(0.96)  LDD1568  [4]
 LDCM0369  CL100 HEK-293T C298(1.14); C68(0.92)  LDD1573  [4]
 LDCM0373  CL104 HEK-293T C298(0.60); C68(0.96)  LDD1577  [4]
 LDCM0377  CL108 HEK-293T C298(1.11); C68(1.04)  LDD1581  [4]
 LDCM0379  CL11 HEK-293T C298(1.04)  LDD1583  [4]
 LDCM0382  CL112 HEK-293T C298(1.15); C68(0.95)  LDD1586  [4]
 LDCM0386  CL116 HEK-293T C298(0.88); C68(0.86)  LDD1590  [4]
 LDCM0391  CL120 HEK-293T C298(1.11); C68(1.04)  LDD1595  [4]
 LDCM0395  CL124 HEK-293T C298(0.95); C68(0.99)  LDD1599  [4]
 LDCM0399  CL128 HEK-293T C298(1.12); C68(1.03)  LDD1603  [4]
 LDCM0403  CL16 HEK-293T C298(1.10); C68(1.07)  LDD1607  [4]
 LDCM0405  CL18 HEK-293T C298(1.14)  LDD1609  [4]
 LDCM0406  CL19 HEK-293T C298(1.20)  LDD1610  [4]
 LDCM0411  CL23 HEK-293T C298(1.31)  LDD1615  [4]
 LDCM0416  CL28 HEK-293T C298(1.13); C68(0.97)  LDD1620  [4]
 LDCM0419  CL30 HEK-293T C298(0.97)  LDD1623  [4]
 LDCM0420  CL31 HEK-293T C298(1.33)  LDD1624  [4]
 LDCM0424  CL35 HEK-293T C298(0.96)  LDD1628  [4]
 LDCM0429  CL4 HEK-293T C298(0.92); C68(1.11)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C298(0.86); C68(0.91)  LDD1634  [4]
 LDCM0432  CL42 HEK-293T C298(1.02)  LDD1636  [4]
 LDCM0433  CL43 HEK-293T C298(1.10)  LDD1637  [4]
 LDCM0437  CL47 HEK-293T C298(0.95)  LDD1641  [4]
 LDCM0443  CL52 HEK-293T C298(0.91); C68(1.00)  LDD1646  [4]
 LDCM0445  CL54 HEK-293T C298(1.16)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C298(1.08)  LDD1649  [4]
 LDCM0450  CL59 HEK-293T C298(1.01)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C298(1.19)  LDD1654  [4]
 LDCM0456  CL64 HEK-293T C298(0.81); C68(0.86)  LDD1659  [4]
 LDCM0458  CL66 HEK-293T C298(1.11)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C298(1.22)  LDD1662  [4]
 LDCM0462  CL7 HEK-293T C298(1.11)  LDD1665  [4]
 LDCM0464  CL71 HEK-293T C298(0.90)  LDD1667  [4]
 LDCM0469  CL76 HEK-293T C298(0.95); C68(1.07)  LDD1672  [4]
 LDCM0471  CL78 HEK-293T C298(1.14)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C298(1.05)  LDD1675  [4]
 LDCM0477  CL83 HEK-293T C298(0.75)  LDD1680  [4]
 LDCM0482  CL88 HEK-293T C298(1.51); C68(1.07)  LDD1685  [4]
 LDCM0485  CL90 HEK-293T C298(1.61)  LDD1688  [4]
 LDCM0486  CL91 HEK-293T C298(1.20)  LDD1689  [4]
 LDCM0490  CL95 HEK-293T C298(1.59)  LDD1693  [4]
 LDCM0022  KB02 Daoy C298(1.46)  LDD2314  [1]
 LDCM0023  KB03 D283 Med C298(2.40)  LDD2729  [1]
 LDCM0024  KB05 ONS-76 C298(1.30)  LDD3376  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine/threonine-protein kinase STK11 (STK11) CAMK Ser/Thr protein kinase family Q15831
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calcium-binding protein 39 (CAB39) Mo25 family Q9Y376

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402