Details of the Target

General Information of Target

Target ID LDTP07051
Target Name Rab GTPase-activating protein 1-like (RABGAP1L)
Gene Name RABGAP1L
Gene ID 9910
Synonyms
HHL; KIAA0471; Rab GTPase-activating protein 1-like
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEVRASLQKVSGSSDSVATMNSEEFVLVPQYADDNSTKHEEKPQLKIVSNGDEQLEKAME
EILRDSEKRPSSLLVDCQSSSEISDHSFGDIPASQTNKPSLQLILDPSNTEISTPRPSSP
GGLPEEDSVLFNKLTYLGCMKVSSPRNEVEALRAMATMKSSSQYPFPVTLYVPNVPEGSV
RIIDQSSNVEIASFPIYKVLFCARGHDGTTESNCFAFTESSHGSEEFQIHVFSCEIKEAV
SRILYSFCTAFKRSSRQVSDVKDSVIPTPDSDVFTFSVSLEVKEDDGKGNFSPVPKDRDK
FYFKLKQGIEKKVVITVQQLSNKELAIERCFGMLLSPGRNVKNSDMHLLDMESMGKSYDG
RAYVITGMWNPNAPVFLALNEETPKDKQVYMTVAVDMVVTEVVEPVRFLLETVVRVYPAN
ERFWYFSRKTFTETFFMRLKQSEGKGHTNAGDAIYEVVSLQRESDKEEPVTPTSGGGPMS
PQDDEAEEESDNELSSGTGDVSKDCPEKILYSWGELLGKWHSNLGARPKGLSTLVKSGVP
EALRAEVWQLLAGCHDNQAMLDRYRILITKDSAQESVITRDIHRTFPAHDYFKDTGGDGQ
ESLYKICKAYSVYDEDIGYCQGQSFLAAVLLLHMPEEQAFCVLVKIMYDYGLRDLYRNNF
EDLHCKFYQLERLMQEQLPDLHSHFSDLNLEAHMYASQWFLTLFTAKFPLCMVFHIIDLL
LCEGLNIIFHVALALLKTSKEDLLQADFEGALKFFRVQLPKRYRAEENARRLMEQACNIK
VPTKKLKKYEKEYQTMRESQLQQEDPMDRYKFVYL
Target Bioclass
Other
Subcellular location
Cytoplasmic vesicle
Function
GTP-hydrolysis activating protein (GAP) for small GTPase RAB22A, converting active RAB22A-GTP to the inactive form RAB22A-GDP. Plays a role in endocytosis and intracellular protein transport. Recruited by ANK2 to phosphatidylinositol 3-phosphate (PI3P)-positive early endosomes, where it inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration.
Uniprot ID
Q5R372
Ensemble ID
ENST00000251507.8
HGNC ID
HGNC:24663

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C554(67.83); C330(13.18)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C554(0.00); C330(0.00); C139(0.00); C777(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C554(0.00); C330(0.00); C248(0.00); C665(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C554(0.00); C330(0.00); C777(0.00); C139(0.00)  LDD0037  [2]
IPM
 Probe Info  		N.A.  LDD0147  [3]
TFBX
 Probe Info  		C665(0.00); C777(0.00); C139(0.00); C202(0.00)  LDD0148  [3]
Acrolein
 Probe Info  		N.A.  LDD0217  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0198  Dimethyl Fumarate(DMF) T cell C248(5.85)  LDD0513  [5]
 LDCM0625  F8 Ramos C665(0.88); C248(0.87)  LDD2187  [6]
 LDCM0572  Fragment10 Ramos C665(1.16)  LDD2189  [6]
 LDCM0573  Fragment11 Ramos C665(2.78); C248(20.00); C554(0.35)  LDD2190  [6]
 LDCM0574  Fragment12 Ramos C665(1.72)  LDD2191  [6]
 LDCM0575  Fragment13 Ramos C665(0.95)  LDD2192  [6]
 LDCM0576  Fragment14 Ramos C665(0.71); C248(0.99); C554(1.50)  LDD2193  [6]
 LDCM0579  Fragment20 Ramos C665(1.36)  LDD2194  [6]
 LDCM0580  Fragment21 Ramos C665(0.96); C554(1.99)  LDD2195  [6]
 LDCM0582  Fragment23 Ramos C665(0.92)  LDD2196  [6]
 LDCM0578  Fragment27 Ramos C665(0.81); C554(0.97)  LDD2197  [6]
 LDCM0586  Fragment28 Ramos C665(0.24); C248(1.84); C554(0.77)  LDD2198  [6]
 LDCM0588  Fragment30 Ramos C665(0.73)  LDD2199  [6]
 LDCM0589  Fragment31 Ramos C665(0.94)  LDD2200  [6]
 LDCM0590  Fragment32 Ramos C665(1.74)  LDD2201  [6]
 LDCM0468  Fragment33 Ramos C665(1.07); C554(0.96)  LDD2202  [6]
 LDCM0596  Fragment38 Ramos C665(0.83); C554(1.20)  LDD2203  [6]
 LDCM0566  Fragment4 Ramos C665(1.05); C248(1.72); C554(1.04)  LDD2184  [6]
 LDCM0610  Fragment52 Ramos C665(1.04)  LDD2204  [6]
 LDCM0614  Fragment56 Ramos C665(0.93); C554(1.48)  LDD2205  [6]
 LDCM0569  Fragment7 Ramos C665(0.89); C248(1.58); C554(1.04)  LDD2186  [6]
 LDCM0022  KB02 HEK-293T C665(0.90); C77(1.10); C139(1.17); C554(1.00)  LDD1492  [7]
 LDCM0023  KB03 Jurkat C554(67.83); C330(13.18)  LDD0209  [1]
 LDCM0024  KB05 HEK-293T C665(0.87); C77(1.17); C139(1.06); C554(1.11)  LDD1502  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-6 (CASP6) Peptidase C14A family P55212
Histone-lysine N-methyltransferase MECOM (MECOM) . Q03112
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Uncharacterized protein C9orf43 (C9orf43) . Q8TAL5

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402