Details of the Target

General Information of Target

Target ID LDTP06781
Target Name TBC1 domain family member 25 (TBC1D25)
Gene Name TBC1D25
Gene ID 4943
Synonyms
OATL1; TBC1 domain family member 25
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKKCESFLPPEFRSFAVDPQI
TSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQL
RVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKV
QQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRY
LLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAHP
YYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMK
RLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAF
DDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADAGFGGHRGWPVRQRHMLRPAGGGGST
FEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLS
EPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPV
PPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVL
RRARALFADYLQSEVWDSEEGAEATAAS
Target Bioclass
Other
Subcellular location
Cytoplasm
Function Acts as a GTPase-activating protein specific for RAB33B. Involved in the regulation of autophagosome maturation, the process in which autophagosomes fuse with endosomes and lysosomes.
Uniprot ID
Q3MII6
Ensemble ID
ENST00000376771.9
HGNC ID
HGNC:8092

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.R234Q; p.P436L .
MCC13 Substitution: p.Q609Ter .
MDAMB453 SNV: p.D135H .
MEWO SNV: p.G93E; p.H210Y .
MOLT4 SNV: p.T295S .
NUGC3 SNV: p.E128A .
OVK18 Deletion: p.A682LfsTer26 .
PF382 SNV: p.G496R .
RKO SNV: p.R294H .
SKMEL3 SNV: p.Y321N .
SKNSH SNV: p.L208M .
TE4 SNV: p.G304R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K653(1.14); K80(9.44)  LDD0277  [1]
DBIA
 Probe Info  		C43(1.47)  LDD3410  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C406(0.79)  LDD1508  [3]
 LDCM0226  AC11 HEK-293T C406(1.00)  LDD1509  [3]
 LDCM0259  AC14 HEK-293T C406(0.94); C43(1.00)  LDD1512  [3]
 LDCM0277  AC18 HEK-293T C406(0.83)  LDD1516  [3]
 LDCM0278  AC19 HEK-293T C406(0.78)  LDD1517  [3]
 LDCM0279  AC2 HEK-293T C406(0.88)  LDD1518  [3]
 LDCM0282  AC22 HEK-293T C406(1.09); C43(0.96)  LDD1521  [3]
 LDCM0286  AC26 HEK-293T C406(0.95)  LDD1525  [3]
 LDCM0287  AC27 HEK-293T C406(0.82)  LDD1526  [3]
 LDCM0290  AC3 HEK-293T C406(0.77)  LDD1529  [3]
 LDCM0291  AC30 HEK-293T C406(1.13); C43(0.98)  LDD1530  [3]
 LDCM0295  AC34 HEK-293T C406(0.95)  LDD1534  [3]
 LDCM0296  AC35 HEK-293T C406(1.08)  LDD1535  [3]
 LDCM0299  AC38 HEK-293T C406(1.05); C43(1.06)  LDD1538  [3]
 LDCM0304  AC42 HEK-293T C406(0.81)  LDD1543  [3]
 LDCM0305  AC43 HEK-293T C406(1.09)  LDD1544  [3]
 LDCM0308  AC46 HEK-293T C406(0.83); C43(0.87)  LDD1547  [3]
 LDCM0313  AC50 HEK-293T C406(0.81)  LDD1552  [3]
 LDCM0314  AC51 HEK-293T C406(1.15)  LDD1553  [3]
 LDCM0317  AC54 HEK-293T C406(1.05); C43(1.00)  LDD1556  [3]
 LDCM0321  AC58 HEK-293T C406(0.92)  LDD1560  [3]
 LDCM0322  AC59 HEK-293T C406(0.98)  LDD1561  [3]
 LDCM0323  AC6 HEK-293T C406(0.83); C43(0.93)  LDD1562  [3]
 LDCM0326  AC62 HEK-293T C406(1.52); C43(1.17)  LDD1565  [3]
 LDCM0368  CL10 HEK-293T C406(0.85); C43(0.94)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C406(0.85)  LDD1573  [3]
 LDCM0371  CL102 HEK-293T C406(0.85)  LDD1575  [3]
 LDCM0373  CL104 HEK-293T C406(0.92)  LDD1577  [3]
 LDCM0375  CL106 HEK-293T C406(1.03)  LDD1579  [3]
 LDCM0377  CL108 HEK-293T C406(1.24)  LDD1581  [3]
 LDCM0380  CL110 HEK-293T C406(1.06)  LDD1584  [3]
 LDCM0382  CL112 HEK-293T C406(1.12)  LDD1586  [3]
 LDCM0384  CL114 HEK-293T C406(0.79)  LDD1588  [3]
 LDCM0386  CL116 HEK-293T C406(1.00)  LDD1590  [3]
 LDCM0388  CL118 HEK-293T C406(1.08)  LDD1592  [3]
 LDCM0391  CL120 HEK-293T C406(1.09)  LDD1595  [3]
 LDCM0393  CL122 HEK-293T C406(0.96)  LDD1597  [3]
 LDCM0395  CL124 HEK-293T C406(0.79)  LDD1599  [3]
 LDCM0397  CL126 HEK-293T C406(1.03)  LDD1601  [3]
 LDCM0399  CL128 HEK-293T C406(1.02)  LDD1603  [3]
 LDCM0401  CL14 HEK-293T C406(0.88)  LDD1605  [3]
 LDCM0403  CL16 HEK-293T C406(0.79)  LDD1607  [3]
 LDCM0405  CL18 HEK-293T C406(0.87)  LDD1609  [3]
 LDCM0406  CL19 HEK-293T C406(0.79)  LDD1610  [3]
 LDCM0407  CL2 HEK-293T C406(0.92)  LDD1611  [3]
 LDCM0410  CL22 HEK-293T C406(0.91); C43(1.31)  LDD1614  [3]
 LDCM0414  CL26 HEK-293T C406(0.84)  LDD1618  [3]
 LDCM0416  CL28 HEK-293T C406(0.82)  LDD1620  [3]
 LDCM0419  CL30 HEK-293T C406(0.80)  LDD1623  [3]
 LDCM0420  CL31 HEK-293T C406(1.06)  LDD1624  [3]
 LDCM0423  CL34 HEK-293T C406(1.05); C43(1.12)  LDD1627  [3]
 LDCM0429  CL4 HEK-293T C406(0.74)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C406(1.09)  LDD1634  [3]
 LDCM0432  CL42 HEK-293T C406(0.83)  LDD1636  [3]
 LDCM0433  CL43 HEK-293T C406(1.05)  LDD1637  [3]
 LDCM0436  CL46 HEK-293T C406(0.91); C43(1.40)  LDD1640  [3]
 LDCM0441  CL50 HEK-293T C406(1.15)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C406(0.87)  LDD1646  [3]
 LDCM0445  CL54 HEK-293T C406(0.70)  LDD1648  [3]
 LDCM0446  CL55 HEK-293T C406(1.18)  LDD1649  [3]
 LDCM0449  CL58 HEK-293T C406(0.90); C43(1.06)  LDD1652  [3]
 LDCM0451  CL6 HEK-293T C406(0.82)  LDD1654  [3]
 LDCM0454  CL62 HEK-293T C406(0.94)  LDD1657  [3]
 LDCM0456  CL64 HEK-293T C406(0.89)  LDD1659  [3]
 LDCM0458  CL66 HEK-293T C406(0.74)  LDD1661  [3]
 LDCM0459  CL67 HEK-293T C406(0.86)  LDD1662  [3]
 LDCM0462  CL7 HEK-293T C406(1.05)  LDD1665  [3]
 LDCM0463  CL70 HEK-293T C406(0.99); C43(1.47)  LDD1666  [3]
 LDCM0467  CL74 HEK-293T C406(1.05)  LDD1670  [3]
 LDCM0469  CL76 HEK-293T C406(0.85)  LDD1672  [3]
 LDCM0471  CL78 HEK-293T C406(0.90)  LDD1674  [3]
 LDCM0472  CL79 HEK-293T C406(0.68)  LDD1675  [3]
 LDCM0476  CL82 HEK-293T C406(0.95); C43(1.45)  LDD1679  [3]
 LDCM0480  CL86 HEK-293T C406(0.93)  LDD1683  [3]
 LDCM0482  CL88 HEK-293T C406(0.85)  LDD1685  [3]
 LDCM0485  CL90 HEK-293T C406(1.02)  LDD1688  [3]
 LDCM0486  CL91 HEK-293T C406(0.89)  LDD1689  [3]
 LDCM0489  CL94 HEK-293T C406(0.82); C43(1.07)  LDD1692  [3]
 LDCM0493  CL98 HEK-293T C406(0.93)  LDD1696  [3]
 LDCM0427  Fragment51 HEK-293T C406(1.02)  LDD1631  [3]
 LDCM0022  KB02 A-375 C43(1.41)  LDD2255  [2]
 LDCM0023  KB03 A-375 C43(1.51)  LDD2672  [2]
 LDCM0024  KB05 RPMI-7951 C43(1.47)  LDD3410  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402