Details of the Target

General Information of Target

Target ID LDTP05915
Target Name Eukaryotic translation initiation factor 4E-binding protein 1 (EIF4EBP1)
Gene Name EIF4EBP1
Gene ID 1978
Synonyms
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLM
ECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
Target Type
Literature-reported
Target Bioclass
Other
Family
EIF4E-binding protein family
Function
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
TTD ID
T84941
Uniprot ID
Q13541
DrugMap ID
TTKGEBL
Ensemble ID
ENST00000338825.5
HGNC ID
HGNC:3288
ChEMBL ID
CHEMBL3351214

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-P2
 Probe Info  		6.44  LDD0348  [1]
STPyne
 Probe Info  		K57(0.70)  LDD0277  [2]
m-APA
 Probe Info  		14.08  LDD0403  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
TFBX
 Probe Info  		N.A.  LDD0027  [6]
WYneN
 Probe Info  		N.A.  LDD0021  [7]
ENE
 Probe Info  		C7(0.00); C62(0.00)  LDD0006  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0151  [8]
IPM
 Probe Info  		C62(0.00); C7(0.00)  LDD0005  [7]
VSF
 Probe Info  		N.A.  LDD0007  [7]
Phosphinate-6
 Probe Info  		C62(0.00); C7(0.00)  LDD0018  [9]
1d-yne
 Probe Info  		N.A.  LDD0357  [10]
Acrolein
 Probe Info  		N.A.  LDD0217  [11]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [11]
Methacrolein
 Probe Info  		N.A.  LDD0218  [11]
NAIA_5
 Probe Info  		N.A.  LDD2223  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 14.08  LDD0403  [3]
 LDCM0108  Chloroacetamide HeLa C62(0.00); H97(0.00)  LDD0222  [11]
 LDCM0632  CL-Sc Hep-G2 C7(1.14)  LDD2227  [12]
 LDCM0634  CY-0357 Hep-G2 C7(0.65)  LDD2228  [12]
 LDCM0107  IAA HeLa H97(0.00); C62(0.00)  LDD0221  [11]
 LDCM0109  NEM HeLa N.A.  LDD0223  [11]
 LDCM0627  NUDT7-COV-1 HEK-293T C7(0.98)  LDD2206  [13]
 LDCM0628  OTUB2-COV-1 HEK-293T C7(0.55)  LDD2207  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein argonaute-2 (AGO2) Argonaute family Q9UKV8
Serine/threonine-protein kinase mTOR (MTOR) PI3/PI4-kinase family P42345
Transcription factor
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720
POU domain, class 6, transcription factor 2 (POU6F2) POU transcription factor family P78424
Homeobox protein MOX-1 (MEOX1) . P50221
Proto-oncogene c-Rel (REL) . Q04864
Transcription factor 4 (TCF4) . P15884
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA damage-inducible transcript 4-like protein (DDIT4L) DDIT4 family Q96D03
Eukaryotic translation initiation factor 4E (EIF4E) Eukaryotic initiation factor 4E family P06730
Eukaryotic translation initiation factor 4E type 1B (EIF4E1B) Eukaryotic initiation factor 4E family A6NMX2
Regulatory-associated protein of mTOR (RPTOR) WD repeat RAPTOR family Q8N122
PRKCA-binding protein (PICK1) . Q9NRD5

References

1 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
9 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
10 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
11 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
12 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
13 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.