Details of the Target

General Information of Target

Target ID LDTP05542
Target Name Alpha-actinin-3 (ACTN3)
Gene Name ACTN3
Gene ID 89
Synonyms
Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLR
KAGTQIENIEEDFRNGLKLMLLLEVISGERLPRPDKGKMRFHKIANVNKALDFIASKGVK
LVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNV
QNFHTSWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKMLDAED
IVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASEL
LEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRRLHKPPRIQEKCQLEINFNTLQTKLR
LSHRPAFMPSEGKLVSDIANAWRGLEQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLH
EAWTRGKEEMLSQRDYDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDY
HEAASVNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDRLQLEFARRAAPFNNWLD
GAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGIQGEIQKICQTYGLRP
CSTNPYITLSPQDINTKWDMVRKLVPSCDQTLQEELARQQVNERLRRQFAAQANAIGPWI
QAKVEEVGRLAAGLAGSLEEQMAGLRQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHT
VYSMEHIRVGWEQLLTSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKQNGMM
EPDDFRACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTTEQVVA
SFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGALDYVAFSSALYGESD
L
Target Bioclass
Other
Family
Alpha-actinin family
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Uniprot ID
Q08043
Ensemble ID
ENST00000513398.2
HGNC ID
HGNC:165

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-9
 Probe Info  		E162(10.00); D112(1.02)  LDD2208  [1]
DBIA
 Probe Info  		C869(1.11)  LDD3380  [2]
AHL-Pu-1
 Probe Info  		C168(2.26)  LDD0168  [3]
HHS-475
 Probe Info  		Y229(1.01)  LDD0264  [4]
HHS-465
 Probe Info  		Y229(1.88)  LDD2237  [5]
TER-AC
 Probe Info  		N.A.  LDD0426  [6]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe3
 Probe Info  		11.37  LDD0464  [7]
STS-1
 Probe Info  		1.33  LDD0137  [8]
STS-2
 Probe Info  		N.A.  LDD0138  [8]
DA-2
 Probe Info  		N.A.  LDD0071  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C168(2.26)  LDD0168  [3]
 LDCM0116  HHS-0101 DM93 Y229(1.01)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y229(2.56)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y229(0.84)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y229(1.09)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y229(1.74)  LDD0268  [4]
 LDCM0022  KB02 A204 C869(1.65)  LDD2252  [2]
 LDCM0023  KB03 A2780 C869(5.43)  LDD2671  [2]
 LDCM0024  KB05 OV-90 C869(1.11)  LDD3380  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Propionyl-CoA carboxylase beta chain, mitochondrial (PCCB) AccD/PCCB family P05166
Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) Acyl-CoA dehydrogenase family P26440
Putative hydrolase DDAH2 (DDAH2) DDAH family O95865
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Mitochondrial inner membrane protease ATP23 homolog (ATP23) Peptidase M76 family Q9Y6H3
Protein-tyrosine kinase 6 (PTK6) Tyr protein kinase family Q13882
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (CTDSP1) . Q9GZU7
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
Tripartite motif-containing protein 29 (TRIM29) . Q14134
Transcription factor
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein GLIS2 (GLIS2) GLI C2H2-type zinc-finger protein family Q9BZE0
Zinc finger protein 343 (ZNF343) Krueppel C2H2-type zinc-finger protein family Q6P1L6
POU domain, class 6, transcription factor 2 (POU6F2) POU transcription factor family P78424
Zinc finger protein SNAI1 (SNAI1) Snail C2H2-type zinc-finger protein family O95863
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (SOHLH1) . Q5JUK2
Zinc finger protein 426 (ZNF426) . Q9BUY5
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myotilin (MYOT) Myotilin/palladin family Q9UBF9
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
C-X-C motif chemokine 5 (CXCL5) Intercrine alpha (chemokine CxC) family P42830
Other
Click To Hide/Show 21 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Acyl carrier protein, mitochondrial (NDUFAB1) Acyl carrier protein (ACP) family O14561
Alpha-actinin-1 (ACTN1) Alpha-actinin family P12814
Alpha-actinin-3 (ACTN3) Alpha-actinin family Q08043
Alpha-actinin-4 (ACTN4) Alpha-actinin family O43707
Ankyrin repeat and SOCS box protein 9 (ASB9) Ankyrin SOCS box (ASB) family Q96DX5
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
Keratin, type II cytoskeletal 6C (KRT6C) Intermediate filament family P48668
Myozenin-1 (MYOZ1) Myozenin family Q9NP98
Ciliary microtubule associated protein 1B (CIMAP1B) ODF3 family A8MYP8
Cation channel sperm-associated targeting subunit tau (C2CD6) . Q53TS8
Ciliary microtubule inner protein 1 (CIMIP1) . Q9H1P6
Extracellular matrix protein 1 (ECM1) . Q16610
Heat shock factor 2-binding protein (HSF2BP) . O75031
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
Melanoma-associated antigen 6 (MAGEA6) . P43360
MICAL-like protein 2 (MICALL2) . Q8IY33
Phostensin (PPP1R18) . Q6NYC8
Receptor-transporting protein 5 (RTP5) . Q14D33
TNF receptor-associated factor 1 (TRAF1) . Q13077
Uncharacterized protein NKAPD1 (NKAPD1) . Q6ZUT1

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.