Details of the Target

General Information of Target

Target ID LDTP05225
Target Name Forkhead box protein O4 (FOXO4)
Gene Name FOXO4
Gene ID 4303
Synonyms
AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPGNENSATEAAAIIDLDPDFEPQSRPRSCTWPLPRPEIANQPSEPPEVEPDLGEKVHT
EGRSEPILLPSRLPEPAGGPQPGILGAVTGPRKGGSRRNAWGNQSYAELISQAIESAPEK
RLTLAQIYEWMVRTVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIKVHNEATGKSSWWML
NPEGGKSGKAPRRRAASMDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSG
SPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTL
NEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCF
SSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLE
APGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLE
CDMDNIISDLMDEGEGLDFNFEPDP
Target Bioclass
Transcription factor
Subcellular location
Cytoplasm
Function
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Uniprot ID
P98177
Ensemble ID
ENST00000341558.3
HGNC ID
HGNC:7139

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAOV3 SNV: p.M474I .
HEC1B SNV: p.L160M .
P31FUJ SNV: p.P258Q .
RKO Insertion: p.L402AfsTer6 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C415(1.05)  LDD1507  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C415(1.05)  LDD1507  [1]
 LDCM0215  AC10 HEK-293T C415(1.09)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C415(1.20)  LDD1509  [1]
 LDCM0276  AC17 HEK-293T C415(0.96)  LDD1515  [1]
 LDCM0277  AC18 HEK-293T C415(1.07)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C415(1.28)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C415(1.07)  LDD1518  [1]
 LDCM0284  AC24 HEK-293T C415(1.00)  LDD1523  [1]
 LDCM0285  AC25 HEK-293T C415(1.08)  LDD1524  [1]
 LDCM0286  AC26 HEK-293T C415(1.04)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C415(1.02)  LDD1526  [1]
 LDCM0290  AC3 HEK-293T C415(1.08)  LDD1529  [1]
 LDCM0293  AC32 HEK-293T C415(0.97)  LDD1532  [1]
 LDCM0294  AC33 HEK-293T C415(1.08)  LDD1533  [1]
 LDCM0295  AC34 HEK-293T C415(1.11)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C415(1.16)  LDD1535  [1]
 LDCM0302  AC40 HEK-293T C415(0.99)  LDD1541  [1]
 LDCM0303  AC41 HEK-293T C415(1.09)  LDD1542  [1]
 LDCM0304  AC42 HEK-293T C415(1.13)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C415(1.03)  LDD1544  [1]
 LDCM0310  AC48 HEK-293T C415(1.03)  LDD1549  [1]
 LDCM0311  AC49 HEK-293T C415(0.97)  LDD1550  [1]
 LDCM0313  AC50 HEK-293T C415(1.02)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C415(1.09)  LDD1553  [1]
 LDCM0319  AC56 HEK-293T C415(0.98)  LDD1558  [1]
 LDCM0320  AC57 HEK-293T C415(1.16)  LDD1559  [1]
 LDCM0321  AC58 HEK-293T C415(1.05)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C415(1.14)  LDD1561  [1]
 LDCM0328  AC64 HEK-293T C415(0.96)  LDD1567  [1]
 LDCM0345  AC8 HEK-293T C415(1.11)  LDD1569  [1]
 LDCM0356  AKOS034007680 HEK-293T C415(1.04)  LDD1570  [1]
 LDCM0275  AKOS034007705 HEK-293T C415(0.94)  LDD1514  [1]
 LDCM0390  CL12 HEK-293T C415(1.17)  LDD1594  [1]
 LDCM0404  CL17 HEK-293T C415(1.15)  LDD1608  [1]
 LDCM0405  CL18 HEK-293T C415(1.02)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C415(1.19)  LDD1610  [1]
 LDCM0412  CL24 HEK-293T C415(1.14)  LDD1616  [1]
 LDCM0417  CL29 HEK-293T C415(1.09)  LDD1621  [1]
 LDCM0419  CL30 HEK-293T C415(1.12)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C415(1.18)  LDD1624  [1]
 LDCM0425  CL36 HEK-293T C415(1.09)  LDD1629  [1]
 LDCM0431  CL41 HEK-293T C415(1.08)  LDD1635  [1]
 LDCM0432  CL42 HEK-293T C415(1.14)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C415(1.10)  LDD1637  [1]
 LDCM0438  CL48 HEK-293T C415(1.15)  LDD1642  [1]
 LDCM0440  CL5 HEK-293T C415(1.09)  LDD1644  [1]
 LDCM0444  CL53 HEK-293T C415(1.08)  LDD1647  [1]
 LDCM0445  CL54 HEK-293T C415(1.09)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C415(1.22)  LDD1649  [1]
 LDCM0451  CL6 HEK-293T C415(1.08)  LDD1654  [1]
 LDCM0452  CL60 HEK-293T C415(1.15)  LDD1655  [1]
 LDCM0457  CL65 HEK-293T C415(1.10)  LDD1660  [1]
 LDCM0458  CL66 HEK-293T C415(1.05)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C415(1.12)  LDD1662  [1]
 LDCM0462  CL7 HEK-293T C415(1.11)  LDD1665  [1]
 LDCM0465  CL72 HEK-293T C415(1.21)  LDD1668  [1]
 LDCM0470  CL77 HEK-293T C415(1.04)  LDD1673  [1]
 LDCM0471  CL78 HEK-293T C415(1.06)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C415(1.06)  LDD1675  [1]
 LDCM0478  CL84 HEK-293T C415(1.03)  LDD1681  [1]
 LDCM0483  CL89 HEK-293T C415(1.04)  LDD1686  [1]
 LDCM0485  CL90 HEK-293T C415(1.27)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C415(1.19)  LDD1689  [1]
 LDCM0491  CL96 HEK-293T C415(0.91)  LDD1694  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
NAD-dependent protein deacetylase sirtuin-1 (SIRT1) Sirtuin family Q96EB6
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 4 (SMAD4) Dwarfin/SMAD family Q13485
Forkhead box protein O4 (FOXO4) . P98177
Proto-oncogene c-Rel (REL) . Q04864
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein sigma (SFN) 14-3-3 family P31947

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019