Details of the Target

General Information of Target

Target ID LDTP04769
Target Name Breast cancer anti-estrogen resistance protein 1 (BCAR1)
Gene Name BCAR1
Gene ID 9564
Synonyms
CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRL
KILVGMYDKKPAGPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTP
SKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQ
VPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHL
LAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPS
NHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAED
VYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA
PAEGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDL
AGSAGATGSWRSPSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAK
LSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGN
ASLLFRRTKATAPGPEGGGTLHPNPTDKTSSIQSRPLPSPPKFTSQDSPDGQYENSEGGW
MEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLKQFERLEQEVSRPIDHDLA
NWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATNQPPKIFVA
HSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSP
SAAQDMVERVKELGHSTQQFRRVLGQLAAA
Target Bioclass
Other
Family
CAS family
Subcellular location
Cell junction, focal adhesion
Function
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling.
Uniprot ID
P56945
Ensemble ID
ENST00000162330.10
HGNC ID
HGNC:971

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.R426C .
ABC1 SNV: p.V274F .
DETROIT562 SNV: p.K314M .
HCT116 Insertion: p.M272HfsTer17 .
MELHO SNV: p.P499T .
NCIH2286 Deletion: p.E445del .
SKMEL24 Substitution: p.P636F .
SKNSH SNV: p.Q228H .
SNGM Deletion: p.G567EfsTer135
SNV: .		 .
SUPT1 Deletion: p.G618VfsTer84 .
YSCCC SNV: p.K685N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.99  LDD0402  [1]
C-Sul
 Probe Info  		5.94  LDD0066  [2]
STPyne
 Probe Info  		K540(10.00); K831(9.98); K851(1.53)  LDD0277  [3]
Probe 1
 Probe Info  		Y12(11.18)  LDD3495  [4]
DBIA
 Probe Info  		C628(2.55)  LDD3342  [5]
HHS-475
 Probe Info  		Y267(2.78)  LDD0264  [6]
Acrolein
 Probe Info  		N.A.  LDD0225  [7]
5E-2FA
 Probe Info  		N.A.  LDD2235  [8]
IA-alkyne
 Probe Info  		C820(0.00); C582(0.00)  LDD0162  [9]
NAIA_4
 Probe Info  		N.A.  LDD2226  [10]
IPM
 Probe Info  		N.A.  LDD0147  [11]
TFBX
 Probe Info  		N.A.  LDD0148  [11]
AOyne
 Probe Info  		13.40  LDD0443  [12]
NAIA_5
 Probe Info  		C582(0.00); C46(0.00); C820(0.00)  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C820(0.95)  LDD1513  [13]
 LDCM0283  AC23 HEK-293T C820(0.86)  LDD1522  [13]
 LDCM0292  AC31 HEK-293T C820(1.08)  LDD1531  [13]
 LDCM0300  AC39 HEK-293T C820(0.81)  LDD1539  [13]
 LDCM0309  AC47 HEK-293T C820(0.88)  LDD1548  [13]
 LDCM0318  AC55 HEK-293T C820(0.94)  LDD1557  [13]
 LDCM0327  AC63 HEK-293T C820(1.16)  LDD1566  [13]
 LDCM0334  AC7 HEK-293T C820(0.89)  LDD1568  [13]
 LDCM0632  CL-Sc Hep-G2 C582(20.00); C820(1.91)  LDD2227  [10]
 LDCM0367  CL1 HEK-293T C820(0.91)  LDD1571  [13]
 LDCM0370  CL101 HEK-293T C820(0.94)  LDD1574  [13]
 LDCM0374  CL105 HEK-293T C820(0.99)  LDD1578  [13]
 LDCM0378  CL109 HEK-293T C820(0.82)  LDD1582  [13]
 LDCM0379  CL11 HEK-293T C820(1.07)  LDD1583  [13]
 LDCM0383  CL113 HEK-293T C820(1.15)  LDD1587  [13]
 LDCM0387  CL117 HEK-293T C820(0.94)  LDD1591  [13]
 LDCM0392  CL121 HEK-293T C820(1.22)  LDD1596  [13]
 LDCM0396  CL125 HEK-293T C820(0.85)  LDD1600  [13]
 LDCM0400  CL13 HEK-293T C820(0.96)  LDD1604  [13]
 LDCM0411  CL23 HEK-293T C820(0.94)  LDD1615  [13]
 LDCM0413  CL25 HEK-293T C820(1.08)  LDD1617  [13]
 LDCM0424  CL35 HEK-293T C820(0.90)  LDD1628  [13]
 LDCM0426  CL37 HEK-293T C820(1.01)  LDD1630  [13]
 LDCM0437  CL47 HEK-293T C820(0.98)  LDD1641  [13]
 LDCM0439  CL49 HEK-293T C820(0.85)  LDD1643  [13]
 LDCM0450  CL59 HEK-293T C820(0.94)  LDD1653  [13]
 LDCM0453  CL61 HEK-293T C820(1.12)  LDD1656  [13]
 LDCM0464  CL71 HEK-293T C820(1.10)  LDD1667  [13]
 LDCM0466  CL73 HEK-293T C820(0.95)  LDD1669  [13]
 LDCM0477  CL83 HEK-293T C820(0.99)  LDD1680  [13]
 LDCM0479  CL85 HEK-293T C820(0.83)  LDD1682  [13]
 LDCM0490  CL95 HEK-293T C820(1.12)  LDD1693  [13]
 LDCM0492  CL97 HEK-293T C820(0.90)  LDD1695  [13]
 LDCM0573  Fragment11 Ramos C820(0.79)  LDD2190  [14]
 LDCM0576  Fragment14 Ramos C820(2.21)  LDD2193  [14]
 LDCM0586  Fragment28 Ramos C820(2.02)  LDD2198  [14]
 LDCM0566  Fragment4 Ramos C820(4.75)  LDD2184  [14]
 LDCM0569  Fragment7 Ramos C820(7.26)  LDD2186  [14]
 LDCM0116  HHS-0101 DM93 Y267(2.78)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y267(7.97)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y267(0.84)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y267(1.67)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y267(1.71)  LDD0268  [6]
 LDCM0022  KB02 Ramos C820(5.89)  LDD2182  [14]
 LDCM0023  KB03 A-375 C628(3.10)  LDD2672  [5]
 LDCM0024  KB05 NCI-H1155 C628(2.55)  LDD3342  [5]
 LDCM0109  NEM HeLa N.A.  LDD0225  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 (INPPL1) Inositol 1,4,5-trisphosphate 5-phosphatase family O15357
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Matrix metalloproteinase-14 (MMP14) Peptidase M10A family P50281
Inactive tyrosine-protein kinase PEAK1 (PEAK1) Protein kinase superfamily Q9H792
Activated CDC42 kinase 1 (TNK2) Tyr protein kinase family Q07912
Focal adhesion kinase 1 (PTK2) Tyr protein kinase family Q05397
Proto-oncogene tyrosine-protein kinase Src (SRC) Tyr protein kinase family P12931
Tyrosine-protein kinase Fyn (FYN) Tyr protein kinase family P06241
Tyrosine-protein kinase Yes (YES1) Tyr protein kinase family P07947
Tyrosine-protein phosphatase non-receptor type 1 (PTPN1) Protein-tyrosine phosphatase family P18031
Tyrosine-protein phosphatase non-receptor type 12 (PTPN12) Protein-tyrosine phosphatase family Q05209
Tensin-3 (TNS3) PTEN phosphatase protein family Q68CZ2
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein GLIS2 (GLIS2) GLI C2H2-type zinc-finger protein family Q9BZE0
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Adapter molecule crk (CRK) CRK family P46108
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
SRC kinase signaling inhibitor 1 (SRCIN1) SRCIN1 family Q9C0H9
Coatomer subunit beta (COPB1) . P53618
Cytoplasmic protein NCK2 (NCK2) . O43639
PML-RARA-regulated adapter molecule 1 (PRAM1) . Q96QH2
Ras GTPase-activating-like protein IQGAP1 (IQGAP1) . P46940

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
13 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
14 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578