Details of the Target

General Information of Target

Target ID LDTP04125
Target Name Rap1 GTPase-activating protein 1 (RAP1GAP)
Gene Name RAP1GAP
Gene ID 5909
Synonyms
KIAA0474; RAP1GA1; Rap1 GTPase-activating protein 1; Rap1GAP; Rap1GAP1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTN
HEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDV
IGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRL
IVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFR
GGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDE
NTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPE
FQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMEN
GSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIV
PGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQ
SSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGT
PHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQ
LGC
Target Bioclass
Other
Subcellular location
Golgi apparatus membrane
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Uniprot ID
P47736
Ensemble ID
ENST00000374765.9
HGNC ID
HGNC:9858

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.T454I DBIA    Probe Info 
DU145 Deletion: p.D641TfsTer132 DBIA    Probe Info 
HEL SNV: p.S629T .
HEL9217 SNV: p.S629T .
LS123 SNV: p.S495P .
NCIH1155 SNV: p.A559V DBIA    Probe Info 
NCIH1944 SNV: p.S32C .
ONS76 Deletion: p.E581del .
SKNSH SNV: p.G446V .
SUPT1 SNV: p.G289W DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C83(17.44)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C83(0.00); C644(0.00); C146(0.00); C257(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C257(0.00); C83(0.00); C644(0.00); C161(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C83(0.00); C644(0.00); C376(0.00); C161(0.00)  LDD0037  [2]
IPM
 Probe Info  		N.A.  LDD0025  [3]
WYneN
 Probe Info  		C644(0.00); C83(0.00)  LDD0021  [4]
WYneO
 Probe Info  		N.A.  LDD0022  [4]
VSF
 Probe Info  		N.A.  LDD0007  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C644(0.97)  LDD1508  [5]
 LDCM0237  AC12 HEK-293T C644(0.91)  LDD1510  [5]
 LDCM0270  AC15 HEK-293T C83(1.20)  LDD1513  [5]
 LDCM0277  AC18 HEK-293T C644(0.96)  LDD1516  [5]
 LDCM0279  AC2 HEK-293T C644(1.08)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C644(0.90)  LDD1519  [5]
 LDCM0283  AC23 HEK-293T C83(1.24)  LDD1522  [5]
 LDCM0286  AC26 HEK-293T C644(1.04)  LDD1525  [5]
 LDCM0288  AC28 HEK-293T C644(0.85)  LDD1527  [5]
 LDCM0292  AC31 HEK-293T C83(1.04)  LDD1531  [5]
 LDCM0295  AC34 HEK-293T C644(1.15)  LDD1534  [5]
 LDCM0297  AC36 HEK-293T C644(0.80)  LDD1536  [5]
 LDCM0300  AC39 HEK-293T C83(1.42)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C644(0.92)  LDD1540  [5]
 LDCM0304  AC42 HEK-293T C644(1.09)  LDD1543  [5]
 LDCM0306  AC44 HEK-293T C644(0.95)  LDD1545  [5]
 LDCM0309  AC47 HEK-293T C83(1.04)  LDD1548  [5]
 LDCM0313  AC50 HEK-293T C644(1.02)  LDD1552  [5]
 LDCM0315  AC52 HEK-293T C644(0.93)  LDD1554  [5]
 LDCM0318  AC55 HEK-293T C83(1.32)  LDD1557  [5]
 LDCM0321  AC58 HEK-293T C644(1.05)  LDD1560  [5]
 LDCM0324  AC60 HEK-293T C644(0.85)  LDD1563  [5]
 LDCM0327  AC63 HEK-293T C83(1.22)  LDD1566  [5]
 LDCM0334  AC7 HEK-293T C83(1.21)  LDD1568  [5]
 LDCM0372  CL103 HEK-293T C644(1.11)  LDD1576  [5]
 LDCM0376  CL107 HEK-293T C644(1.08)  LDD1580  [5]
 LDCM0379  CL11 HEK-293T C83(1.51)  LDD1583  [5]
 LDCM0381  CL111 HEK-293T C644(1.05)  LDD1585  [5]
 LDCM0385  CL115 HEK-293T C644(1.02)  LDD1589  [5]
 LDCM0389  CL119 HEK-293T C644(0.94)  LDD1593  [5]
 LDCM0394  CL123 HEK-293T C644(1.19)  LDD1598  [5]
 LDCM0398  CL127 HEK-293T C644(0.87)  LDD1602  [5]
 LDCM0402  CL15 HEK-293T C644(1.16)  LDD1606  [5]
 LDCM0405  CL18 HEK-293T C644(1.13)  LDD1609  [5]
 LDCM0408  CL20 HEK-293T C644(0.98)  LDD1612  [5]
 LDCM0411  CL23 HEK-293T C83(1.97)  LDD1615  [5]
 LDCM0415  CL27 HEK-293T C644(1.15)  LDD1619  [5]
 LDCM0418  CL3 HEK-293T C644(0.90)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C644(1.11)  LDD1623  [5]
 LDCM0421  CL32 HEK-293T C644(0.91)  LDD1625  [5]
 LDCM0424  CL35 HEK-293T C83(1.50)  LDD1628  [5]
 LDCM0428  CL39 HEK-293T C644(1.08)  LDD1632  [5]
 LDCM0432  CL42 HEK-293T C644(1.05)  LDD1636  [5]
 LDCM0434  CL44 HEK-293T C644(0.88)  LDD1638  [5]
 LDCM0437  CL47 HEK-293T C83(1.24)  LDD1641  [5]
 LDCM0445  CL54 HEK-293T C644(1.03)  LDD1648  [5]
 LDCM0447  CL56 HEK-293T C644(1.00)  LDD1650  [5]
 LDCM0450  CL59 HEK-293T C83(1.46)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C644(0.98)  LDD1654  [5]
 LDCM0455  CL63 HEK-293T C644(0.97)  LDD1658  [5]
 LDCM0458  CL66 HEK-293T C644(1.18)  LDD1661  [5]
 LDCM0460  CL68 HEK-293T C644(0.95)  LDD1663  [5]
 LDCM0464  CL71 HEK-293T C83(1.31)  LDD1667  [5]
 LDCM0471  CL78 HEK-293T C644(1.18)  LDD1674  [5]
 LDCM0473  CL8 HEK-293T C644(0.90)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C644(0.97)  LDD1677  [5]
 LDCM0477  CL83 HEK-293T C83(1.55)  LDD1680  [5]
 LDCM0481  CL87 HEK-293T C644(0.90)  LDD1684  [5]
 LDCM0485  CL90 HEK-293T C644(1.00)  LDD1688  [5]
 LDCM0487  CL92 HEK-293T C644(0.94)  LDD1690  [5]
 LDCM0490  CL95 HEK-293T C83(3.34)  LDD1693  [5]
 LDCM0494  CL99 HEK-293T C644(1.32)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C644(1.03)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C644(0.82)  LDD1671  [5]
 LDCM0022  KB02 22RV1 C321(2.31); C83(2.41); C708(1.34)  LDD2243  [6]
 LDCM0023  KB03 Jurkat C83(17.44)  LDD0209  [1]
 LDCM0024  KB05 IGR37 C83(1.91)  LDD3314  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ras-related protein Rap-1b (RAP1B) Ras family P61224
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Axonemal dynein light intermediate polypeptide 1 (DNALI1) Inner dynein arm light chain family O14645

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840