Details of the Target

General Information of Target

Target ID LDTP03718
Target Name Tyrosine-protein phosphatase non-receptor type 7 (PTPN7)
Gene Name PTPN7
Gene ID 5778
Synonyms
Tyrosine-protein phosphatase non-receptor type 7; EC 3.1.3.48; Hematopoietic protein-tyrosine phosphatase; HEPTP; Protein-tyrosine phosphatase LC-PTP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVQAHGGRSRAQPLTLSLGAAMTQPPPEKTPAKKHVRLQERRGSNVALMLDVRSLGAVEP
ICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEFLKIPSNFVSPEDLDIPGHA
SKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGPMPNTVSDFW
EMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQY
QEERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGC
FIATRIGCQQLKARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class subfamily
Subcellular location
Cytoplasm
Function Protein phosphatase that acts preferentially on tyrosine-phosphorylated MAPK1. Plays a role in the regulation of T and B-lymphocyte development and signal transduction.
Uniprot ID
P35236
Ensemble ID
ENST00000309017.8
HGNC ID
HGNC:9659
ChEMBL ID
CHEMBL2219

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.L270I .
HT115 SNV: p.R124Q .
IM95 Deletion: p.R8AfsTer7 .
JURKAT SNV: p.Q241P Compound 10    Probe Info 
NCIH1155 SNV: p.V323M .
NCIH2172 Substitution: p.Q235R .
SHP77 SNV: p.E99K .
SNU1079 SNV: p.N151K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C62(459.81); C228(4.94)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C204(0.00); C300(0.00); C324(0.00); C62(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C324(0.00); C204(0.00); C228(0.00); C308(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C204(0.00); C228(0.00); C324(0.00); C308(0.00)  LDD0037  [2]
Compound 10
 Probe Info  		N.A.  LDD2216  [3]
Compound 11
 Probe Info  		N.A.  LDD2213  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C308(1.39); C300(1.35); C228(1.54); C62(2.17)  LDD2187  [5]
 LDCM0572  Fragment10 Ramos C308(0.39); C300(0.46); C228(0.79); C62(1.38)  LDD2189  [5]
 LDCM0573  Fragment11 Ramos C308(0.23); C300(0.80); C228(20.00); C62(0.33)  LDD2190  [5]
 LDCM0574  Fragment12 Ramos C308(0.34); C300(0.45); C228(0.58); C62(0.62)  LDD2191  [5]
 LDCM0575  Fragment13 Ramos C308(0.78); C300(1.12); C228(0.99); C62(0.89)  LDD2192  [5]
 LDCM0576  Fragment14 Ramos C308(0.68); C300(0.46); C228(0.74); C62(0.53)  LDD2193  [5]
 LDCM0579  Fragment20 Ramos C308(0.27); C300(0.41); C228(0.49); C62(0.57)  LDD2194  [5]
 LDCM0580  Fragment21 Ramos C308(0.63); C300(0.95); C228(1.08); C62(1.02)  LDD2195  [5]
 LDCM0582  Fragment23 Ramos C308(0.25); C300(0.34); C228(0.80); C62(1.04)  LDD2196  [5]
 LDCM0578  Fragment27 Ramos C308(0.77); C300(1.14); C228(1.08); C62(0.99)  LDD2197  [5]
 LDCM0586  Fragment28 Ramos C308(0.26); C300(0.69); C228(0.67); C62(0.76)  LDD2198  [5]
 LDCM0588  Fragment30 Ramos C308(0.85); C300(1.14); C228(1.11); C62(1.14)  LDD2199  [5]
 LDCM0589  Fragment31 Ramos C308(0.66); C300(1.02); C228(0.97); C62(0.78)  LDD2200  [5]
 LDCM0590  Fragment32 Ramos C308(0.21); C300(0.29); C228(0.68); C62(1.14)  LDD2201  [5]
 LDCM0468  Fragment33 Ramos C308(0.70); C300(0.87); C228(1.04); C62(0.95)  LDD2202  [5]
 LDCM0596  Fragment38 Ramos C308(0.69); C300(0.94); C228(0.83); C62(0.73)  LDD2203  [5]
 LDCM0566  Fragment4 Ramos C308(0.70); C300(1.02); C228(0.76); C62(1.03)  LDD2184  [5]
 LDCM0610  Fragment52 Ramos C308(0.87); C300(2.17); C228(0.92); C62(1.09)  LDD2204  [5]
 LDCM0614  Fragment56 Ramos C308(1.20); C300(1.25); C228(1.02); C62(1.20)  LDD2205  [5]
 LDCM0569  Fragment7 Ramos C308(0.45); C300(0.53); C228(0.67); C62(1.42)  LDD2186  [5]
 LDCM0571  Fragment9 Ramos C308(0.40); C300(0.52); C228(0.66); C62(0.66)  LDD2188  [5]
 LDCM0022  KB02 Ramos C308(0.33); C300(0.35); C228(0.75); C62(2.13)  LDD2182  [5]
 LDCM0023  KB03 Jurkat C62(459.81); C228(4.94)  LDD0209  [1]
 LDCM0024  KB05 MOLM-13 C429(2.14); C309(3.49); C62(1.73); C405(3.18)  LDD3333  [6]
 LDCM0131  RA190 MM1.R C62(1.23)  LDD0304  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase 1 (MAPK1) CMGC Ser/Thr protein kinase family P28482
Germ cell-less protein-like 1 (GMCL1) . Q96IK5
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Coiled-coil domain-containing protein 57 (CCDC57) . Q2TAC2

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
4 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
7 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.