Details of the Target

General Information of Target

Target ID LDTP02700
Target Name Beta-enolase (ENO3)
Gene Name ENO3
Gene ID 2027
Synonyms
Beta-enolase; EC 4.2.1.11; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGK
GVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCK
AGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE
AMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKV
VIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDD
WATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLA
QSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK
AIFAGRKFRNPKAK
Target Bioclass
Enzyme
Family
Enolase family
Subcellular location
Cytoplasm
Function Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration.
Uniprot ID
P13929
Ensemble ID
ENST00000323997.10
HGNC ID
HGNC:3354

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K202(16.23)  LDD2217  [1]
DBIA
 Probe Info  		C357(0.75)  LDD3310  [2]
BTD
 Probe Info  		C339(0.78)  LDD2093  [3]
HHS-475
 Probe Info  		Y44(0.93)  LDD0264  [4]
HHS-465
 Probe Info  		Y131(8.62); Y257(10.00); Y44(7.91)  LDD2237  [5]
Acrolein
 Probe Info  		H25(0.00); C339(0.00)  LDD0221  [6]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [7]
WYneN
 Probe Info  		N.A.  LDD0021  [8]
ENE
 Probe Info  		C357(0.00); C399(0.00)  LDD0006  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0149  [9]
Ox-W18
 Probe Info  		N.A.  LDD2175  [10]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		1.00  LDD0139  [11]
STS-1
 Probe Info  		N.A.  LDD0069  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C339(1.20)  LDD2152  [3]
 LDCM0025  4SU-RNA HEK-293T C399(2.47)  LDD0371  [13]
 LDCM0020  ARS-1620 HCC44 C357(1.22)  LDD2171  [14]
 LDCM0116  HHS-0101 DM93 Y44(0.93)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y44(0.86)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y44(0.79)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y44(0.84)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y44(0.93)  LDD0268  [4]
 LDCM0107  IAA HeLa H25(0.00); C339(0.00)  LDD0221  [6]
 LDCM0022  KB02 22RV1 C357(1.33)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C357(1.70)  LDD2660  [2]
 LDCM0024  KB05 COLO792 C357(0.75)  LDD3310  [2]
 LDCM0109  NEM HeLa N.A.  LDD0224  [6]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C339(0.78)  LDD2093  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C339(2.24)  LDD2099  [3]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C339(2.23)  LDD2101  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C339(0.99)  LDD2109  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C339(0.92)  LDD2111  [3]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C339(0.60)  LDD2115  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C339(1.44)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C339(0.72)  LDD2127  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C339(0.77)  LDD2129  [3]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C339(1.27)  LDD2133  [3]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C339(0.62)  LDD2135  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C337(1.11)  LDD2140  [3]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C337(0.91)  LDD2143  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C339(1.37)  LDD2144  [3]
 LDCM0021  THZ1 HCT 116 C357(1.22)  LDD2173  [14]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2-phosphoglycolic Acid Small molecular drug DB02726
Phosphoenolpyruvate Small molecular drug DB01819
2-phospho-d-glyceric Acid . DB01709
Phosphonoacetohydroxamic Acid . DB03645

References

1 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
10 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
11 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
12 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.
13 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
14 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.