Details of the Target

General Information of Target

Target ID LDTP00754
Target Name Ras GTPase-activating protein 4 (RASA4)
Gene Name RASA4
Gene ID 10156
Synonyms
CAPRI; GAPL; KIAA0538; Ras GTPase-activating protein 4; Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVH
LPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEI
HLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRW
NETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSK
SRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTST
ECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSMESFLKVAG
MQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHL
GALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIM
SPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLK
DFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEA
LSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNE
LNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDP
LDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKVPEDSLARLLRVLQDLR
EAHSSSPAGSPPSEPNCLLELQT
Target Bioclass
Other
Subcellular location
Cytoplasm, cytosol
Function
Ca(2+)-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.
Uniprot ID
O43374
Ensemble ID
ENST00000262940.12
HGNC ID
HGNC:23181

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C705(0.97); C396(1.17)  LDD2183  [1]
DBIA
 Probe Info  		C434(0.87)  LDD1573  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C434(0.87)  LDD1573  [2]
 LDCM0373  CL104 HEK-293T C434(1.02)  LDD1577  [2]
 LDCM0377  CL108 HEK-293T C434(1.00)  LDD1581  [2]
 LDCM0382  CL112 HEK-293T C434(0.93)  LDD1586  [2]
 LDCM0386  CL116 HEK-293T C434(1.07)  LDD1590  [2]
 LDCM0391  CL120 HEK-293T C434(0.93)  LDD1595  [2]
 LDCM0395  CL124 HEK-293T C434(1.19)  LDD1599  [2]
 LDCM0399  CL128 HEK-293T C434(0.92)  LDD1603  [2]
 LDCM0403  CL16 HEK-293T C434(0.95)  LDD1607  [2]
 LDCM0416  CL28 HEK-293T C434(1.00)  LDD1620  [2]
 LDCM0429  CL4 HEK-293T C434(1.01)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C434(0.86)  LDD1634  [2]
 LDCM0443  CL52 HEK-293T C434(0.94)  LDD1646  [2]
 LDCM0456  CL64 HEK-293T C434(1.26)  LDD1659  [2]
 LDCM0469  CL76 HEK-293T C434(1.01)  LDD1672  [2]
 LDCM0482  CL88 HEK-293T C434(0.98)  LDD1685  [2]
 LDCM0625  F8 Ramos C705(1.54); C657(0.98); C797(0.88); C396(0.91)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C705(0.64); C797(1.42)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C797(0.52); C655(2.45)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C705(0.47); C657(4.35); C797(1.47)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C705(0.49); C797(0.69)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C657(0.87); C797(1.12); C396(0.66); C655(0.67)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C705(0.60); C797(2.73)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C705(0.58); C657(1.14); C797(0.72)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C705(0.61); C657(1.30); C797(0.72)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C705(0.40); C657(0.63); C797(0.60)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C705(0.39); C657(2.36); C797(1.10); C396(1.18)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C705(0.97); C657(2.47); C797(0.84)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C705(0.76); C797(0.69)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C705(0.53)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C705(0.41); C797(0.85)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C705(0.26); C657(1.89); C797(0.69)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C705(0.69); C797(1.30); C396(1.17); C655(0.93)  LDD2184  [1]
 LDCM0610  Fragment52 Ramos C705(1.10); C797(1.04)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C705(0.73); C657(1.62); C797(0.89)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C705(0.54); C797(1.88); C396(1.20); C655(1.98)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C705(0.77)  LDD2188  [1]
 LDCM0023  KB03 Ramos C705(0.97); C396(1.17)  LDD2183  [1]
 LDCM0024  KB05 Ramos C705(0.94); C655(0.83)  LDD2185  [1]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402