Details of the Target

General Information of Target

Target ID LDTP00447
Target Name Calpain-9 (CAPN9)
Gene Name CAPN9
Gene ID 10753
Synonyms
NCL4; Calpain-9; EC 3.4.22.-; Digestive tract-specific calpain; New calpain 4; nCL-4; Protein CG36
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSER
PQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQD
QSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKL
NGSYEALKGGSAIEAMEDFTGGVAETFQTKEAPENFYEILEKALKRGSLLGCFIDTRSAA
ESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQVEWNGSWSDSSPEWRSVG
PAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDALEEDAIHKWEVTVHQGSWVR
GSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGY
AIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEAD
FCLRIFSEKKAITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAE
ELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLF
LRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLVRLEN
ASRVFQALSTKNKEFIHLNINEFIHLTMNI
Target Bioclass
Enzyme
Family
Peptidase C2 family
Function Calcium-regulated non-lysosomal thiol-protease.
Uniprot ID
O14815
Ensemble ID
ENST00000271971.7
HGNC ID
HGNC:1486
ChEMBL ID
CHEMBL4523126

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.F665L .
CAL78 SNV: p.S238R .
DEL SNV: p.M535I .
HS839T SNV: p.M496V .
JURKAT SNV: p.V158I .
KYSE410 SNV: p.G182E .
KYSE510 SNV: p.G579R .
LS180 SNV: p.L416P .
MEWO SNV: p.G189E; p.G201E .
MFE319 SNV: p.C654Y .
SKMEL28 SNV: p.G366A .
SW1271 SNV: p.V144L .
WM793 SNV: p.L408V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C334(1.79)  LDD3360  [1]
BTD
 Probe Info  		C367(0.67)  LDD2115  [2]
AHL-Pu-1
 Probe Info  		C367(2.25)  LDD0168  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C367(2.25)  LDD0168  [3]
 LDCM0026  4SU-RNA+native RNA HEK-293T C367(20.00)  LDD0169  [3]
 LDCM0022  KB02 BRX142 C334(1.60)  LDD2267  [1]
 LDCM0023  KB03 A101D C334(3.20)  LDD2890  [1]
 LDCM0024  KB05 NCI-H3122 C334(1.79)  LDD3360  [1]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C367(0.67)  LDD2115  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C367(0.84)  LDD2133  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264