Details of the Target

General Information of Target

Target ID LDTP16265
Target Name FERM domain-containing protein 4B (FRMD4B)
Gene Name FRMD4B
Gene ID 23150
Synonyms
GRSP1; KIAA1013; FERM domain-containing protein 4B; GRP1-binding protein GRSP1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSPHPEAITDCVTLNTVGQLAEGGYPLRFSTLFQEQQKMNISQASVSFKDVTIEFTQEEW
QQMAPVQKNLYRDVMLENYSNLVSVGYCCFKPEVIFKLEQGEEPWFSEEEFSNQSHPKDY
RGDDLIKQNKKIKDKHLEQAICINNKTLTTEEEKVLGKPFTLHVAAVASTKMSCKCNSWE
VNLQSISEFIINNRNYSTKKIGCGNVCENSPFKINFEKTQTGEKFYEHNKNMKALNYNEN
LPKHPKFQTLEQAFECNKIGKAFNDKANCVKHNSSHTGETSSKDDEFRKNCDKKTLFDHR
RTGTGKKHLHLNQCGKSFEKSTVEEYNKLNMGIKHYELNPSGNNFNRKAHLTDPQTAVIE
ENPLVSNDRTQTWVKSSEYHENKKSYQTSVHRVRRRSHSMMKPYKCNECGKSFCQKGHLI
QHQRTHTGEKPFECSECGKTFSQKSHLSTHQRIHTAEKPYKCNECGKTFVQKSTLRGHQR
IHTGEKPYECSECGKTFVQKSTLRDHHRIHTGEKSFQCNQCGKTFGQKSNLRIHQRTHTG
EKTYQCNECEKSFWRKDHLIQHQKTHTGEKPFKCNECGKTFARTSTLRVHQRIHTGEKPF
KCNECGKKFVRKAILSDHQRIHTGEKPFQCNKCGKTFGQKSNLRIHQRTHSGEKSYECNE
YGKLCKKSTLSLYQKIQGEGNPY
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton
Function
Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex. Plays a redundant role with FRMD4A in epithelial polarization.
Uniprot ID
Q9Y2L6
Ensemble ID
ENST00000398540.8
HGNC ID
HGNC:24886

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.K615SfsTer129 .
AN3CA SNV: p.A517P .
DEL SNV: p.S699Y .
G415 SNV: p.K562E DBIA    Probe Info 
HCT116 SNV: p.A843S .
HCT15 SNV: p.K464N .
HGC27 Deletion: p.K795EfsTer21 .
KARPAS422 SNV: p.H40P .
KYSE150 SNV: p.D525H DBIA    Probe Info 
MELJUSO SNV: p.G16A DBIA    Probe Info 
MOLT4 SNV: p.N160K .
NCIH2291 SNV: p.G913E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C527(2.30)  LDD3319  [1]
AHL-Pu-1
 Probe Info  		C688(4.89)  LDD0171  [2]
IA-alkyne
 Probe Info  		C929, C939(20.00); C527(6.77)  LDD1704  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
IPM
 Probe Info  		C527(0.00); C688(0.00)  LDD2156  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C688(4.89)  LDD0171  [2]
 LDCM0022  KB02 T cell-activated C929, C939(20.00); C527(6.77)  LDD1704  [3]
 LDCM0023  KB03 A2058 C527(1.92)  LDD2670  [1]
 LDCM0024  KB05 MEWO C527(2.30)  LDD3319  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019