Details of the Target

General Information of Target

Target ID LDTP16135
Target Name Probable ATP-dependent RNA helicase DDX43 (DDX43)
Gene Name DDX43
Gene ID 55510
Synonyms
HAGE; Probable ATP-dependent RNA helicase DDX43; EC 3.6.4.13; Cancer/testis antigen 13; CT13; DEAD box protein 43; DEAD box protein HAGE; Helical antigen
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRGCLRLALLCALPWLLLAASPGHPAKSPRQPPAPRRDPFDAARGADFDHVYSGVVNLST
ENIYSFNYTSQPDQVTAVRVYVNSSSENLNYPVLVVVRQQKEVLSWQVPLLFQGLYQRSY
NYQEVSRTLCPSEATNETGPLQQLIFVDVASMAPLGAQYKLLVTKLKHFQLRTNVAFHFT
ASPSQPQYFLYKFPKDVDSVIIKVVSEMAYPCSVVSVQNIMCPVYDLDHNVEFNGVYQSM
TKKAAITLQKKDFPGEQFFVVFVIKPEDYACGGSFFIQEKENQTWNLQRKKNLEVTIVPS
IKESVYVKSSLFSVFIFLSFYLGCLLVGFVHYLRFQRKSIDGSFGSNDGSGNMVASHPIA
ASTPEGSNYGTIDESSSSPGRQMSSSDGGPPGQSDTDSSVEESDFDTMPDIESDKNIIRT
KMFLYLSDLSRKDRRIVSKKYKIYFWNIITIAVFYALPVIQLVITYQTVVNVTGNQDICY
YNFLCAHPLGVLSAFNNILSNLGHVLLGFLFLLIVLRRDILHRRALEAKDIFAVEYGIPK
HFGLFYAMGIALMMEGVLSACYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQTRHPDINAS
AYSAYASFAVVIMVTVLGVVFGKNDVWFWVIFSAIHVLASLALSTQIYYMGRFKIDLGIF
RRAAMVFYTDCIQQCSRPLYMDRMVLLVVGNLVNWSFALFGLIYRPRDFASYMLGIFICN
LLLYLAFYIIMKLRSSEKVLPVPLFCIVATAVMWAAALYFFFQNLSSWEGTPAESREKNR
ECILLDFFDDHDIWHFLSATALFFSFLVLLTLDDDLDVVRRDQIPVF
Target Bioclass
Enzyme
Family
DEAD box helicase family
Uniprot ID
Q9NXZ2
Ensemble ID
ENST00000370336.5
HGNC ID
HGNC:18677

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A375 SNV: p.I283M .
CCK81 SNV: p.R50H .
IGR1 SNV: p.T224A .
NCIH1155 SNV: p.M298I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C71(1.76); C143(1.77)  LDD3392  [1]
AHL-Pu-1
 Probe Info  		C249(2.38)  LDD0170  [2]
NAIA_5
 Probe Info  		N.A.  LDD2225  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C249(2.38)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA DM93 C143(2.35); C249(2.06)  LDD0171  [2]
 LDCM0632  CL-Sc Hep-G2 C71(20.00)  LDD2227  [3]
 LDCM0022  KB02 PANC-1 C71(1.24); C143(1.28)  LDD2558  [1]
 LDCM0023  KB03 PANC-1 C71(1.96); C143(1.40)  LDD2975  [1]
 LDCM0024  KB05 PANC-1 C71(1.76); C143(1.77)  LDD3392  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264