Details of the Target

General Information of Target

Target ID LDTP15189
Target Name WD repeat- and FYVE domain-containing protein 4 (WDFY4)
Gene Name WDFY4
Gene ID 57705
Synonyms
C10orf64; KIAA1607; WD repeat- and FYVE domain-containing protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLENYGAVASLAAFPFPKPALISQLERGETPWCSVPRGALDGEAPRGISSGYPFLKPAGI
SHPEQVEEPLNLKLQGEGPSLICPEGVLKRKKEDFILKEEIIEEAQDLMVLSSGPQWCGS
QELWFGKTCEEKSRLGRWPGYLNGGRMESSTNDIIEVIVKDEMISVEESSGNTDVNNLLG
IHHKILNEQIFYICEECGKCFDQNEDFDQHQKTHNGEKVYGCKECGKAFSFRSHCIAHQR
IHSGVKPYECQECAKAFVWKSNLIRHQRIHTGEKPFECKECGKGFSQNTSLTQHQRIHTG
EKPYTCKECGKSFTRNPALLRHQRMHTGEKPYECKDCGKGFMWNSDLSQHQRVHTGDKPH
ECTDCGKSFFCKAHLIRHQRIHTGERPYKCNDCGKAFSQNSVLIKHQRRHARDKPYNCQI
SHLLEH
Target Bioclass
Other
Subcellular location
Early endosome
Function
Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells. Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking. Also plays a role in B-cell survival through regulation of autophagy.
Uniprot ID
Q6ZS81
Ensemble ID
ENST00000325239.12
HGNC ID
HGNC:29323

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.Q450Ter .
A3KAW SNV: p.F2611C .
A498 SNV: p.P480T .
ABC1 SNV: p.K2451E .
AN3CA SNV: p.C890R; p.M1412T; p.A1666V .
BT549 SNV: p.R809L .
CHL1 SNV: p.L565V; p.G2825E .
COLO678 SNV: p.A104P .
COLO792 SNV: p.E1765K .
DU145 Deletion: p.A1979QfsTer36
Insertion: p.G1031RfsTer19
SNV: p.L343M;p.E1125K;p.V1641L		 .
ETK1 SNV: p.T896N .
HCC15 SNV: p.D1575Y; p.A1743V .
HCC827 SNV: p.Y2417H .
HCT116 SNV: p.Y138H; p.D1662N .
HCT15 SNV: p.L244I; p.L730M; p.P2083S; p.R2248M; p.V2941L .
HEC1B SNV: p.R94M .
HEPG2 SNV: p.P1494L .
HL60 SNV: p.T885P .
HT115 SNV: p.K1317N; p.A3063S .
IGR1 SNV: p.E3130Q .
IM95 SNV: p.S1933N .
JHH1 SNV: p.V1310E .
JHH6 Deletion: p.L1384Ter .
KPL1 SNV: p.V2122L .
MCC26 SNV: p.D2757N .
MCF7 SNV: p.V2122L .
MDAMB453 SNV: p.P966T .
MDAMB468 SNV: p.G1057V .
MELHO SNV: p.G2199E .
MEWO SNV: p.P1830L .
MFE296 Deletion: p.L1831WfsTer44
SNV: p.A1084S		 .
MFE319 SNV: p.A261T .
NCIH1703 SNV: p.E2308K .
NCIH1793 SNV: p.Q445H; p.Q981K .
NCIH2286 SNV: p.P842H .
OE33 SNV: p.N2586T .
PANC0403 SNV: p.P1729T .
RD SNV: p.K2393T .
RKO SNV: p.A1791T .
RL952 SNV: p.H945L .
SKMEL2 SNV: p.M1? .
SKN SNV: p.A599T .
SKNSH SNV: p.S2951Y .
SW1271 SNV: p.G2716W .
SW948 SNV: p.S805C .
TKKK SNV: p.E2683K .
U2OS SNV: p.K519N .
UMUC3 SNV: p.Q997P .
VMRCRCW SNV: p.S762R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C2510(4.28)  LDD3333  [1]
IA-alkyne
 Probe Info  		C1336(0.63); C1665(1.02)  LDD2182  [2]
Acrolein
 Probe Info  		N.A.  LDD0227  [3]
NAIA_4
 Probe Info  		C1182(0.00); C1177(0.00)  LDD2226  [4]
1d-yne
 Probe Info  		N.A.  LDD0356  [5]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C1336(1.14); C1665(0.85); C1696(0.79); C1963(1.11)  LDD2187  [2]
 LDCM0572  Fragment10 Ramos C1336(1.04); C1696(1.41); C1260(1.22)  LDD2189  [2]
 LDCM0573  Fragment11 Ramos C1336(0.16); C1696(0.50); C1260(2.46)  LDD2190  [2]
 LDCM0574  Fragment12 Ramos C1336(1.54); C1696(2.23); C1963(17.81)  LDD2191  [2]
 LDCM0575  Fragment13 Ramos C1336(1.04); C1696(1.15); C1963(1.47)  LDD2192  [2]
 LDCM0576  Fragment14 Ramos C1336(0.73); C1665(0.70); C1696(1.50)  LDD2193  [2]
 LDCM0579  Fragment20 Ramos C1336(1.28); C1696(2.33)  LDD2194  [2]
 LDCM0580  Fragment21 Ramos C1336(0.96); C1696(1.14); C1963(1.68); C1260(1.99)  LDD2195  [2]
 LDCM0582  Fragment23 Ramos C1336(0.79); C1696(0.65); C545(0.75); C1963(0.95)  LDD2196  [2]
 LDCM0578  Fragment27 Ramos C1336(1.34); C1696(0.78); C545(0.65); C1963(0.89)  LDD2197  [2]
 LDCM0586  Fragment28 Ramos C1336(0.62); C1665(1.54); C1696(1.40); C545(0.84)  LDD2198  [2]
 LDCM0588  Fragment30 Ramos C1336(1.66); C1696(1.24); C1963(1.66); C1260(2.18)  LDD2199  [2]
 LDCM0589  Fragment31 Ramos C1336(0.95); C1696(0.84); C545(1.81); C1963(0.91)  LDD2200  [2]
 LDCM0590  Fragment32 Ramos C1336(0.92); C1696(2.05); C1260(1.29)  LDD2201  [2]
 LDCM0468  Fragment33 Ramos C1336(1.06); C1696(1.23); C545(2.11); C1963(1.52)  LDD2202  [2]
 LDCM0596  Fragment38 Ramos C1336(1.22); C1696(1.08); C1260(0.73)  LDD2203  [2]
 LDCM0566  Fragment4 Ramos C1336(0.89); C1665(1.55)  LDD2184  [2]
 LDCM0610  Fragment52 Ramos C1336(1.67); C1696(1.98); C1260(0.86)  LDD2204  [2]
 LDCM0614  Fragment56 Ramos C1336(2.98); C1696(1.09); C1963(0.96); C1260(1.30)  LDD2205  [2]
 LDCM0569  Fragment7 Ramos C1336(0.84); C1665(1.82); C1696(1.40)  LDD2186  [2]
 LDCM0571  Fragment9 Ramos C1336(0.92)  LDD2188  [2]
 LDCM0022  KB02 Ramos C1336(0.63); C1665(1.02)  LDD2182  [2]
 LDCM0023  KB03 Ramos C1336(0.72); C1665(1.24); C1963(1.66)  LDD2183  [2]
 LDCM0024  KB05 MOLM-13 C2510(4.28)  LDD3333  [1]
 LDCM0109  NEM HeLa N.A.  LDD0227  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Optineurin (OPTN) . Q96CV9

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.