Details of the Target

General Information of Target

Target ID LDTP14832
Target Name 28 kDa heat- and acid-stable phosphoprotein (PDAP1)
Gene Name PDAP1
Gene ID 11333
Synonyms
HASPP28; 28 kDa heat- and acid-stable phosphoprotein; PDGF-associated protein; PAP; PDGFA-associated protein 1; PAP1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MAEAAAPGTTVTTSGAGAAAAEAAETAEAVSPTPIPTVTAPSPRAGGGVGGSDGSDGSGG
RGDSGAYDGSGACGGSDACDGSGDSSGDSWTKQVTCRYFKYGICKEGDNCRYSHDLSDRL
CGVVCKYFQRGCCVYGDRCRCEHSKPLKQEEATATELTTKSSLAASSSLSSIVGPLVEMN
TNEAESRNSNFATVVAGSEDWANAIEFVPGQPYCGRTVPSCTEAPLQGSVTKEESEEEQT
AVETKKQLCPYAAVGQCRYGENCVYLHGDLCDMCGLQVLHPMDAAQRSQHIQACIEAHEK
DMEFSFAVQRSKDKVCGICMEVVYEKANPNEHRFGILSNCNHTFCLKCIRKWRSAKEFES
RIVKSCPQCRITSNFVIPSEYWVEEKEEKQKLIQKYKEAMSNKACKYFDEGRGSCPFGEN
CFYKHMYPDGRREEPQRQQVGTSSRNPGQQRNHFWEFFEEGANSNPFDDEEEAVTFELGE
MLLML
Target Bioclass
Other
Family
PDAP1 family
Function Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB.
Uniprot ID
Q13442
Ensemble ID
ENST00000350498.8
HGNC ID
HGNC:14634

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		5.27  LDD0066  [1]
STPyne
 Probe Info  		K11(10.00); K126(1.59); K132(7.62); K137(10.00)  LDD0277  [2]
AZ-9
 Probe Info  		D82(1.11)  LDD2208  [3]
ONAyne
 Probe Info  		N.A.  LDD0273  [2]
OPA-S-S-alkyne
 Probe Info  		K75(0.22); K181(1.83)  LDD3494  [4]
Probe 1
 Probe Info  		Y17(55.37); Y70(186.88)  LDD3495  [5]
m-APA
 Probe Info  		10.68  LDD0403  [6]
ATP probe
 Probe Info  		K105(0.00); K95(0.00); K137(0.00); K132(0.00)  LDD0199  [7]
1d-yne
 Probe Info  		K52(0.00); K33(0.00)  LDD0358  [8]
ATP probe
 Probe Info  		K75(0.00); K132(0.00); K95(0.00); K11(0.00)  LDD0035  [9]
NHS
 Probe Info  		N.A.  LDD0010  [10]
YN-1
 Probe Info  		N.A.  LDD0447  [11]
Acrolein
 Probe Info  		N.A.  LDD0217  [12]
HHS-465
 Probe Info  		N.A.  LDD2240  [13]
HHS-475
 Probe Info  		Y17(0.57)  LDD2238  [14]
HHS-482
 Probe Info  		Y17(1.52)  LDD2239  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 10.68  LDD0403  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [12]
 LDCM0109  NEM HeLa N.A.  LDD0225  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase pellino homolog 1 (PELI1) Pellino family Q96FA3

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
7 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
8 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
9 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.