Details of the Target

General Information of Target

Target ID LDTP14299
Target Name Dual specificity protein phosphatase 10 (DUSP10)
Gene Name DUSP10
Gene ID 11221
Synonyms
MKP5; Dual specificity protein phosphatase 10; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 5; MAP kinase phosphatase 5; MKP-5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANT
FASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPV
LETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRK
EKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVEN
VDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHP
PPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPD
FAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPL
SDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVE
YSDSEDDSSEFDEDDWSD
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Subcellular location
Cytoplasm
Function Protein phosphatase involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily. It preferably dephosphorylates p38.
TTD ID
T17339
Uniprot ID
Q9Y6W6
DrugMap ID
TTF3RJ0
Ensemble ID
ENST00000366899.4
HGNC ID
HGNC:3065
ChEMBL ID
CHEMBL2396511

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.I46V .
HEC1 SNV: p.A99T .
HEC1B SNV: p.A99T .
MCC13 SNV: p.E265K .
MCC26 Substitution: p.H407Y .
MEWO SNV: p.P3L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C218(2.76)  LDD3331  [1]
AHL-Pu-1
 Probe Info  		C293(2.53)  LDD0170  [2]
IA-alkyne
 Probe Info  		C218(6.03)  LDD1703  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C293(2.53)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA DM93 C293(2.07)  LDD0171  [2]
 LDCM0022  KB02 T cell C218(6.03)  LDD1703  [3]
 LDCM0023  KB03 A431 C218(2.43)  LDD2675  [1]
 LDCM0024  KB05 MKN-1 C218(2.76)  LDD3331  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Beta-secretase 2 (BACE2) Peptidase A1 family Q9Y5Z0
Mitogen-activated protein kinase 9 (MAPK9) CMGC Ser/Thr protein kinase family P45984
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
GTPase HRas (HRAS) Ras family P01112
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamate receptor ionotropic, NMDA 2C (GRIN2C) Glutamate-gated ion channel family Q14957
Alpha-crystallin A chain (CRYAA) Small heat shock protein (HSP20) family P02489
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) Dynein heavy chain family Q14204
Keratin-associated protein 3-2 (KRTAP3-2) KRTAP type 3 family Q9BYR7
Gelsolin (GSN) Villin/gelsolin family P06396
Ubiquilin-1 (UBQLN1) . Q9UMX0

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.