Details of the Target

General Information of Target

Target ID LDTP14130
Target Name Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5)
Gene Name UCHL5
Gene ID 51377
Synonyms
UCH37; Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH-L5; EC 3.4.19.12; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPVVTTDAESETGIPKSLSNEPPSETMEEIEHTCPQPRLTLTAPAPFADETNCQCQAPHE
KLTIAQARLGTPADRPVRVYADGIFDLFHSGHARALMQAKTLFPNSYLLVGVCSDDLTHK
FKGFTVMNEAERYEALRHCRYVDEVIRDAPWTLTPEFLEKHKIDFVAHDDIPYSSAGSDD
VYKHIKEAGMFVPTQRTEGISTSDIITRIVRDYDVYARRNLQRGYTAKELNVSFINEKRY
RFQNQVDKMKEKVKNVEERSKEFVNRVEEKSHDLIQKWEEKSREFIGNFLELFGPDGAWK
QMFQERSSRMLQALSPKQSPVSSPTRSRSPSRSPSPTFSWLPLKTSPPSSPKAASASISS
MSEGDEDEK
Target Type
Patented-recorded
Target Bioclass
Enzyme
Family
Peptidase C12 family
Subcellular location
Cytoplasm
Function
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
TTD ID
T15441
Uniprot ID
Q9Y5K5
DrugMap ID
TTSX29Z
Ensemble ID
ENST00000367448.5
HGNC ID
HGNC:19678
ChEMBL ID
CHEMBL3407316

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y176(20.00); Y211(5.20)  LDD0257  [2]
STPyne
 Probe Info  		K282(8.16)  LDD0277  [3]
Probe 1
 Probe Info  		Y176(33.70); Y211(23.05); Y231(28.36)  LDD3495  [4]
DBIA
 Probe Info  		C191(1.94)  LDD3319  [5]
5E-2FA
 Probe Info  		H164(0.00); H306(0.00)  LDD2235  [6]
1d-yne
 Probe Info  		N.A.  LDD0358  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C191(0.00); C100(0.00); C88(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [9]
IPIAA_H
 Probe Info  		C88(0.00); C191(0.00)  LDD0030  [10]
IPIAA_L
 Probe Info  		C88(0.00); C191(0.00)  LDD0031  [10]
Lodoacetamide azide
 Probe Info  		C191(0.00); C100(0.00); C88(0.00)  LDD0037  [8]
Compound 10
 Probe Info  		N.A.  LDD2216  [11]
IPM
 Probe Info  		C191(0.00); C88(0.00)  LDD2156  [12]
Acrolein
 Probe Info  		C191(0.00); C9(0.00)  LDD0217  [13]
W1
 Probe Info  		N.A.  LDD0236  [14]
AOyne
 Probe Info  		15.00  LDD0443  [15]
NAIA_5
 Probe Info  		C191(0.00); C9(0.00)  LDD2223  [16]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe13
 Probe Info  		20.00  LDD0475  [17]
FFF probe2
 Probe Info  		9.04  LDD0463  [17]
FFF probe3
 Probe Info  		20.00  LDD0464  [17]
STS-2
 Probe Info  		N.A.  LDD0139  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 12.19  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa C191(0.00); C9(0.00)  LDD0222  [13]
 LDCM0632  CL-Sc Hep-G2 C191(0.69)  LDD2227  [16]
 LDCM0625  F8 Ramos C191(0.59)  LDD2187  [19]
 LDCM0572  Fragment10 Ramos C191(0.63)  LDD2189  [19]
 LDCM0573  Fragment11 Ramos C191(1.91)  LDD2190  [19]
 LDCM0574  Fragment12 Ramos C191(0.57)  LDD2191  [19]
 LDCM0575  Fragment13 Ramos C191(1.40)  LDD2192  [19]
 LDCM0576  Fragment14 Ramos C191(0.63)  LDD2193  [19]
 LDCM0579  Fragment20 Ramos C191(0.59)  LDD2194  [19]
 LDCM0580  Fragment21 Ramos C191(0.73)  LDD2195  [19]
 LDCM0582  Fragment23 Ramos C191(0.55)  LDD2196  [19]
 LDCM0578  Fragment27 Ramos C191(0.77)  LDD2197  [19]
 LDCM0586  Fragment28 Ramos C191(0.79)  LDD2198  [19]
 LDCM0588  Fragment30 Ramos C191(1.01)  LDD2199  [19]
 LDCM0589  Fragment31 Ramos C191(0.93)  LDD2200  [19]
 LDCM0590  Fragment32 Ramos C191(0.51)  LDD2201  [19]
 LDCM0468  Fragment33 Ramos C191(1.10)  LDD2202  [19]
 LDCM0596  Fragment38 Ramos C191(0.65)  LDD2203  [19]
 LDCM0566  Fragment4 Ramos C191(0.71)  LDD2184  [19]
 LDCM0610  Fragment52 Ramos C191(1.17)  LDD2204  [19]
 LDCM0614  Fragment56 Ramos C191(1.23)  LDD2205  [19]
 LDCM0569  Fragment7 Ramos C191(0.49)  LDD2186  [19]
 LDCM0571  Fragment9 Ramos C191(0.47)  LDD2188  [19]
 LDCM0107  IAA HeLa C191(0.00); H306(0.00)  LDD0221  [13]
 LDCM0022  KB02 Ramos C191(0.52)  LDD2182  [19]
 LDCM0023  KB03 Ramos C191(0.62)  LDD2183  [19]
 LDCM0024  KB05 MEWO C191(1.94)  LDD3319  [5]
 LDCM0109  NEM HeLa N.A.  LDD0223  [13]
 LDCM0131  RA190 MM1.R C191(1.38)  LDD0304  [20]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome subunit alpha type-6 (PSMA6) Peptidase T1A family P60900
26S proteasome non-ATPase regulatory subunit 2 (PSMD2) Proteasome subunit S2 family Q13200
26S proteasome non-ATPase regulatory subunit 4 (PSMD4) Proteasome subunit S5A family P55036
E3 SUMO-protein ligase ZBED1 (ZBED1) . O96006
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Actin-related protein 8 (ACTR8) Actin family Q9H981
Proteasomal ubiquitin receptor ADRM1 (ADRM1) ADRM1 family Q16186
Nuclear factor related to kappa-B-binding protein (NFRKB) NFRKB family Q6P4R8
INO80 complex subunit E (INO80E) . Q8NBZ0
TCF3 fusion partner (TFPT) . P0C1Z6

The Drug(s) Related To This Target

Patented
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Pmid26077642-compound-figure3a Small molecular drug D0A8EN

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
11 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
12 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
15 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
18 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
19 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
20 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.