Details of the Target

General Information of Target

Target ID LDTP14123
Target Name Hairy/enhancer-of-split related with YRPW motif protein 1 (HEY1)
Gene Name HEY1
Gene ID 23462
Synonyms
BHLHB31; CHF2; HERP2; HESR1; HRT1; Hairy/enhancer-of-split related with YRPW motif protein 1; Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; bHLHb31; HES-related repressor protein 1; Hairy and enhancer of split-related protein 1; HESR-1; Hairy-related transcription factor 1; HRT-1; hHRT1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MPPERRRRMKLDRRTGAKPKRKPGMRPDWKAGAGPGGPPQKPAPSSQRKPPARPSAAAAA
IAVAAAEEERRLRQRNRLRLEEDKPAVERCLEELVFGDVENDEDALLRRLRGPRVQEHED
SGDSEVENEAKGNFPPQKKPVWVDEEDEDEEMVDMMNNRFRKDMMKNASESKLSKDNLKK
RLKEEFQHAMGGVPAWAETTKRKTSSDDESEEDEDDLLQRTGNFISTSTSLPRGILKMKN
CQHANAERPTVARISSVQFHPGAQIVMVAGLDNAVSLFQVDGKTNPKIQSIYLERFPIFK
ACFSANGEEVLATSTHSKVLYVYDMLAGKLIPVHQVRGLKEKIVRSFEVSPDGSFLLING
IAGYLHLLAMKTKELIGSMKINGRVAASTFSSDSKKVYASSGDGEVYVWDVNSRKCLNRF
VDEGSLYGLSIATSRNGQYVACGSNCGVVNIYNQDSCLQETNPKPIKAIMNLVTGVTSLT
FNPTTEILAIASEKMKEAVRLVHLPSCTVFSNFPVIKNKNISHVHTMDFSPRSGYFALGN
EKGKALMYRLHHYSDF
Target Bioclass
Transporter and channel
Family
HEY family
Subcellular location
Nucleus
Function
Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. Involved in the regulation of liver cancer cells self-renewal.
Uniprot ID
Q9Y5J3
Ensemble ID
ENST00000337919.9
HGNC ID
HGNC:4880

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KYSE510 SNV: p.R218S .
MCC26 SNV: p.F264L .
OSRC2 SNV: p.T245I .
SUPT1 SNV: p.E24G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C130(0.87)  LDD1492  [1]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C130(1.22)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C130(0.92)  LDD1510  [1]
 LDCM0278  AC19 HEK-293T C130(0.92)  LDD1517  [1]
 LDCM0280  AC20 HEK-293T C130(0.63)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C130(1.05)  LDD1520  [1]
 LDCM0287  AC27 HEK-293T C130(0.84)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C130(0.79)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C130(1.09)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C130(1.04)  LDD1529  [1]
 LDCM0296  AC35 HEK-293T C130(1.14)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C130(0.89)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C130(1.13)  LDD1537  [1]
 LDCM0301  AC4 HEK-293T C130(0.93)  LDD1540  [1]
 LDCM0305  AC43 HEK-293T C130(1.27)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C130(1.28)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C130(1.08)  LDD1546  [1]
 LDCM0312  AC5 HEK-293T C130(1.10)  LDD1551  [1]
 LDCM0314  AC51 HEK-293T C130(1.14)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C130(0.86)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C130(1.10)  LDD1555  [1]
 LDCM0322  AC59 HEK-293T C130(0.92)  LDD1561  [1]
 LDCM0324  AC60 HEK-293T C130(0.79)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C130(1.15)  LDD1564  [1]
 LDCM0248  AKOS034007472 HEK-293T C130(1.10)  LDD1511  [1]
 LDCM0367  CL1 HEK-293T C130(0.92)  LDD1571  [1]
 LDCM0370  CL101 HEK-293T C130(0.95)  LDD1574  [1]
 LDCM0374  CL105 HEK-293T C130(0.92)  LDD1578  [1]
 LDCM0378  CL109 HEK-293T C130(1.04)  LDD1582  [1]
 LDCM0383  CL113 HEK-293T C130(1.08)  LDD1587  [1]
 LDCM0387  CL117 HEK-293T C130(1.16)  LDD1591  [1]
 LDCM0392  CL121 HEK-293T C130(1.02)  LDD1596  [1]
 LDCM0396  CL125 HEK-293T C130(1.01)  LDD1600  [1]
 LDCM0400  CL13 HEK-293T C130(0.95)  LDD1604  [1]
 LDCM0406  CL19 HEK-293T C130(1.24)  LDD1610  [1]
 LDCM0408  CL20 HEK-293T C130(0.91)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C130(1.25)  LDD1613  [1]
 LDCM0413  CL25 HEK-293T C130(1.11)  LDD1617  [1]
 LDCM0420  CL31 HEK-293T C130(0.98)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C130(0.92)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C130(1.08)  LDD1626  [1]
 LDCM0426  CL37 HEK-293T C130(0.90)  LDD1630  [1]
 LDCM0433  CL43 HEK-293T C130(1.14)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C130(1.09)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C130(1.06)  LDD1639  [1]
 LDCM0439  CL49 HEK-293T C130(1.14)  LDD1643  [1]
 LDCM0446  CL55 HEK-293T C130(1.41)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C130(1.09)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C130(1.13)  LDD1651  [1]
 LDCM0453  CL61 HEK-293T C130(1.07)  LDD1656  [1]
 LDCM0459  CL67 HEK-293T C130(1.23)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C130(0.91)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C130(1.38)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C130(1.18)  LDD1665  [1]
 LDCM0466  CL73 HEK-293T C130(1.07)  LDD1669  [1]
 LDCM0472  CL79 HEK-293T C130(0.93)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C130(2.15)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C130(0.86)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C130(1.12)  LDD1678  [1]
 LDCM0479  CL85 HEK-293T C130(0.90)  LDD1682  [1]
 LDCM0484  CL9 HEK-293T C130(1.10)  LDD1687  [1]
 LDCM0486  CL91 HEK-293T C130(1.01)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C130(0.92)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C130(1.24)  LDD1691  [1]
 LDCM0492  CL97 HEK-293T C130(0.90)  LDD1695  [1]
 LDCM0022  KB02 HEK-293T C130(0.87)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C130(0.98)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C130(0.85)  LDD1502  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Terminal nucleotidyltransferase 5D (TENT5D) TENT family Q8NEK8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Forkhead box protein H1 (FOXH1) . O75593
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Keratin-associated protein 6-2 (KRTAP6-2) KRTAP type 6 family Q3LI66
YTH domain-containing family protein 1 (YTHDF1) YTHDF family Q9BYJ9
DAZ-associated protein 2 (DAZAP2) . Q15038
Pleckstrin homology domain-containing family B member 2 (PLEKHB2) . Q96CS7

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004