Details of the Target

General Information of Target

Target ID LDTP13735
Target Name Actin-binding protein WASF3 (WASF3)
Gene Name WASF3
Gene ID 10810
Synonyms
KIAA0900; SCAR3; WAVE3; Actin-binding protein WASF3; Protein WAVE-3; Verprolin homology domain-containing protein 3; Wiskott-Aldrich syndrome protein family member 3; WASP family protein member 3
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MVRKPVVSTISKGGYLQGNVNGRLPSLGNKEPPGQEKVQLKRKVTLLRGVSIIIGTIIGA
GIFISPKGVLQNTGSVGMSLTIWTVCGVLSLFGALSYAELGTTIKKSGGHYTYILEVFGP
LPAFVRVWVELLIIRPAATAVISLAFGRYILEPFFIQCEIPELAIKLITAVGITVVMVLN
SMSVSWSARIQIFLTFCKLTAILIIIVPGVMQLIKGQTQNFKDAFSGRDSSITRLPLAFY
YGMYAYAGWFYLNFVTEEVENPEKTIPLAICISMAIVTIGYVLTNVAYFTTINAEELLLS
NAVAVTFSERLLGNFSLAVPIFVALSCFGSMNGGVFAVSRLFYVASREGHLPEILSMIHV
RKHTPLPAVIVLHPLTMIMLFSGDLDSLLNFLSFARWLFIGLAVAGLIYLRYKCPDMHRP
FKVPLFIPALFSFTCLFMVALSLYSDPFSTGIGFVITLTGVPAYYLFIIWDKKPRWFRIM
SEKITRTLQIILEVVPEEDKL
Target Bioclass
Other
Family
SCAR/WAVE family
Subcellular location
Cytoplasm, cytoskeleton
Function
Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
Uniprot ID
Q9UPY6
Ensemble ID
ENST00000335327.6
HGNC ID
HGNC:12734
ChEMBL ID
CHEMBL5169205

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Deletion: p.K456RfsTer61 .
HCT116 SNV: p.Q311H .
HEC1 SNV: p.R202C; p.V360A .
HEC1B SNV: p.R202C; p.V360A .
KMCH1 SNV: p.P10A .
KMRC1 SNV: p.D496N .
MOLT4 SNV: p.S257P .
NCIH1048 SNV: p.Y337C .
OVTOKO SNV: p.R480W .
P31FUJ SNV: p.P349S .
RKO SNV: p.K102E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C28(0.79)  LDD3321  [1]
NAIA_5
 Probe Info  		N.A.  LDD2223  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C28(1.01)  LDD1510  [3]
 LDCM0280  AC20 HEK-293T C28(0.90)  LDD1519  [3]
 LDCM0288  AC28 HEK-293T C28(0.93)  LDD1527  [3]
 LDCM0297  AC36 HEK-293T C28(0.93)  LDD1536  [3]
 LDCM0301  AC4 HEK-293T C28(1.09)  LDD1540  [3]
 LDCM0306  AC44 HEK-293T C28(0.93)  LDD1545  [3]
 LDCM0315  AC52 HEK-293T C28(0.98)  LDD1554  [3]
 LDCM0324  AC60 HEK-293T C28(0.96)  LDD1563  [3]
 LDCM0408  CL20 HEK-293T C28(1.13)  LDD1612  [3]
 LDCM0421  CL32 HEK-293T C28(1.06)  LDD1625  [3]
 LDCM0434  CL44 HEK-293T C28(0.96)  LDD1638  [3]
 LDCM0447  CL56 HEK-293T C28(1.14)  LDD1650  [3]
 LDCM0460  CL68 HEK-293T C28(1.02)  LDD1663  [3]
 LDCM0473  CL8 HEK-293T C28(1.04)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C28(1.06)  LDD1677  [3]
 LDCM0487  CL92 HEK-293T C28(0.98)  LDD1690  [3]
 LDCM0022  KB02 22RV1 C28(1.08)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C28(1.10)  LDD2660  [1]
 LDCM0024  KB05 SH4 C28(0.79)  LDD3321  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402